The Analysis of Morphine & Metabolites in Urine without Hydrolysis on Force Biphenyl

	Peaks	tR (min)	Precursor Ion	Product Ion	Product Ion
1.	Morphine-3-β-D-glucuronide	0.61	462.1	286.0	152.1
2.	Morphine	1.06	286.1	152.0	164.9
3.	Morphine-6-β-D-glucuronide	1.17	462.1	286.0	152.1
4.	Morphine-N-oxide	1.30	302.1	284.9	161.9
5.	6-Monoacetylmorphine	1.81	328.1	211.1	165.0
6.	Hydrocodone	1.91	300.3	199.2	128.3

LC_CF0680

Column	Force Biphenyl (cat.# 9629252)
Dimensions:	50 mm x 2.1 mm ID
Particle Size:	1.8 µm
Guard Column:	UltraShield UHPLC precolumn filter, 0.2 µm frit (cat.# 25809)
Temp.:	40 °C
Sample
Conc.:	A 750 ng/mL standard was prepared in 1 mL of urine. 100 μL of the fortified urine standard was diluted with 900 μL of 0.1% formic acid in water prior to analysis.
Inj. Vol.:	2 µL
Mobile Phase
A:	0.1% Formic acid in water
B:	0.1% Formic acid in methanol
	Time (min) Flow (mL/min) %A %B 0.00 0.5 85 15 0.50 0.5 85 15 2.00 0.5 30 70 2.01 0.5 85 15 4.00 0.5 85 15
Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC

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