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Ultra Aqueous C18 Columns (USP L1)

..., these columns are designed for selective and reliable HPLC applications.The Restek Aqueous C18 -purpose Aqueous C18 boasts high reproducibility and compatibility with many mobile phase conditions—even 100 ... ultra_co_hplc, structure_lc_aqueous-c18.png ... Ultra Aqueous C18 Columns ... AQUA C18, Aquasil C18, Hypersil Gold AQ, YMC ODS-Aq ... Stationary Phase Category: modified C18 (L1)Ligand Type: proprietary polar modified and functionally bonded C18Particle: 3 µm or 5 µm, sphericalPore Size: 100 ÅCarbon Load: 15%End...

Ultra Aqueous C18 HPLC Prep Columns

....The Restek Aqueous C18 is a rugged, reversed-phase column with a well-balanced retention profile. It can -MS analyses. The general-purpose Aqueous C18 boasts high reproducibility and compatibility with many mobile ... lc_prep_columns, structure_lc_aqueous-c18.png ... Ultra Aqueous C18 HPLC Prep Columns ... Aquasil C18, Atlantis dC18, Hydrosphere C18, Hypersil Gold AQ, Luna Omega Polar C18, Luna Omega PS C18, Zorbax SB-AQ...

Pinnacle DB Aqueous C18 Columns (USP L1)

.... Combine selectivity and efficiency by using Restek Pinnacle DB UHPLC columns.The Restek Aqueous C18 -purpose Aqueous C18 boasts high reproducibility and compatibility with many mobile phase conditions—even 100 ... pinnacledb_co_hplc, structure_lc_aqueous-c18.png ... Pinnacle DB Aqueous C18 Columns ... AQUA C18, Aquasil C18, Hypersil Gold AQ, YMC ODS-Aq ... Stationary Phase Category: modified C18 (L1)Ligand Type: proprietary polar modified and functionally bonded C18Particle: 1.9 µm, 3 µm, or 5 µm, sphericalPore Size: 140 ÅCarbon Load: 6...

Ultra Aqueous C18 Guard Cartridge

...Ultra Aqueous C18, 5um, 10x4.0mm Guard Cartridge, 3-pk....

Pinnacle DB Aqueous C18 Guard Cartridge

...Pinnacle DB Aqueous C18, 5um, 10x4.0mm Guard Cartridge, 3-pk....

HPLC Analysis of Glucosinolates in Vegetable Extracts without Ion Pairing Using an Ultra Aqueous C18 Column (PDF 675kB)

... Without Ion Pairing Using an Ultra Aqueous C18 Column Glucosinolates are a naturally occurring set standard using an Ultra Aqueous C18 column shows good peak shape without the use of ion-pairing reagents...

Tech Tip — Why do I Lose Retention on my C18 in Highly Aqueous Mobile Phases?

... compounds. The Aqueous C18 is a uniquely bonded C18 that resists de-wetting and is completely compatible development with a more versatile C18, like the Aqueous C18, can greatly speed up development and lessen ... Learn what de-wetting is, and how to avoid it, by using an Aqueous C18 column, instead of a conventional C18, when analyzing polar compounds by HPLC. ... de-wetting, Aqueous C18, polar compounds, reversed phase, RPLC...

Optimized RP-HPLC Method for Hydroxybenzoic Acids Balanced Retention for a Range of Polarities, Using an Ultra Aqueous C18 Column

... Useful retention of more polar and less polar analytes. Ultra Aqueous C18 phase[2]), and on an Ultra Aqueous C18 column. Our objective was to find the optimum stationary phase ... C18 phase assures high resolving power, the best separations across a broad range of analyte , creating differences in overall polarity and solubility. The unique bonding chemistry of the Ultra Aqueous ... Ultra, aqueous c18, hydroxybenzoic acoids, hydroxyl groups, analgesics...

Accurately Quantify 380 Pesticides Using LC-MS/MS and GC-MS/MS (PDF 6072kB)

... (cat.# 31972) on Ultra Aqueous C18 by LC-MS/MS. Figure 2: LC Multiresidue Pesticide Standard #2 (cat .# 31973) on Ultra Aqueous C18 by LC-MS/MS. LC_FF0517 LC_FF0518 Figure 1: LC Multiresidue Pesticide...

Restek LC Guard Column Selector: How to Find the Right Guard Fast (PDF 2037kB)

... Phases Ultra 3 C18, C8, C4, C1, Biphenyl, Aromax, Aqueous C18, PFP Propyl, Cyano, IBD, Amino, Silica , Quat, Carbamate C18, C8, C4, C1, Biphenyl, Aromax, Aqueous C18, PFP Propyl, Cyano, IBD, Amino, Silica...

Trident LC Column Protection System: Redesigned to be more rugged and easier to use! (PDF 1187kB)

... Cartridge 941450212 941450210 Pinnacle DB C8 Guard Cartridge 941350212 941350210 Pinnacle DB Aqueous C18 912750210 Ultra Aqueous C18 Guard Cartridge 917850212 917850210 Ultra Biphenyl Guard Cartridge 910950212...

Roc HPLC Columns: The Reliable Cornerstone for Your LC Lab (PDF 2233kB)

....5 to 8.0 2.5 to 8.0 Maximum Temperature 80 °C 80 °C 80 °C 80 °C 80 °C CH3 Si O C8 C18 X3 IBD Aqueous C Aqueous C18 Amino Silica Phenyl Phenyl Propyl Biphenyl PROPRIETARY Aromax CH3 Si CH3 O CH3 Si CH3 O...

ChromaBLOGraphy: Comprehensive Pesticide Screening Using QuEChERS, LC-MS/MS, and GCxGC-TOFMS at PittCon 2010

A recent collaboration with Applied Biosystems used a sensitive LC-MS/MS (4000 QTRAP) with a Restek Ultra Aqueous C18 column as the instrumental determination step for pesticides in QuEChERS extracts of red bell pepper, lemon, spinach, hazelnuts, raisins, and cucumber. Those same QuEChERS extracts were analyzed by a LECO Pegasus 4D GCxGC-TOFMS with Rxi-5Sil MS and [...]

ChromaBLOGraphy: Improve Retention and Peak Shape for Early-Eluting Pesticides in LC-MS/MS

How important is the HPLC column in a comprehensive LC-MS/MS screening method? LC-MS/MS is quickly gaining acceptance for residue testing, and can simultaneously monitor hundreds of pesticides, including those difficult to detect by GC. With such a powerful detector, hasn’t the column become simply a sample inlet device? While the MS/MS detector allows for specific, [...]

ChromaBLOGraphy: Can I use a 100% aqueous mobile phase with my LC column?

You might be asking this because you have read that not all columns are OK to use with highly aqueous content (>95%) in the mobile phase.  Or maybe the caption caught your eye because you have recently encountered some difficulty with using highly aqueous mobile phase. There is a phenomenon called “phase dewetting” that can […]

Restek LC Guard Column Selector: How to Find the Right Guard Fast

... Ultra 3 C18, C8, C4, C1, Biphenyl, Aromax, Aqueous C , C8, C4, C1, Biphenyl, Aromax, Aqueous C18, PFP Propyl, Cyano, IBD, Amino, Silica, Quat, Carbamate...

Multi-task with an Ultra IBD Column (PDF 130kB)

... to conventional and aqueous C18 phases. Because the Ultra IBD column possesses both nonpolar and highly polar 2008 vol. 1 • 18 • Pharmaceutical Reversed phase HPLC analyses are predominantly performed on C18...

ChromaBLOGraphy: A C18 is a C18, right?

    Sorry, that actually is not true. Although many methods only list the USP code L1 for all C18 HPLC columns, there are many different varieties of C18 columns available from Restek. Here are some of the ways they may differ:   Differences in Silica Depending on the silica particle that the C18 phase […]

ChromaBLOGraphy: Easy Peasy: Optimized LC-QTRAP Method for Pesticide Residues

Despite what is sometimes advertised, setting up optimized methods for multiresidue pesticide analysis is typically not “easy peasy” and can be quite time consuming. Luckily, some colleagues are willing to share their method with us. Thank you Kelli, James, and Jon!* The downloadable spreadsheet contains retention times, transitions, ionization and collision parameters for our LC […]

ChromaBLOGraphy: Which LC Phase Do I Need?

So you’re thinking about doing HPLC (or UHPLC) and don’t have a method? You’ve never even seen a method for anything remotely similar. You might have even searched our HPLC chromatogram database for the application and haven’t found anything.  (After all, that’s the quickest and easiest way to get some ideas.) What to do… Let’s [...]

Raptor Biphenyl LC Columns Sales Sheet (PDF 3731kB)

... groups Ultr a Aqueous C18HydrophobicDipolarAcidicBasic0246810Ultra IBDHydrophobic it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl...

Raptor Biphenyl LC Columns Brochure (PDF 6071kB)

...: Aqueous C18 IBD Biphenyl PFPP Dispersion H-Bonding Polarizability Cation Exchange Dispersion H .5–8.0 Maximum Temperature: 80 °C Maximum Pressure: 1,034 bar/15,000 psi* (1.8 μm), 600 bar/8,700 psi (2.7 μm...

ChromaBLOGraphy: Read This Before You Go to PittCon 2010 in Orlando!

The title is akin to “Free Beer” to get you to click in, but I invite my colleagues to visit me and Restek at PittCon. Due to space limitations, I only tout my presentations here (yeah right Jack, it’s because of space limitations…). I’m heavily invested in QuEChERS. Poster 660-2 P, Mon, comparing GPC to [...]

ChromaBLOGraphy: [4]Troubleshooting HPLC- Fronting Peaks

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we have looked at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. Any time you have difficulty with poor peak shape, it is also […]

ChromaBLOGraphy: More Technical Service “Red Flags” – LC

This post is the second of its kind pertaining to LC analysis, all of which are an extension of a series pertaining to “Red Flags” for GC analysis. These are situations and symptoms that tell us in the Tech Service group that something is just not right. As we discussed in the first post, Technical […]

ChromaBLOGraphy: Live Long and Prosper-How to make your HPLC columns last longer

We frequently get questions about how long an HPLC or UHPLC column should last. To borrow a phrase from my colleagues, “Your mileage may vary.”  Columns are like cars, so their lifetime depends how they are used and how well they are maintained. That being said, some phases are known to be more rugged than [...]

ChromaBLOGraphy: Choosing a guard cartridge for LC

So you have a guard cartridge holder of some sort, and you don’t have the guard cartridge to put inside. If you happen to have missed that last blog post about the holder, click on the following link. Choosing a guard cartridge holder for LC Let’s determine what type of guard cartridge you need. A […]

ChromaBLOGraphy: Do reverse phase HPLC columns really need conditioning?

  Well, it’s partly a matter of semantics sometimes, but there is usually nothing drastic that you need to do to get your HPLC column going if it is new.  There is no baking at high temperatures involved like you might do for GC or that sort of thing. You do, however, need to equilibrate […]

Restek LC Columns and Accessories (PDF 17409kB)

... • Fused-ring compounds with electron withdrawing groups Solute Retention Profile: Ultra Aqueous C18 • Dispersion Complementary Solute Interaction: • Cation exchange Column Interaction Profile: Aqueous C18...

Frequently Asked Questions: HPLC and UHPLC

... aqueous content, we suggest the Ultra Aqueous C18 or Pinnacle DB Aqueous C18 columns. Back to top of page...

For advanced chromatogram searching, please check out our Chromatogram Library Search Tool.

LC Multiresidue Pesticide Standard #4 on Ultra Aqueous C18 by LC-MS/MS

LC_FF0520

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Pesticides on Ultra Aqueous C18

LC_FF0490

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Pesticides on Ultra Aqueous C18

LC_FF0490A

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Pesticides on Ultra Aqueous C18

LC_FF0490B

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