QuEChERS Extract of Cannabis on Rxi-17Sil MS and Rxi-5ms by GCxGC-TOFMS

GC_FF1207

Column	Rxi-17Sil MS 30 m, 0.25 mm ID, 0.25 µm (cat.# 14123)
	Rxi-5ms 0.9 m, 0.18 mm ID, 0.18 µm,
Injection
Inj. Vol.:	1 µL split (split ratio 20:1)
Liner:	Premium 4 mm Precision Inlet Liner w/Wool (cat.# 23305.1)
Inj. Temp.:	275 °C
Oven
Oven Temp.:	Rxi-17Sil MS: 40 °C (hold 1 min) to 320 °C at 5 °C/min (hold 3 min) Rxi-5ms: 45 °C (hold 1 min) to 325 °C at 5 °C/min (hold 3 min)
Carrier Gas	He, corrected constant flow (2 mL/min)
Modulation
Modulator Temp. Offset:	20 °C
Second Dimension Separation Time:	2 sec
Hot Pulse Time:	0.6 sec
Cool Time between Stages:	0.4 sec
Detector	TOFMS
Transfer Line Temp.:	310 °C
Analyzer Type:	TOF
Source Temp.:	250 °C
Electron Energy:	70 eV
Mass Defect:	-20 mu/100 u
Solvent Delay Time:	4.5 min
Tune Type:	PFTBA
Ionization Mode:	EI
Acquisition Range:	45-500 amu
Spectral Acquisition Rate:	200 spectra/sec
Instrument	LECO Pegasus 4D GCxGC-TOFMS
Notes	Sample Preparation: Sample: Up to 2 g of finely ground cannabis in a 50 mL polypropylene centrifuge tube. Extraction: Add 10 mL acetonitrile and 10 mL of organic-free water, then shake thoroughly. Soak 1 hour, then aggressively vortex 30 minutes. Add Q-sep packet (cat.# 26235), shake 1 minute, then centrifuge 5 minutes at 3,000 g with Q-sep 3000 centrifuge (cat.# 26230). NOTE: See GC_FF1208 and GC_FF1209 to zoom in on terpenes and cannabinoids regions.

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