50 μL of a commerically available CBD product was diluted in 950 μL of 25:75 water:methanol. The sample was vortexed for 30 seconds at 3000 rpm. 400 μL of the sample was then filtered using a Thomson SINGLE StEP standard filter vial (PVDF, 0.2 μm, Restek cat.# 25895) prior to analysis. CBDA, CBG, CBD, Δ9-THC, and CBC were detected, but only CBD was quantified.
Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.