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Barbiturate Drug Panel on Raptor Biphenyl by LC-MS/MS

PeakstR (min)Precursor IonProduct IonProduct Ion
1.Phenobarbital1.97230.9187.885.0
2.Butalbital2.21223.0180.084.9
3.Amobarbital2.95225.0182.084.8
4.Pentobarbital3.04225.0182.084.9
5.Secobarbital3.69237.0193.984.8
image
LC_CF0618
ColumnRaptor Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:30 °C
Sample
Diluent:Water
Conc.:500 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:Water + 0.1% formic acid
B:Methanol + 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.65545
4.000.64555
4.010.65545
6.000.65545
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentUHPLC
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