Cholesterol and 5α-Cholestane on DiatoSorb-WHP Packed Column
Peaks | |
---|---|
1. | 5α-Cholestane![]() |
2. | Cholesterol![]() |

GC_PC1300
Column | 3% OV-101, 100/120 mesh on DiatoSorb-WHP, SilcoSmooth tubing, 1 m, 1/8 in OD, 2 mm ID |
Sample | Cholesterol, 5α-cholestane |
Diluent: | Chloroform |
Conc.: | 1,000 µg/mL |
Injection | |
Inj. Vol.: | 1.0 µL on-column |
Inj. Temp.: | 220 °C |
Oven | |
Oven Temp.: | 250 °C (hold 8 min) |
Carrier Gas | He, constant flow |
Flow Rate: | 40 mL/min |
Detector | FID @ 260 °C |
Instrument | Agilent 7890B GC |
Notes | Column inertness results in symmetrical peak shape and almost no adsorption of active compounds. |
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