25-Hydroxyvitamin D2/D3 and 3-Epi-25-Hydroxyvitamin D2/D3 on Raptor FluoroPhenyl by LC-MS/MS

LC_CF0644

Column	Raptor FluoroPhenyl (cat.# 9319A1E)
Dimensions:	100 mm x 3 mm ID
Particle Size:	2.7 µm
Temp.:	30 °C
Sample
Diluent:	Water:methanol (50:50)
Conc.:	25-50 ng/mL
Inj. Vol.:	5 µL
Mobile Phase
A:	0.1% Formic acid in water
B:	Methanol
	Time (min) Flow (mL/min) %A %B 0.00 0.6 25 75 4.00 0.6 15 85 4.10 0.6 0 100 5.00 0.6 0 100 5.01 0.6 25 75 7.00 0.6 25 75
Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	HPLC

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