Immunosuppressive Drugs in Blood on Raptor Biphenyl by LC-MS/MS
|Precursor Ion||Product Ion|
|Column||Raptor Biphenyl (cat.# 9309A52)|
|Dimensions:||50 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)|
|Inj. Vol.:||5 µL|
|A:||0.05% Formic acid, 5 mM ammonium formate in water|
|Max Pressure:||305 bar|
|Notes||Sample Preparation Procedure:|
Human whole blood was fortified with 4 immunosuppressive drugs at 10 ng/mL. For quantitation, cyclosporin D was used as the internal standard for cyclosporin A and ascomycin was used as the internal standard for tacrolimus, sirolimus, and everolimus. The blood sample (100 μL) was mixed with 200 μL of precipitation solution (1:4 v/v 0.2 M ZnSO4:methanol) containing 50 ng/mL of cyclosporin D and 5 ng/mL of ascomycin. The mixture was vortexed for 20 seconds at 3,000 rpm and then centrifuged for 10 minutes at 4,300 rpm. The supernatant was directly injected (5 μL) for analysis.
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