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Combined Analysis of Synthetic Cannabinoids & Metabolites in Urine by LC-MS/MS on Raptor Biphenyl

PeakstR (min)Precursor IonQuantifier Product Ion Qualifier Product Ion
1.Pravadoline2.15379.29135.04114.16
2.AM22332.44459.25112.298.15
3.JWH-200-d52.47390.34155.07NA
4.JWH-2002.48385.28155.07114.16
5.WIN 55, 2123.34427.29155.07127.14
6.JWH-073 N-butanoic acid3.39358.27155.08127.11
7.JWH-073 4-hydroxybutyl3.4344.24155.09127.09
8.JWH-018 N-pentanoic acid3.49372.18155.08127.14
9.JWH-018 5-hydroxypentyl-d53.54363.5155.08NA
10.JWH-018 5-hydroxypentyl3.55358.27155.08127.11
11.JWH-073 6-hydroxyindole3.77344.24155.09127.09
12.JWH-073 5-hydroxyindole-d73.81351.21155.07NA
13.JWH-073 5-hydroxyindole3.83344.24155.09127.09
14.JWH-073 7-hydroxyindole3.92344.24155.09127.09
15.JWH-018 6-hydroxyindole3.94358.27155.08127.11
16.JWH-018 5-hydroxyindole3.99358.27155.08127.11
17.JWH-018 7-hydroxyindole4.08358.27155.08127.11
18.RCS-44.15322.27135.1277.09
19.XLR-114.21330.25232.17125.1
20.JWH-015-d74.27335.28155.07NA
21.JWH-2504.27336.28121.1291.07
22.JWH-0154.29328.26155.07127.13
23.AM22014.30360.26155.07127.14
24.JWH-2034.39340.23188.18125.09
25.JWH-0734.42328.26155.07127.13
26.UR-1444.44312.32214.17125.1
27.JWH-073 4-hydroxyindole4.53344.24155.09127.09
28.JWH-018-d94.55351.34155.07NA
29.JWH-0184.57342.27155.08127.11
30.JWH-0814.64372.28185.12157.09
31.JWH-018 4-hydroxyindole4.66358.27155.08127.11
32.JWH-1224.69356.29169.12141.11
33.JWH-0194.70356.29155.07127.1
34.JWH-2104.84370.31183.12153.26
image
LC_CF0613
ColumnRaptor Biphenyl (cat.# 9309A5E)
Dimensions:50 mm x 3.0 mm ID
Particle Size:2.7 µm
Guard Column:Raptor Biphenyl EXP guard column cartridge 5.0 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)
Sample
Conc.:5 ng/mL standard was prepared in urine and diluted 3x with 50:50 methanol:water
Inj. Vol.:2 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.67525
1.000.67525
5.000.6595
5.500.6595
5.510.67525
7.000.67525
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
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