Fentanyl, Metabolite, and Analogues in Urine on Raptor™ Biphenyl by LC-MS/MS

PeakstR (min)Conc.
(ng/mL)
Q1Q3 QuantifierQ3 Qualifier
1.Norfentanyl1.0410233.1084.08150.11
2.Norfentanyl-d5 (IS)1.021238.1084.08-
3.Acetyl fentanyl1.4810323.29188.18105.10
4.Acetyl fentanyl-13C61.481329.22188.22-
5.Fentanyl1.6510337.29188.22105.09
6.Fentanyl-d5 (IS)1.641342.35188.22-
7.Sufentanil1.8410387.35238.18111.07
8.Sufentanil-d5 (IS)1.831392.29238.18-
image
LC_CF0591
ColumnRaptor ™ Biphenyl (cat.# 9309552)
Dimensions:50 mm x 2.1 mm ID
Particle Size:5 µm
Temp.:30 °C
Sample
Conc.: A 10 ng/mL standard was prepared in urine. The internal standard was fortified to a final concentration of 1 ng/mL. An 80 μL aliquot of the 10 ng/mL urine standard was diluted with 320 μL of 70:30 water:methanol solution (5-fold dilution) prior to analysis.
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.47030
2.000.42080
2.010.47030
3.500.47030
DetectorMS/MS
Ion Mode:ESI+
InstrumentHPLC
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LC-MS/MS

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