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Phosphatidylethanol 16:0/18:1 in Human Whole Blood on Raptor FluoroPhenyl

PeakstR (min)Conc.Precursor IonProduct Ion
1.Phosphatidylethanol-d5 (PEth-d5) 16:0/18:11.580.4 μM706.6281.3
2.Phosphatidylethanol (PEth) 16:0/18:11.580.5 μM701.5255.3
image
LC_CF0733
ColumnRaptor FluoroPhenyl (cat.# 9319A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Conc.:0.5 μM in blood
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium acetate
B:9:1 Methanol:2-propanol
Time (min)Flow (mL/min)%A%B
0.000.53070
1.000.52080
2.000.52080
2.500.50100
2.510.53070
3.500.53070
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentUHPLC
NotesSample Preparation Procedure (Provided by Redhot Diagnostics):
The fortified blood sample (50 µL) was mixed with 50 µL of internal standard (0.4 µM PEth-d5 in 2-propanol) and 150 µL of 4:1 2-propanol:tetrahydrofuran. The mixture was vortexed for 20 seconds at 3000 rpm and centrifuged for 10 minutes at 4300 rpm. The supernatant (2 µL) was injected for analysis.
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