This chromatogram may contain analytes that are synonyms of your search terms.

Cannabinoids in Gummy Edibles on Raptor ARC-18

PeakstR (min)mg/g*
1.Cannabidiolic acid (CBDA)2.1420.2
2.Cannabigerol (CBG)2.4050.2
3.Cannabidiol (CBD)2.5350.2
4.Cannabinol (CBN)3.7760.2
5.Δ9-Tetrahydrocannabinol (Δ9-THC)4.7530.2
6.Tetrahydrocannabinolic acid A (THCA-A)6.2790.2
*Extract from a gummy sample initially spiked at 0.2 mg/g.
ColumnRaptor ARC-18 (cat.# 9314A62)
Dimensions:150 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor ARC-18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)
Temp.:30 °C
SampleCannabinoids standard (cat.# 34014)
Cannabigerol (cat.# 34091)
d9-Tetrahydrocannabinol (cat.# 34067)
d9-Tetrahydrocannabinolic acid A (cat.# 34111)
Diluent:75:25 Acetonitrile:water
Conc.: Expected concentration of 4 ppm in final extract from gummy initially spiked at 0.2 mg/g.
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
DetectorUV/Vis @ 228 nm
NotesGummies were manually chopped into small pieces, and 1 g of sample was weighed in a 50 mL polypropylene tube. The sample was mixed with 5 mL of water and then vigorously vortexed until all gummy pieces were fully solubilized. The sample was fortified with cannabinoids at 0.2 mg/g. The spiked sample was further vortexed for 30 sec. 5 mL of acetonitrile acidified with 1% acetic acid was added to the sample, and this was followed by 30 sec vortex agitation. Then, a pouch of European EN 15662 QuEChERS extraction salts (cat.# 25849) was added to the sample. The sample was vortexed for 30 sec and then centrifuged for 5 min. 100 µL of organic extract was mixed with 900 µL of 75:25 acetonitrile:water. 2 µL of final extract was injected into the HPLC-UV system.
Download PDF









Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.

Restek Domestic Customer Service



Your Full Name

Your Email

Company Name


Spam Block (Please leave this blank)

all fields required

Thank you

Your message has been sent. We will be in touch shortly.

Message not sent

Sorry, your message could not be sent at this time. Please try again later, or contact Restek or your local Restek representative via phone.