HPLC & UHPLC Analytical Columns

• Ideal for high-throughput LC-MS/MS applications with minimal sample preparation.
• Well-balanced retention profile for better detection and integration of large, multiclass analyte lists.
• Sterically protected to endure low-pH mobile phases without sacrificing retention or peak quality.
• Part of Restek’s Raptor LC column line featuring 1.8, 2.7, and 5 µm SPP core-shell silica.

• A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography.
• Wide pH range (2–8) provides excellent data quality for many applications, matrices, and compounds.
• Offers the highest hydrophobic retention of any Raptor phase.
• Part of Restek’s Raptor LC column line featuring 1.8, 2.7, and 5 µm SPP core-shell silica.

• Ideal for bioanalytical testing applications like drug and metabolite analyses.
• Heightened selectivity and retention for compounds that are hard to resolve or elute early on C18 and other phenyl chemistries.
• Limits ionization suppression and allows simple, MS-friendly mobile phases.
• Part of Restek’s Raptor LC column line featuring 1.8, 2.7, and 5 µm SPP core-shell silica.

• Capable of both reversed-phase and HILIC separations.
• Ideal for increasing sensitivity and selectivity in LC-MS analyses.
• Offers increased retention for charged bases.
• Part of Restek’s Raptor LC column line featuring 1.8, 2.7, and 5 µm SPP core-shell silica.
• Switch to a Raptor FluoroPhenyl LC column when you need more retention and selectivity for basic and hydrophilic compounds than you can achieve on a C18.

• HILIC silica column designed for increased retention of polar compounds without ion-pairing reagents.
• 2.7 µm Raptor core-shell particles provide the speed of SPP, with greater efficiency and capacity than 5 µm particles.
• Ideal for increasing sensitivity and selectivity in LC-MS analyses.
• Fully compatible with both HPLC and UHPLC when using low back-pressure HILIC mobile phases.
• Switch to a Raptor HILIC-Si LC column when you need more retention and resolution of hydrophilic, polar analytes.

• Capable of both reversed-phase and HILIC separations.
• Ideal for increasing sensitivity and selectivity in LC-MS analyses.
• Offers increased retention for charged bases.

• A traditional end-capped C18 ideal for general-purpose use in reversed-phase chromatography.
• Wide pH range (2–8) provides excellent data quality for many applications, matrices, and compounds.
• Offers high hydrophobic retention.

• Ideal for bioanalytical testing applications like drug and metabolite analyses.
• Heightened selectivity and retention for compounds that are hard to resolve or elute early on C18 and other phenyl chemistries.
• Limits ionization suppression and allows simple, MS-friendly mobile phases.
• Restek’s most popular LC stationary phase.

Stationary Phase Category: phenyl (L11)
Ligand Type: unique Biphenyl
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 15%
End-Cap: yes
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: phenyl (L11)
Ligand Type: unique Biphenyl
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 8%
End-Cap: yes
Surface Area: 150 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 5 µm, spherical
pH Range: 2.5 to 8
Maximum Temperature: 80 °C
Maximum Pressure: 1,034 bar/15,000 psi

• Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
• Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
• Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
• Ideal for a wide range of applications from food to pharma;
  5 phases cover 98% of USP methods.

• Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
• Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
• Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
• Ideal for a wide range of applications from food to pharma;
  5 phases cover 98% of USP methods.

• Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
• Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
• Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
• Ideal for a wide range of applications from food to pharma;
  5 phases cover 98% of USP methods.

• Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
• Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
• Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
• Ideal for a wide range of applications from food to pharma;
  5 phases cover 98% of USP methods.

• Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
• Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
• Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
• Ideal for a wide range of applications from food to pharma;
  5 phases cover 98% of USP methods.

Stationary Phase Category: polar-embedded alkyl (L68)
Ligand Type: proprietary polar functional embedded alkyl
Particle: 1.9 µm, spherical
Pore Size: 140 Å
Carbon Load: proprietary
End-Cap: no
Surface Area: 150 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 1.9 µm, spherical
Pore Size: 140 Å
Carbon Load: proprietary
End-Cap: no
Surface Area: 150 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: fluorophenyl propyl (L43)
Ligand Type: pentafluorophenyl propyl
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 6%
End-Cap: yes
Surface Area: 150 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: phenyl (L11)
Ligand Type: Biphenyl
Particle: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 7%
End-Cap: yes
Surface Area: 100 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: fluorophenyl propyl (L43)
Ligand Type: pentafluorophenyl propyl
Particle: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 5%
End-Cap: yes
Surface Area: 100 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: C4, butylsilane (L26)
Ligand Type: monomeric C4
Particle: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 3.5%
End-Cap: yes
Surface Area: 100 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: C8, octylsilane (L7)
Ligand Type: monomeric C8
Particle: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 5%
End-Cap: yes
Surface Area: 100 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: C18, octadecylsilane (L1)
Ligand Type: monomeric C18
Particle: 3 µm or 5 µm, spherical
Pore Size: 300 Å
Carbon Load: 9%
End-Cap: yes
Surface Area: 100 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: bare silica (L3)
Ligand Type: none
Particle: 5 µm, spherical
Pore Size: 300 Å
Carbon Load: n/a
End-Cap: n/a
Surface Area: 100 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: bare silica (L3)
Ligand Type: none
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: n/a
End-Cap: n/a
Surface Area: 150 m²/g
pH Range: 2.5 to 8.0
Maximum Temperature: 80 °C

Stationary Phase Category: cyano (L10)
Ligand Type: cyanopropylsilane
Particle: 1.9 µm or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 4%
End-Cap: yes
Surface Area: 150 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: C8, octylsilane (L7)
Ligand Type: monomeric C8
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 6%
End-Cap: yes
Surface Area: 150 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: C18, octadecylsilane (L1)
Ligand Type: monomeric C18
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 11%
End-Cap: yes
Surface Area: 150 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 5 µm, spherical
Pore Size: 60 Å
Carbon Load: n/a
End-cap: n/a
Surface Area: 450 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Highly retentive phase for normal phase separations. Very high surface area, Type B silica packing.

Ideal for MOSH/MOAH analysis by EN 16995. For compatibility with Axel Semrau and other automated systems, add the Level 3 Trident column protection system and Allure Silica guard cartridge listed in Related Products.

• Proven performance for accurate, reliable ethyl glucuronide (EtG) and ethyl sulfate (EtS) analysis.
• Strong retention consistently resolves analytes from matrix interferences.
• Long column lifetime ensures consistent performance injection after injection.
• Fast, 4-minute, dilute-and-shoot LC-MS/MS analysis supports high sample throughput.

• Ultra Selective Liquid Chromatography (USLC) method development toolbox contains all four USLC stationary phases in one convenient package.
• Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm lengths.

Stationary Phase Category: phenyl (L11)
Ligand Type: proprietary phenyl ligand
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 17%
End-Cap: yes
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: fluorophenyl propyl (L43)
Ligand Type: pentafluorophenyl propyl
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 11%
End-Cap: yes
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 4 µm, spherical
Pore Size: 110 Å
Carbon Load: proprietary
End-Cap: no
Surface Area: 180 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 5 µm, spherical
Pore Size: 60 Å
Carbon Load: proprietary
End-Cap: yes
Surface Area: 450 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: proprietary
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 5 µm, spherical
Pore Size: 60 Å
Carbon Load: proprietary
End-Cap: no
Surface Area: 450 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: proprietary
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: bare silica (L3)
Ligand Type: none
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: n/a
End-Cap: n/a
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: polar-embedded alkyl (L68)
Ligand Type: proprietary polar functional embedded alkyl
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 12%
End-Cap: no
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: cyano (L10)
Ligand Type: cyanopropylsilane
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 8%
End-Cap: yes
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: modified C18 (L1)
Ligand Type: proprietary polar modified and functionally bonded C18
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 15%
End-Cap: no
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: C18, octadecylsilane (L1)
Ligand Type: monomeric C18
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 20%
End-Cap: yes
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: C8, octylsilane (L7)
Ligand Type: monomeric C8
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 12%
End-Cap: yes
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: C4, butylsilane (L26)
Ligand Type: monomeric C4
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 9%
End-Cap: yes
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: trimethylsilane (L13)
Ligand Type: monomeric C1
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 5%
End-Cap: yes
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Stationary Phase Category: amino (L8)
Ligand Type: aminopropylsilane
Particle: 3 µm or 5 µm, spherical
Pore Size: 100 Å
Carbon Load: 2%
End-Cap: no
Surface Area: 300 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Particle: 5 µm, spherical
Pore Size: 60 Å
Carbon Load: n/a
End-cap: n/a
Surface Area: 450 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C

Highly retentive phase for normal phase separations. Very high surface area, Type B silica packing.

Stationary Phase Category: modified C18 (L1)
Ligand Type: proprietary polar modified and functionally bonded C18
Particle: 1.9 µm, 3 µm, or 5 µm, spherical
Pore Size: 140 Å
Carbon Load: 6%
End-Cap: no
Surface Area: 150 m²/g
pH Range: 2.5 to 8
Maximum Temperature: 80 °C