Mycotoxins in Yellow Corn Meal on Raptor Biphenyl by LC-MS/MS

	Peaks	tR (min)	Conc. (ng/g)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Deoxynivalenol	0.62	50	297.3	249.3	231.2
2.	Fumonisin B1	2.45	50	722.5	352.4	334.5
3.	HT-2	2.60	50	447.3	285.3	345.3
4.	Fumonisin B3	2.85	50	706.5	336.4	318.4
5.	Fumonisin B2	3.23	50	706.5	336.3	141.2
6.	T2	3.31	50	489.3	245.2	387.4
7.	Aflatoxin G2	3.74	5	331.2	313.3	189.3
8.	Zearalenone	3.96	50	319.3	283.3	187.2
9.	Aflatoxin G1	4.22	5	329.2	243.2	200.2
10.	Aflatoxin B2	4.43	5	315.3	287.3	259.2
11.	Aflatoxin B1	4.99	5	313.3	285.2	241.2
12.	Ochratoxin A	5.19	5	404.2	239.3	358.3

LC_FS0525

Column	Raptor Biphenyl (cat.# 9309A52)
Dimensions:	50 mm x 2.1 mm ID
Particle Size:	2.7 µm
Pore Size:	90 Å
Guard Column:	Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:	40 °C
Inj. Vol.:	5 µL
Mobile Phase
A:	Water, 2 mM ammonium formate, 0.1% formic acid
B:	Methanol, 2 mM ammonium formate, 0.1% formic acid
	Time (min) Flow (mL/min) %A %B %C 0.00 0.5 70 30 0.6 0.5 70 30 0.7 0.5 50 50 3.0 0.5 30 70 4.5 0.5 25 75 5.0 0.5 10 90 5.2 0.5 10 90 5.21 0.5 25 75 6.00 0.5 25 75 6.01 0.5 70 30 7.00 0.5 70 30
Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Notes	Weighed 1.00 gram of corn meal in a 50-mL centrifuge tube and added 2.00 mL of water. Vortexed at 3000 rpm for 5 min followed by the addition of 4.0 mL of extraction solvent (50:50 water:acetonitrile, v/v). The tube was then vortexed at 3000 rpm for 5 min followed by centrifugation for 15 min at 4200 rpm. 475 μL of the supernatant was filtered through a Thomson SINGLE StEP Nano filter vial (0.2 μm, cat.# 25882). The sample was then fortified with 25 μL of a standard solution prepared in water at 1000 ng/mL (100 ng/mL for aflatoxins and orchratoxin A) as part of the matrix-matched calibration curve. Vortexed at 3000 rpm for 1 min prior to analysis.

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