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Analysis of Taxanes on Raptor FluoroPhenyl by LC-MS/MS

PeakstR (min)Precursor IonProduct Ion 1Product Ion 2
1.Baccatin III0.97587.0405.1105.0
2.Docetaxel1.25808.1527.3226.1
3.Paclitaxel1.33854.1569.3286.2
image
LC_BA0351
ColumnRaptor FluoroPhenyl (cat.# 931955E)
Dimensions:50 mm x 3 mm ID
Particle Size:5 µm
Temp.:35 °C
Sample
Diluent:Water
Conc.:100 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.87525
2.000.8595
2.010.87525
3.500.87525
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
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Taxanes

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paclitaxel

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docetaxel

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baccatin

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III

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