Nutritional Labeling FAMEs on Rt-2560 by AOCS Method Ce-1j-07

PeakstR (min)Conc.
Structural Nomenclature
1.Methyl caproate7.5240C6:0
2.Methyl octanoate7.8840C8:0
3.Methyl decanoate8.4840C10:0
4.Methyl undecanoate8.9320C11:0
5.Methyl dodecanoate9.5140C12:0
6.Methyl tridecanoate10.2720C13:0
7.Methyl myristate11.2740C14:0
8.Methyl myristoleate12.4820C14:1 (c9)
9.Methyl pentadecanoate12.5720C15:0
10.Methyl pentadecenoate14.1520C15:1 (C10)
11.Methyl palmitate14.2860C16:0
12.Methyl palmitoleate15.9820C16:1 (c9)
13.Methyl heptadecanoate16.5120C17:0
14.Methyl heptadecenoate18.6820C17:1 (c10)
15.Methyl stearate19.4340C18:0
16.Methyl octadecenoate21.0820C18:1 (t9)
17.Methyl oleate21.8540C18:1 (c9)
18.Methyl linolelaidate24.0920C18:2 (t9,t12)
19.Methyl linoleate26.1420C18:2 (c9,c12)
20.Methyl arachidate28.2540C20:0
21.Methyl linolenate29.9820C18:3 (c6,c9,c12)
22.Methyl eicosenoate32.0520C20:1 (c11)
23.Methyl linolenate32.4120C18:3 (c9,c12,c15)
24.Methyl heneicosanoate33.6620C21:0
25.Methyl eicosadienoate35.3320C20:2 (c11,c14)
26.Methyl behenate36.6440C22:0
27.Methyl eicosatrienoate37.4420C20:3 (c8,c11,c14)
28.Methyl erucate38.5120C22:1 (c13)
29.Methyl eicosatrienoate38.7220C20:3 (c11,c14,c17)
30.Methyl arachidonate39.1220C20:4 (c5,c8,c11,c14,c17)
31.Methyl tricosanoate39.7420C23:0
32.Methyl docosadienoate41.6420C22:2 (c13,c16)
33.Methyl eicosapentaenoate43.0720C20:5 (c5,c8,c11,c14,c17)
34.Methyl lignocerate43.1140C24:0
35.Methyl nervonate45.3320C24:1 (c15)
36.Methyl docosahexaenoate54.0220C22:6 (c4,c7,c10,c13,c16,c19)
ColumnRt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
SampleFood industry FAME mix (cat.# 35077)
Conc.:1,000 µg/mL
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Premium 4 mm Precision liner w/wool (cat.# 23305.1)
Inj. Temp.:235 °C
Oven Temp.:180 °C (hold 32 min) to 215 °C at 20 °C/min (hold 31.25 min)
Carrier GasHe, constant flow
Flow Rate:2.0 mL/min
DetectorFID @ 325 °C
Make-up Gas Flow Rate:45 mL/min
Make-up Gas Type:N2
Hydrogen flow:30 mL/min
Air flow:300 mL/min
Data Rate:20 Hz
InstrumentAgilent 7890A GC
NotesC4:0 Methyl butyrate (623-42-7) elutes in the solvent front.
Download PDF


Fats and Oils


AOCS Ce-1j-07


Nutritional Labeling


Trans Fats


Fatty Acid Profiling




High Resolution GLC

Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.

Restek Domestic Customer Service



Your Full Name

Your Email

Company Name


Spam Block (Please leave this blank)

all fields required

Thank you

Your message has been sent. We will be in touch shortly.

Message not sent

Sorry, your message could not be sent at this time. Please try again later, or contact Restek or your local Restek representative via phone.