Warfarin and Chemotherapeutic Drugs on Raptor FluoroPhenyl by LC-MS/MS

	Peaks	tR (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	Carboplatin	0.83	100	372.00	294.00	355.00
2.	5-Fluorouracil	0.89	100	130.98	113.99	57.99
3.	Methotrexate	2.18	20	455.15	308.09	175.14
4.	Ifosfamide	2.67	20	261.03	91.95	153.90
5.	Cyclophosphamide	2.75	20	261.03	139.97	106.01
6.	Etoposide	3.10	100	589.34	229.01	185.03
7.	Warfarin	3.58	10	309.11	162.99	251.01

LC_CF0678

Column	Raptor FluoroPhenyl (cat.# 9319A12)
Dimensions:	100 mm x 2.1 mm ID
Particle Size:	2.7 µm
Pore Size:	90 Å
Guard Column:	Raptor FluoroPhenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9319A0252)
Temp.:	50 °C
Sample
Diluent:	Water:methanol (95:5)
Conc.:	10-100 ng/mL
Inj. Vol.:	10 µL
Mobile Phase
A:	0.1% Formic acid in water
B:	Methanol
	Time (min) Flow (mL/min) %A %B 0.00 0.4 95 5 4.00 0.4 0 100 4.01 0.4 95 5 6.00 0.4 95 5
Max Pressure:	400 bar
Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Notes	The sample solution was prepared by protein precipitation using Resprep PPT3 96-well plates (cat# 26489) and reservoir (cat# 26493). A 200 μL aliquot of fortified human plasma was mixed with 600 μL of acetonitrile by vortexing for 30 seconds at 2,000 rpm. The sample was filtered using a vacuum manifold. The filtrate was dried and reconstituted with 100 μL of water:methanol (95:5) and injected for analysis.

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RELATED CHROMATOGRAM LIBRARY SEARCHES

warfarin

,

Raptor FluoroPhenyl

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FluoroPhenyl

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5-fluorouracil

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methotrexate

,

carboplatin

,

ifosfamide

,

cyclophosphamide

,

etoposide

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