High Speed GC-MS of Carcinogenic Aryl Amines Resulting From Reductive Cleavage of Azo Dyes on Rxi-35Sil MS (Scan Mode)

	Peaks	tR (min)	Conc. (µg/mL)	Molecular Ion (m/z)
1.	o-Toluidine	1.14	10	107
2.	o-Anisidine	1.56	10	123
3.	4-Chloroaniline	1.71	10	127
4.	p-Cresidine	1.95	10	137
5.	2,4,5-Trimethylaniline	1.97	10	135
6.	3-Chloro-o-toluidine	2.08	10	141
7.	4-Chloro-o-toluidine	2.13	10	141
8.	2,4-Diaminotoluene	2.70	10	122
9.	2,4,5-Trichloroaniline (IS)	3.25	30	197
10.	2-Naphthylamine	3.46	10	143
11.	2-Aminobiphenyl	3.50	10	169
12.	2-Amino-4-nitrotoluene	3.73	10	152
13.	4-Aminobiphenyl	4.31	10	169
14.	p-Aminoazobenzene	5.66	10	197
15.	4,4'-Oxydianiline	5.90	10	200
16.	4,4'-Diaminodiphenylmethane	5.94	10	198
17.	Benzidine	5.96	10	184
18.	o-Aminoazotoluene	6.23	10	225
19.	3,3'-Dimethyl-4,4'-diaminodiphenylmethane	6.48	10	226
20.	3,3'-Dimethylbenzidine	6.57	10	212
21.	4,4’-Thiodianiline	6.92	10	216
22.	3,3'-Dichlorobenzidine	7.05	10	252
23.	4,4'-Methylenebis(2-chloroaniline)	7.05	10	266
24.	3,3'-Dimethoxybenzidine	7.09	10	244

Elution order of positional isomers was verified with individual standards.

GC_QA0096

Column	Rxi-35Sil MS, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13820)
Sample	AccuStandard carcinogenic aryl amine mix (cat.# AE-000-49-R1)
Diluent:	Ethyl acetate
Conc.:	10 µg/mL
Injection
Inj. Vol.:	1 µL split (split ratio 10:1)
Liner:	Premium 4 mm Precision liner w/wool (cat.# 23305.1)
Inj. Temp.:	275 °C
Oven
Oven Temp.:	100 °C to 320 °C at 27 °C/min (hold 1.75 min)
Carrier Gas	He, constant flow
Flow Rate:	2.0 mL/min
Detector	MS
Mode:	Scan
Scan Program:	Group Start Time (min) Scan Range (amu) Scan Rate (scans/sec) 1 2.0 30-300 5.5
Transfer Line Temp.:	330 °C
Analyzer Type:	Quadrupole
Source Type:	Inert
Drawout Plate:	3 mm ID
Source Temp.:	250 °C
Quad Temp.:	180 °C
Electron Energy:	70 eV
Solvent Delay Time:	2.0 min
Tune Type:	PFTBA
Ionization Mode:	EI
Instrument	Agilent 7890A GC & 5975C MSD

Download PDF

RELATED CHROMATOGRAM LIBRARY SEARCHES

high speed GC-MS

,

high speed GC

,

fast GC

,

azo dye

,

carcinogenic amines

,

Rxi-35Sil MS

,

textiles

,

regulated azo dyes

,

aryl amines

Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.