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Cannabis Flower Sample on Raptor ARC-18 1.8 μm by UHPLC-UV
Cannabigerolic acid (CBGA)
Tetrahydrocannabinolic acid A (THCA-A)
Raptor ARC-18 (cat.# 931421E)
100 mm x 3.0 mm ID
UltraShield UHPLC precolumn filter (cat.# 25809)
Water, 5 mM ammonium formate, 0.1% formic acid
Acetonitrile, 0.1% formic acid
UV/Vis @ 228 nm
200 mg of ground flower was weighed into a 50 mL centrifuge tube. 10 mL of IPA was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 3000 rpm. The supernatant was diluted 50-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.
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