Catecholamines and Metabolites in Human Urine on Raptor Biphenyl by LC-MS/MS
|Column||Raptor Biphenyl (cat.# 9309A62)|
|Dimensions:||150 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Inj. Vol.:||2 µL|
|A:||Water, 0.2% formic acid|
|B:||Methanol, 0.2% formic acid|
|Notes||Solid Phase Extraction Procedure:|
A 200 µL aliquot of urine sample (Bio-Rad Lyphochek quantitative urine control, normal level) was mixed with 10 µL (1 µg/mL in methanol) internal standard solution and 600 μL of 250 mM ammonium acetate solution. The mixture was loaded onto the EVOLUTE EXPRESS WCX 96-well plate (30 mg), washed with 1 mL water and 1 mL methanol:acetonitrile (60:40), eluted with 200 µL of 5% formic acid in water:methanol (95:5), and injected (2 μL) for analysis.
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