Perfluorinated Alkyl Acids on Raptor C18 by LC-MS/MS for EPA Method 537

	Peaks	tR (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	Perfluorobutanesulfonic acid (PFBS)	1.98	10	299.10	79.99
2.	Perfluoro-n-[1,2-13C2]hexanoic acid (13C-PFHxA)	2.92	10	315.13	270.13
3.	Perfluorohexanoic acid (PFHxA)	2.93	10	313.10	269.12
4.	Perfluoroheptanoic acid (PFHpA)	4.00	5	363.16	319.09
5.	Perfluorohexanesulfonic acid (PFHxS)	4.14	10	399.13	79.98
6.	Perfluoro-[1,2-13C2]octanoic acid (13C-PFOA)	4.83	5	415.13	370.10
7.	Perfluorooctanoic acid (PFOA)	4.83	5	413.16	369.10
8.	Perfluorononanoic acid (PFNA)	5.50	5	463.16	419.19
9.	Perfluoro-1-[1,2,3,4-13C4]octanesulfonic acid (13C-PFOS)	5.54	5	503.13	79.98
10.	Perfluorooctanesulfonic acid (PFOS)	5.54	10	499.17	79.98
11.	Perfluoro-n-[1,2-13C2]decanoic acid (13C-PFDA)	6.06	5	515.17	470.17
12.	Perfluorodecanoic acid (PFDA)	6.07	5	513.17	469.16
13.	N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-NMeFOSAA)	6.28	5	573.23	419.15
14.	N-methyl perfluorooctanesulfonamidoacetic acid (NMeFOSAA)	6.28	5	570.20	419.17
15.	N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-NEtFOSAA)	6.52	5	589.27	419.11
16.	N-ethyl perfluorooctanesulfonamidoacetic acid (NEtFOSAA)	6.52	5	584.20	419.18
17.	Perfluoroundecanoic acid (PFUnA)	6.56	5	563.23	519.24
18.	Perfluorododecanoic acid (PFDoA)	6.98	5	613.23	569.19
19.	Perfluorotridecanoic acid (PFTrDA)	7.35	5	663.23	619.21
20.	Perfluorotetradecanoic acid (PFTA)	7.67	5	713.23	669.23

LC_EV0548

Column	Raptor C18 (cat.# 9304552)
Dimensions:	50 mm x 2.1 mm ID
Particle Size:	5 µm
Pore Size:	90 Å
Temp.:	40 °C
Sample
Diluent:	96:4 Methanol:water
Conc.:	5–10 ng/mL
Inj. Vol.:	2 µL
Mobile Phase
A:	5 mM ammonium acetate in water
B:	Methanol
	Time (min) Flow (mL/min) %A %B 0.00 0.4 75 25 8.00 0.4 15 85 8.01 0.4 75 25 9.00 0.4 75 25
Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Mode:	MRM
Instrument	Waters ACQUITY

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Raptor C18

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EPA 537

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perfluorinated chemical

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fluorochemical

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Raptor

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PFOA

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PFOS

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perfluorinated

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perfluorinated alkyl acid

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PFAS

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