Warfarin and Chemotherapeutic Drugs on Raptor FluoroPhenyl by LC-MS/MS

LC_CF0678
Column | Raptor FluoroPhenyl (cat.# 9319A12) | ||||||||||||||||||||
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||
Guard Column: | Raptor FluoroPhenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9319A0252) | ||||||||||||||||||||
Temp.: | 50 °C | ||||||||||||||||||||
Sample | |||||||||||||||||||||
Diluent: | Water:methanol (95:5) | ||||||||||||||||||||
Conc.: | 10-100 ng/mL | ||||||||||||||||||||
Inj. Vol.: | 10 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||
B: | Methanol | ||||||||||||||||||||
| |||||||||||||||||||||
Max Pressure: | 400 bar | ||||||||||||||||||||
Detector | MS/MS | ||||||||||||||||||||
Ion Mode: | ESI+ | ||||||||||||||||||||
Mode: | MRM | ||||||||||||||||||||
Instrument | UHPLC | ||||||||||||||||||||
Notes | The sample solution was prepared by protein precipitation using Resprep PPT3 96-well plates (cat# 26489) and reservoir (cat# 26493). A 200 μL aliquot of fortified human plasma was mixed with 600 μL of acetonitrile by vortexing for 30 seconds at 2,000 rpm. The sample was filtered using a vacuum manifold. The filtrate was dried and reconstituted with 100 μL of water:methanol (95:5) and injected for analysis. |
RELATED CHROMATOGRAM LIBRARY SEARCHES
warfarin
,Raptor FluoroPhenyl
,FluoroPhenyl
,5-fluorouracil
,methotrexate
,carboplatin
,ifosfamide
,cyclophosphamide
,etoposide
Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.