Cholesterol and 5α-Cholestane on DiatoSorb-WHP Packed Column
| Peaks | |
|---|---|
| 1. | 5α-Cholestane |
| 2. | Cholesterol |
GC_PC1300
| Column | 3% OV-101, 100/120 mesh on DiatoSorb-WHP, SilcoSmooth tubing, 1 m, 1/8 in OD, 2 mm ID |
| Sample | Cholesterol, 5α-cholestane |
| Diluent: | Chloroform |
| Conc.: | 1,000 µg/mL |
| Injection | |
| Inj. Vol.: | 1.0 µL on-column |
| Inj. Temp.: | 220 °C |
| Oven | |
| Oven Temp.: | 250 °C (hold 8 min) |
| Carrier Gas | He, constant flow |
| Flow Rate: | 40 mL/min |
| Detector | FID @ 260 °C |
| Instrument | Agilent 7890B GC |
| Notes | Column inertness results in symmetrical peak shape and almost no adsorption of active compounds. |
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Restek Corporation, U.S., 110 Benner Circle, Bellefonte, PA 16823
Copyright © 2019 Restek Corporation. All rights reserved.
Copyright © 2019 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.