Raptor SPP Core-Shell LC Columns

A new species of column.

Welcome
  • Higher efficiency for drastically faster analysis times.
  • Better selectivity for substantially improved resolution.
  • Increased sample throughput with existing HPLC instrumentation.
  • Long-lasting ruggedness for dependable reproducibility.
Overview

The Dawn of an Era

Superficially porous particles (commonly referred to as SPP or “core-shell” particles) have been proven to provide fast separations without the need for expensive Ultra High Performance Liquid Chromatography (UHPLC) instruments, thereby increasing sample throughput without capital investment. These particles feature a solid, impermeable core enveloped by a thin, porous layer of silica that decreases the diffusion path and reduces peak dispersion. As a result, they offer significantly higher efficiency than traditional fully porous particles of similar dimensions—often rivaling the efficiency of smaller particles. Core-shell particles changed LC, but they were only the beginning....

A New Species Has Evolved

Restek is proud to announce that SPP core-shell technology has evolved with the introduction of Raptor LC columns. Although column efficiency, which is boosted with superficially porous particles, considerably accelerates analysis time, it has little effect on resolution (i.e., peak separation). Selectivity, on the other hand, has a substantial impact on resolution, but shows minimal improvement in analysis times. New Raptor LC columns bond rugged 2.7 and 5 μm superficially porous particles with Restek’s unique Ultra Selective Liquid Chromatography (USLC) phases to offer chromatographers the best of both worlds.

By being the first to combine the speed of SPP with the resolution of highly selective USLC technology, Raptor LC columns provide the practicing analyst with the most powerful tools available for fast and efficient method development. And because they are from Restek, Raptor LC columns are backed by the manufacturing and quality systems you’ve come to trust along with the best Plus 1 service in the industry. Choose them for all of your valued assays to experience Selectivity Accelerated.

Restek Literature Signup

Only Raptor LC columns
offer the higher efficiency of
a superficially porous particle
plus the improved resolution of
the USLC phases.

Fully porous particles with typical stationary
phases show longer retention times and less resolution.

Ordinary superficially porous particles (SPP) with
typical stationary phases show shorter retention
times than fully porous particles, but still exhibit less resolution.

Only Raptor LC columns with highly selective
USLC stationary phases show shorter
retention times and greater resolution.

Restek USLC Technology
Products

Raptor EtG/EtS LC Column

  • Proven performance for accurate, reliable ethyl glucuronide (EtG) and ethyl sulfate (EtS) analysis.
  • Strong retention consistently resolves analytes from matrix interferences.
  • Long column lifetime ensures consistent performance injection after injection.
  • Fast, 4-minute, dilute-and-shoot LC-MS/MS analysis supports high sample throughput.

Raptor HILIC-Si LC Columns (USP L3)

  • HILIC silica column designed for increased retention of polar compounds without ion-pairing reagents.
  • 2.7 µm Raptor core-shell particles provide the speed of SPP, with greater efficiency and capacity than 5 µm particles.
  • Ideal for increasing sensitivity and selectivity in LC-MS analyses.
  • Fully compatible with both HPLC and UHPLC when using low backpressure HILIC mobile phases.
  • Switch to a Raptor HILIC-Si LC column when you need more retention and resolution of hydrophilic, polar analytes.

Raptor FluoroPhenyl LC Columns (USP L43)

  • Capable of both reversed-phase and HILIC separations.
  • Ideal for increasing sensitivity and selectivity in LC-MS analyses.
  • Offers increased retention for charged bases.
  • Part of Restek’s Raptor LC column line featuring 2.7 and 5 μm SPP core-shell silica.
  • Switch to a Raptor FluoroPhenyl LC column when you need more retention and selectivity for basic and hydrophilic compounds than you can achieve on a C18.

Raptor C18 LC Columns (USP L1)

  • A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography.
  • Wide pH range (2–8) provides excellent data quality for many applications, matrices, and compounds.
  • Offers the highest hydrophobic retention of any Raptor phase.
  • Part of Restek’s Raptor LC column line featuring 2.7 and 5 μm SPP core-shell silica.

Raptor ARC-18 LC Columns (USP L1)

  • Ideal for high-throughput LC-MS/MS applications with minimal sample preparation.
  • Well-balanced retention profile for better detection and integration of large, multiclass analyte lists.
  • Sterically protected to endure low-pH mobile phases without sacrificing retention or peak quality.
  • Part of Restek’s Raptor LC column line featuring 2.7 and 5 µm SPP core-shell silica.

Raptor Biphenyl LC Columns (USP L11)

  • Ideal for bioanalytical testing applications like drug and metabolite analyses.
  • Heightened selectivity and retention for compounds that are hard to resolve or elute early on C18 and other phenyl chemistries.
  • Limits ionization suppression and allows simple, MS-friendly mobile phases.
  • Restek’s most popular LC stationary phase applied to 2.7 and 5 µm Raptor SPP core-shell silica.

Raptor EXP Guard Column Cartridges

  • Free-Turn architecture lets you change cartridges by hand without breaking inlet/outlet fluid connectionsno tools needed.
  • Patented titanium hybrid ferrules can be installed repeatedly without compromising high-pressure seal.
  • Auto-adjusting design provides ZDV (zero dead volume) connection to any 10-32 female port.

EXP Direct Connect Holder

  • Free-Turn architecture lets you change cartridges by hand without breaking inlet/outlet fluid connectionsno tools needed.
  • Patented titanium hybrid ferrules can be installed repeatedly without compromising high-pressure seal.
  • Auto-adjusting design provides ZDV (zero dead volume) connection to any 10-32 female port.
  • EXP direct connect holder requires separate guard column cartridges; available from Restek in 2.1, 3.0, and 4.6 mm.

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Hand-Tight Fittings

  • Hand-tight fitting style achieves effortless HPLC sealsno tools needed for a 8,700+ psi seal.
  • Both hand-tight and hex-head styles wrench-tighten for reliable UHPLC use up to 20,000+ psi!
  • Patented ferrule can be installed repeatedly without compromising high-pressure seal.
  • Hybrid design combines the durability of titanium with the sealing ability of PEEK.
  • Cutting-edge system provides ZDV (zero dead volume) connection to any 10-32 female port.
  • Compatible with 1/16" PEEK and stainless steel tubing.
Resources

Literature

Featured Application: Analysis of Melamine and Related Compounds on Raptor HILIC-Si - Trace-Level Analysis of Melamine and Related Compounds by LC-MS/MS

The analysis of melamine and related compounds is critical in protecting consumers from adulterated food products. While these highly polar analytes are difficult to retain and accurately quantify on many columns, the Raptor HILIC-Si column and LC-MS/MS method shown here allow quick and complete separations, so melamine and related compounds can be accurately identified and quantified even at residual levels.

Featured Application: Paraquat and Diquat on Raptor HILIC-Si - LC-MS/MS Analysis of Paraquat and Diquat without Ion-Pairing Reagents

LC-MS/MS analysis of paraquat and diquat herbicides is an important tool for protecting human and environmental health. However, the ion-pairing reagents that are commonly used to improve retention, resolution, and peak shape have a negative impact on sensitivity and can contaminate the system necessitating frequent cleaning. The LC-MS/MS analysis of paraquat and diquat presented here provides complete separation and good peak response for both target analytes in a fast, 7-minute gradient run without the use of troublesome ion pairing reagents.

Featured Application: Mycotoxins on Raptor FluoroPhenyl - Highly Selective LC-MS/MS Analysis of Mycotoxins from Multiple Classes

Mycotoxin contamination of crops is not only a public health concern, but it also has a huge impact on the global economy. This highly selective LC-MS/MS analysis of 20 mycotoxins in just 9 minutes allows scientists to accurately identify toxins, to monitor food products for contamination, and to support control strategies that prevent economic loss.

Featured Application: Folate Deficiency Biomarkers in Plasma on Raptor HILIC-Si - Fast, Accurate LC-MS/MS Method for Folate Deficiency Biomarkers in Plasma

LC-MS/MS methods for folate deficiency biomarkers in plasma are complicated by the presence of matrix phospholipids and lysophospholipids. These interferences are problematic with reversed-phase LC because they can suppress response, reduce accuracy, and contaminate the MS source. Using a Raptor HILIC-Si column and the HILIC method shown here, folate deficiency biomarkers are completely separated from matrix interferences, allowing accurate determinations to be made in a fast, 5-minute analysis.

Tech Tip: Column Conditioning Ensures Consistent EtG/EtS Results

Methods for monitoring alcohol consumption biomarkers EtG and EtS are generally limited by poor retention and coelution with matrix interferences, as well as by long analysis times and short column lifetimes. The dilute-and-shoot EtG/EtS LC-MS/MS analysis developed here using the novel Raptor EtG/EtS column easily resolves EtG and EtS from matrix interferences, providing consistent, accurate results for high-throughput labs testing human urine samples for alcohol consumption.

Definitive EtG/EtS LC-MS/MS Analysis: A Rugged 4-Min Method for High-Throughput Labs

By properly conditioning new columns and employing Restek’s EtG/EtS method for sample prep and analysis, high-throughput labs testing human urine samples for these alcohol consumption biomarkers can ensure accurate, consistent results with fewer column changes.

How to Avoid Common Problems with HILIC Methods

Whether you’ve run HILIC methods before or are attempting to for the first time, there are significant differences between reversed-phase and HILIC methods that must be considered. This article addresses common problems that analysts encounter when running HILIC methods and details how to avoid them and successfully use the technique.

“The Big Pain”: Development of Pain-Free Methods for Analyzing 231 Multiclass Drugs and Metabolites by LC-MS/MS

As the demand for testing of pain management drugs increases, many laboratories are turning to LC-MS/MS for its increased speed, sensitivity, and specificity. The methods shown here use a Raptor™ Biphenyl column because it provides fast, accurate analysis of 231 drugs and drug metabolites.

The Effects of LC Particle Choice on Column Performance: Switching from 3 and 5 µm Fully Porous Particles (FPP) to 5 µm Superficially Porous Particles (SPP)

In this technical note, we will compare the performance of Raptor 5 µm superficially porous particle (SPP) LC columns to traditional 3 and 5 µm fully porous particle (FPP) LC columns. We will demonstrate how faster, more efficient analyses can be attained with greater sensitivity and reduced system pressure by switching from FPP to SPP—without altering method conditions.

The Effects of LC Particle Choice on Column Performance: 2.7 vs. 5 µm Diameter Superficially Porous Particles (SPP)

Restek's Raptor™ LC columns feature superficially porous particles (commonly referred to as SPP or “core-shell” particles) and are available in both 2.7 and 5 µm particle sizes, giving analysts greater flexibility. However, it may not always be clear which particle size to choose. In this technical note, we will examine the differences in efficiency, sensitivity, and pressure between Raptor™ LC columns packed with 2.7 vs. 5 µm particles and provide advice on making the appropriate LC particle choice

Featured Application: Multiclass Veterinary Antibiotics on Raptor™ C18 by LC-MS/MS - One Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics

One Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics: The use of antibiotics on food-producing animals is a public health and safety concern due to the potential of generating drug-resistant bacteria. A sensitive, efficient, and reliable analytical method for different classes of antibiotics is necessary to meet regulations covering maximum residue limits (MRL) for a variety of animal tissue and food products. The Raptor™ C18 LC column is the ideal choice.

Featured Application: 231 pain management and drugs of abuse compounds in under 10 minutes by LC-MS/MS - Big Pain Assays Aren't a Big Pain with the Raptor™ Biphenyl LC Column

The use of pain management drugs is steadily increasing. As a result, hospital and reference labs are seeing an increase in patient samples that must be screened for a wide variety of pain management drugs to prevent drug abuse and to ensure patient safety and adherence to their medication regimen. Restek's "Big Pain" LC-MS/MS methodology features a 231-compound multiclass drug and metabolite screen that can be performed in under 10 minutes with just one column: The Raptor™ Biphenyl.

Featured Application: LC Analysis of Vitamin D Epimers on Raptor FluoroPhenyl Columns - Fast, Accurate LC-MS/MS Analysis of the C3 Epimer of 25-Hydroxyvitamin D on Raptor FluoroPhenyl Columns

Vitamin D2 and D3 25-hydroxy metabolites are important diagnostic markers for vitamin D status, but these compounds cannot be distinguished from their epimeric forms on a C18 column. The Raptor FluoroPhenyl column provides ample chromatographic resolution for these isobaric epimers, which possess unequal bioactivities, so accurate results are generated and thus the proper diagnosis can be made.

Featured Application: LC Analysis of 4-MEI on Raptor FluoroPhenyl Columns - Reliable HILIC LC-MS/MS Analysis of 4-Methylimidazole (4-MEI) on Raptor FluoroPhenyl Columns

Raptor FluoroPhenyl columns perform well in both HILIC and reversed phase modes, giving method developers the flexibility select the mode that performs best for their specific compounds of interest. Here we use a Raptor Fluorophenyl column in HILIC mode for LC analysis of 4-methylimidazole (4-MEI). This approach provides more retention than reversed phase mode and can be applied to determining 4-MEI levels in caramel colorings to monitor formulations and also in foods and beverages to assess potential exposure.

Raptor Biphenyl LC Columns Brochure

Raptor LC columns combine the speed of superficially porous particles (i.e., SPP or “core-shell”) with the resolution of highly selective USLC technology. Featuring Restek's most popular LC stationary phase, the rugged Raptor Biphenyl is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 6MB)

Dissecting Raptor LC Columns: A closer look at a new species

When we engineered our superficially porous particle (SPP or "core-shell") Raptor LC columns, we developed the bonding chemistries that are best suited to both the SPP construction and our highly selective USLC phases. But we didn't stop here. Take a closer look at a new species as we dissect the upgraded hardware and new, proprietary packing techniques behind Raptor LC columns and Raptor EXP guard columns. (PDF - 2MB)

Raptor ARC-18 LC Columns Brochure

Raptor LC columns combine the speed of superficially porous particles (i.e., SPP or “core-shell”) with the resolution of highly selective USLC technology. The sterically protected ARC-18 phase was designed and intended specifically for use with acidic mobile phases on LC-MS/MS systems. Even after extended use in low-pH (≤ 2.0) conditions, it offers consistent and well-balanced retention, peak shape, and response. Choose Raptor ARC-18 columns for the rapid analysis of large, multiclass assays by mass spectrometry. (PDF - 6MB)

Raptor™ SPP C18 Columns Sales Sheet

Every LC lab has a cache of C18s, but every C18 is not created equal. When you need a general-purpose LC column, don't just grab any C18. Choose the speed, efficiency, and long-lasting ruggedness of the new Raptor™ C18. (PDF - 2MB)

Featured Application: Multiclass Veterinary Antibiotics on Raptor™ C18 by LC-MS/MS

One Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics: The use of antibiotics on food-producing animals is a public health and safety concern due to the potential of generating drug-resistant bacteria. A sensitive, efficient, and reliable analytical method for different classes of antibiotics is necessary to meet regulations covering maximum residue limits (MRL) for a variety of animal tissue and food products. The Raptor™ C18 LC column is the ideal choice. (PDF - 1MB)

Raptor™ C18 LC Columns Brochure

Raptor™ Speed, Efficiency, and Ruggedness—in C18: Every LC lab has a cache of C18s, but every C18 is not created equal. When you need a general-purpose LC column, don’t just grab any C18. Choose the speed, efficiency, and long-lasting ruggedness of the Raptor™ C18 (PDF - 2MB)

Raptor™ ARC-18 LC Columns Sales Sheet

Ahead of the Curve for Large, Multiclass Lists by Mass Spec: Designed and intended specifically for use on LC-MS/MS systems, the Raptor™ ARC-18 SPP LC column offers a well-balanced retention profile without the drawbacks of using an ordinary C18 in the harsh, acidic mobile phases needed for mass spectrometry (MS). (PDF - 2MB)

Raptor™ Biphenyl LC Columns Sales Sheet

Fast, Rugged Raptor™ Columns with Time-Tested Selectivity: The industry-leading Biphenyl, first introduced by Restek in 2005, is our most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, the rugged Raptor™ Biphenyl column is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). (PDF - 4MB)

Raptor™ FluoroPhenyl LC Columns Sales Sheet

Restek's Raptor™ FluoroPhenyl phase gives you the flexibility to run in reversed-phase or HILIC modes so you can use the mode that is best for your target analytes. The Restek® Raptor™ FluoroPhenyl column is also amenable to LC-MS because it is extremely reliable and efficient with acidic mobile phases. (PDF - 2MB)

Raptor™ FluoroPhenyl LC Columns Brochure

Restek has now extended the speed and reliability of Raptor™ column technology into the HILIC realm with the addition of Raptor™ FluoroPhenyl columns. Restek's Raptor™ FluoroPhenyl phase offers chromatographers the ability to run in reversed-phase or HILIC mode for a variety of compounds. The Restek® Raptor™ FluoroPhenyl column is also amenable to LC-MS because it is extremely reliable and efficient with acidic mobile phases. (PDF - 3MB)

Infographic: Judging Raptor SPP Core-Shell Particles—2.7 vs. 5 µm Particle Sizes

Restek's Raptor™ superficially porous particle (SPP) LC columns are available in both 2.7 and 5 µm diameter particle sizes. This selection gives you greater flexibility to tailor your column to the task at hand—and our infographic takes the deliberation out of choosing the most appropriate size for your specific assay! (PDF - 3MB)

Raptor HILIC-Si: Simplify the Switch to HILIC

The Raptor HILIC-Si column simplifies the switch to HILIC because it delivers rugged Raptor performance, provides SPP column speed for faster analyses, ensures retention of polar compounds without ion pairing reagents, and is fully reliable, efficient, and selective with LC-MS compatible mobile phases. (PDF - 2MB)

Definitive EtG/EtS LC-MS/MS Analysis: A Rugged 4-min Method for High-Throughput Labs

Methods for monitoring alcohol consumption biomarkers EtG and EtS are generally limited by poor retention and coelution with matrix interferences, as well as by long analysis times and short column lifetimes. The dilute-and-shoot EtG/EtS LC-MS/MS analysis developed here using the novel Raptor EtG/EtS column easily resolves EtG and EtS from matrix interferences, providing consistent, accurate results for high-throughput labs testing human urine samples for alcohol consumption. (PDF - 1MB)

All Matrix Interferences Resolved

Typical methods for EtG and EtS analysis have several limitations: poor resolution from matrix components, long run times that limit sample throughput, and short column lifetimes. Restek’s new Raptor EtG/EtS column consistently retains and resolves EtG and EtS from matrix interferences, providing fast, accurate, and reliable results injection after injection. (PDF - 2MB)