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Nicotine-Related Compounds in Urine on Raptor™ Biphenyl by LC-MS/MS
|Peaks||tR (min)||Precursor Ion||Product Ion|
|Column||Raptor™ Biphenyl (cat.# 9309512)|
|Dimensions:||100 mm x 2.1 mm ID|
|Particle Size:||5 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor™ Biphenyl EXP® cartridges 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252)|
|Conc.:||25 ng/mL (fortified human urine)|
|Inj. Vol.:||5 µL|
|A:||0.1% Formic acid, 5 mM ammonium formate in water|
|B:||0.1% Formic acid in methanol|
|Notes||The urine from a non-tobacco user was fortified with analytes to a final concentration of 25 ng/mL and prepared by a liquid-liquid extraction method. A 250 μL urine sample was mixed with 50 μL of 5N NaOH and extracted with 1.5 mL of 50/50 methylene chloride/diethyl ether. A portion of organic phase (1.0 mL) was dried and reconstituted with 200 μL of water for analysis.|
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