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EtG/EtS in UTAK EtG Plus Level 1 Urine Control on Raptor EtG/EtS by LC-MS/MS

PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Ethyl-β-D-glucuronide0.81500221.2075.185.1
2.Ethyl-β-D-glucuronide-d50.81100226.2085.00-
3.Ethyl sulfate-d51.84100130.1098.05-
4.Ethyl sulfate1.87200125.1097.1080.00
image
LC_CF0726
ColumnRaptor EtG/EtS (cat.# 9325A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809)
Temp.:35 °C
Sample
Ethyl-β-D-glucuronide-d5 (EtG-d5) (cat.# 34102)
Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104)
Diluent:0.1% Formic acid in water
Conc.: UTAK EtG Plus urine quality control (200 ng/mL EtS and 500 ng/mL EtG)
Inj. Vol.:10 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.5955
2.500.56535
2.510.5955
4.000.5955
DetectorMS/MS
Ion Source:Electrospray
Ion Mode:ESI-
Mode:MRM
InstrumentHPLC
NotesUTAK EtG Plus Level 1 control contains EtG at 500 ng/mL and EtS at 200 ng/mL concentrations. A 50 μL aliqout was taken from the the Level 1 control and diluted 20 times with 950 μL of a working internal standard (100 ng/mL EtG-d5 and EtS-d5 in 0.1% formic acid in water). The sample was vortexed at 3000 rpm for 10 seconds to mix. The sample was then centrifuged at 3000 rpm for 10 minutes at 10 °C and 10 μL was injected for LC-MS/MS analysis.
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