Chlorinated Pesticide Residues in Olive Oil Rtx-CLPesticides2

	Peaks	Quant. ion	Qual. ion 1	Qual. ion 2
1.	α-BHC*	219	181	109
2.	γ-BHC	219	181	109
3.	β-BHC	219	181	109
4.	δ-BHC	219	181	109
5.	Heptachlor	272	237	100
6.	Aldrin	263	293	220
7.	Heptachlor epoxide	263	237	81
8.	δ-Chlordane	272	237	65
9.	α-Chlordane	272	237	65
10.	Endosulfan I	195	207	241
11.	4,4'-DDE	246	318	176
12.	Dieldrin	79	263	277
13.	Endrin	263	281	81
14.	4,4'-DDD	235	165	199
15.	Endosulfan II	195	207	-
16.	4,4'-DDT	235	165	199
17.	Endrin aldehyde	67	250	345
18.	Endosulfan sulfate	272	229	239
19.	Methoxychlor	227	274	-
20.	Endrin ketone	67	317	281

* α-BHC not detected due to low recovery during sample preparation.

GC_FF01043

Column	Rtx-CLPesticides2, 30 m, 0.25 mm ID, 0.20 µm (cat.# 11323)
Sample	Olive oil spiked with Organochlorine Pesticide Mix AB # 3 (cat.# 32415)
Conc.:	10 µg/mL
Injection
Inj. Vol.:	1 µL splitless (hold 0.5 min)
Liner:	Single Taper w/Wool (cat.# 22286-200.1)
Inj. Temp.:	225 °C
Oven
Oven Temp.:	140 °C (hold 0.5 min) to 268 °C at 20 °C/min to 290 °C at 3 °C/min to 330 °C at 20 °C/min (hold 5 min)
Carrier Gas	He, constant flow
Flow Rate:	1 mL/min
Detector	MS
Mode:	SIM
Transfer Line Temp.:	320 °C
Ionization Mode:	EI
Notes	Extraction and dSPE Cleanup for Pesticide Residues in Olive Oil Test sample: A 1.5 mL sample of commercially obtained virgin olive oil was spiked with a standard organochlorine pesticide mix. The spiked sample was processed as follows: 1. Dilute with 1.5 mL hexane. 2. Add 6 mL of acetonitrile (ACN). 3. Mix for 30 minutes on a shaker. 4. Allow layers to separate (approximately 20 minutes), then collect the top (ACN) layer. 5. Repeat the liquid-liquid extraction (steps 2–4) and combine both ACN extract layers. 6. Place 1 mL of the combined ACN extract in a 1.5 mL tube containing 150 mg magnesium sulfate and 50 mg PSA. 7. Shake the tube for 2 minutes. 8. Centrifuge at 3,000 U/min for approximately 5 minutes. 9. Remove the top layer and inject directly into the gas chromatograph system.

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olive oil

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CLP

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chlorinated pesticides

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