25-Hydroxyvitamin D2/D3 and 3-Epi-25-Hydroxyvitamin D2/D3 on Raptor™ FluoroPhenyl by LC-MS/MS

image
LC_CF0644
ColumnRaptor FluoroPhenyl (cat.# 9319A1E)
Dimensions:100 mm x 3 mm ID
Particle Size:2.7 µm
Temp.:30 °C
Sample
Diluent:Water:methanol (50:50)
Conc.:25-50 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid in water
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.62575
4.000.61585
4.100.60100
5.000.60100
5.010.62575
7.000.62575
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentHPLC
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