Steroid Panel Analysis on Force Biphenyl

	Peaks	tR (min)	Q1	Q3 Quantifier	Q3 Qualifier
1.	Cortisol	2.3	363.3	121.2	327.2
2.	Estradiol	2.6	255.3	159.1	133.1
3.	11-Deoxycortisol	4.0	347.4	109.2	97.1
4.	DHEA	4.3	271.3	253.2	213.2
5.	Boldenone	4.4	287.2	121.2	135.2
6.	Corticosterone	4.4	347.4	329.2	121.1
7.	Stanozolol	4.4	329.3	81.1	95.1
8.	Epitestosterone	4.5	289.2	109.2	97.1
9.	17-OH-progesterone	4.8	331.3	97.2	109.5
10.	Testosterone	5.0	289.2	109.1	97.0
11.	Trenbolone	5.1	303.3	109.2	97.1
12.	Methyltestosterone	5.1	271.2	253.1	199.2
13.	Nandrolone	5.3	275.2	109.2	257.3
14.	25-Hydroxyvitamin D3	6.1	383.3	365.3	107.1
15.	Androstenedione	6.2	287.2	97.1	109.2
16.	Progesterone	7.5	315.3	109.2	97.1

LC_CF0702

Column	Force Biphenyl LC Column (cat.# 9629512)
Dimensions:	100 mm x 2.1 mm ID
Particle Size:	5 µm
Pore Size:	100 Å
Temp.:	30 °C
Sample	Steroids panel
Diluent:	50:50 Methanol:water
Conc.:	5 ng/mL
Inj. Vol.:	50 µL
Mobile Phase
A:	Water, 0.1% formic acid
B:	Methanol, 0.1% formic acid
	Time (min) Flow (mL/min) %A %B 0.00 0.4 30 70 8.00 0.4 0 100 8.01 0.4 30 70 10.00 0.4 30 70
Max Pressure:	250 bar
Detector	MS/MS
Ion Mode:	ESI+
Mode:	Scheduled MRM
Instrument	UHPLC
Notes

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