Cholesterol and 5α-Cholestane on DiatoSorb-WHP Packed Column

	Peaks
1.	5α-Cholestane
2.	Cholesterol

GC_PC1300

Column	3% OV-101, 100/120 mesh on DiatoSorb-WHP, SilcoSmooth tubing, 1 m, 1/8 in OD, 2 mm ID
Sample	Cholesterol, 5α-cholestane
Diluent:	Chloroform
Conc.:	1,000 µg/mL
Injection
Inj. Vol.:	1.0 µL on-column
Inj. Temp.:	220 °C
Oven
Oven Temp.:	250 °C (hold 8 min)
Carrier Gas	He, constant flow
Flow Rate:	40 mL/min
Detector	FID @ 260 °C
Instrument	Agilent 7890B GC
Notes	Column inertness results in symmetrical peak shape and almost no adsorption of active compounds.

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