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Chromatography Columns > HPLC & UHPLC Columns

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LC Performance Test Mix (5 components)

... that is highly effective for characterizing LC columns for efficiency, void volume, methylene selectivity , for column selection, for monitoring column performance over time, or for quality control. We test our ... Highly effective for characterizing LC column parameters. Simple, easy , reliable approach to quality control (QC) evaluations or column classification. Monitor column...

Rtx-BAC Plus 1 Columns (fused silica)

...These application-specific columns for blood alcohol analysis baseline separate all critical —in less than 2 minutes. Every Rtx-BAC Plus 1 and Rtx-BAC Plus 2 column is qualified with a test mix ... Rtx-BAC Plus 1 Columns ... Chromatography Columns , GC Columns , Fused Silica Capillary ... Don’t forget: Blood alcohol analysis requires dual column confirmation when using GC /FID. Click here for information on the secondary column for this application. ... Optimized column selectivities guarantee resolution of ethanol, internal standards , and frequently encountered interferences. Robust and reproducible column chemistry ensures longer...

Rtx-BAC Plus 2 Columns (fused silica)

...These application-specific columns for blood alcohol analysis baseline separate all critical -propanol—in less than 2 minutes. Every Rtx-BAC Plus 1 and Rtx-BAC Plus 2 column is qualified with a test mix ... Rtx-BAC Plus 2 Columns ... Chromatography Columns , GC Columns , Fused Silica Capillary ... Don’t forget: Blood alcohol analysis requires dual column confirmation when using GC /FID. Click here for information on the primary column for this application. ... Optimized column selectivities guarantee resolution of ethanol, internal standards , and frequently encountered interferences. Robust and reproducible column chemistry ensures longer...

Rxi-1301Sil MS Columns (fused silica)

midpolarity Crossbond phase

... stationary phase that not only provides the column selectivity needed for analyzing less volatile compounds Cyano-based 1301 columns are general use GC columns that are well suited for the analysis ... Rxi-1301Sil MS Columns ... Chromatography Columns , GC Columns , Fused Silica Capillary ... of glycol ethers.Click here for more information on our Rxi columns. ... and increased uptime. Stabilized cyano phase selectivity improves the performance of existing methods...

USLC Columns Put the Right Tools in Your LC Method Development Toolbox (PDF 1474kB)

... Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column , is column selectivity. Unfortunately, column selectivity is also the least understood and most ... column selectivity, peak separation, stationary phase, chromatographic resolution, USLC...

USLC Columns: Choose Columns Fast. Develop Methods Faster. (PDF 1815kB)

...RESTEK USLC Ultra Selective Liquid Chromatography Choose Columns Fast. Develop Methods Faster . Ultra Selective Liquid Chromatography Technology Choose Columns Fast. Develop Methods Faster. What ... column selectivity, peak separation, stationary phase, chromatographic resolution...

GC Column Selection Poster

... up humdrum lab space with this attractive, easy-to-understand GC column selection poster. Simple gradient charts for column stationary phase, film thickness, inner diameter, and length make it easy ... Zip up humdrum lab space with this attractive, easy-to-understand GC column selection poster . Simple gradient charts for column stationary phase selectivity, film thickness, internal diameter...

USLC Column Selection & Mobile Phase Adjustment Guide (PDF 1682kB)

...RESTEK USLC Ultra Selective Liquid Chromatography Choose Columns Fast. Develop Methods Faster . USLC Column Selection & Mobile Phase Adjustment Guide Ultra Selective Liquid Chromatography (USLC ... , method development, stationary phase, column selectivity...

Restek GCxGC Columns for Comprehensive Two-Dimensional Gas Chromatography

Restek is your one source for 2D gas chromatography! Also known as GCxGC, comprehensive two-dimensional gas chromatography is a powerful technique th...

Guide to GC Column Selection and Optimizing Separations Brochure (PDF 3879kB)

...Guide to GC Column Selection and Optimizing Separations • Learn how to choose the right column analysis for both separation and speed becomes easier. This GC column selection guide discusses the basics...

Increase Data Quality for Sulfonamide Antibiotics Analysis by HPLC and UHPLC (PDF 975kB)

... Using Unique Biphenyl Column Selectivity By Rick Lake and Ty Kahler • Improve reporting accuracy with better selectivity and retention. • Biphenyl column and MS-friendly mobile phases allow easy transfer...

Method Development and Column Selection: How the FluoroPhenyl Phase Provides the Power of HILIC and Reversed-Phase Modes in One Column (PDF 2146kB)

...Figure 1: Raptor FluoroPhenyl LC Column Properties. Abstract Column selection is an important by the retention and selectivity of the analytical column. Although C18 columns are commonly employed, Fluoro...

ChromaBLOGraphy: Column selection: Is there more to life than using a boiling point column?

Last week I presented an invited talk at the Chromatographic society in the UK. http://www.restek.com/news/view/?p=797 Alan Handley, driving force behind the GC meetings, gave me a working title: “Column selection: is there is more to life than using a boiling point column?   The answer is clearly “yes”, not because I like chromatography but also […]

ChromaBLOGraphy: Totally serious analysis of N2O – capillary column selection

A few years ago, Jaap wrote a blog, “Can I analyze NO, N2O and NO2 via GC?”, which received a vast response. If you are just starting to dive into the analysis of N2O, scroll through the blog comments where you can find many valuable insights from our blog readers. What I would like to […]

Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC Using Unique Biphenyl Column Selectivity

.... Increase Accuracy With Ultra Selective Biphenyl Columns Since selectivity is the most important , for this work. Due to the column’s unique selectivity and high retention, we were able to develop a very ... The unique selectivity and high retention of Biphenyl columns produce complete separation of sulfonamide residues and benefit both UV and MS detection. In addition, Biphenyl columns in a UHPLC format...

Fast, Definitive Data for Blood Alcohol Testing on Rtx-BAC Plus 1 and Rtx-BAC Plus 2 Columns (PDF 963kB)

... in selectivity and inertness, coelution and tailing peaks are observed on competitor columns, which make . • Optimized column selectivities guarantee resolution of ethanol, internal standards, and frequently...

GC Columns Poster Package

... an overview to GC column selection and the second provides helpful tips for GC troubleshooting. Concepts from Column Selection poster ... an overview to GC column selection and the second provides helpful tips for GC troubleshooting. Concepts from . Request your GC Columns poster package today!...

Method Development and Column Selection: How the FluoroPhenyl Phase Provides the Power of HILIC and Reversed-Phase Modes in One Column

...Abstract Column selection is an important part of method development as the quality of critical separations is determined in large part by the retention and selectivity of the analytical column ... Column selection is an important part of method development as the quality of critical separations is determined in large part by the retention and selectivity of the analytical column. Although C18 columns...

News: Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection

Author(s): Rick Lake, Ty Kahler Published By: Restek Corporation Year of Publication: 2011 Link: http://www.restek.com/Technical-Resources/Technical-Library/Pharmaceutical/general_PHAN1348 Abstract: Column selectivity has the most significant influence on chromatographic peak separation, or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method … Continue reading

News: Restek to Present “Gas Chromatography: Foundation Theory, Column Selection, and Troubleshooting” at GC-MS Seminar

Chromatographers from Restek will be presenting at Waters’s upcoming seminar on October 23, 2019, in Wood Dale, IL. This educational opportunity is aimed at scientists looking to learn more about GC-MS capabilities in all areas, particularly in food and environmental … Continue reading

Analysis of FAMEs in Biodiesel Fuel: Pro EZGC Modeling Software Ensures Proper Column Selection

... glycol columns (FAMEWAX) offer excellent retention and selectivity for polar FAME compounds output to empirical data. Experimental Rt-2330 and FAMEWAX columns were selected ... Polar columns were evaluated for the analysis of fatty acids methyl esters (FAMEs) in finished B100 cyano phase Rt-2330 column and a polyethylene glycol phase FAMEWAX column were compared. The modeling...

ChromaBLOGraphy: Using GC column selectivity to overcome potential coelutions when switching to nitrogen carrier gas

The chromatograms below are from the PittCon presentation I mentioned in my earlier post.  The first set of two shows a translated method from hydrogen to nitrogen carrier gas.  The flow rates, linear velocities, and GC oven program rates for hydrogen versus nitrogen are different (but optimum for each gas).  As you can see, the […]

News: Analysis of FAMEs in Biodiesel Fuel: Pro EZGC Modeling Software Ensures Proper Column Selection

Author: Katarina Oden Published By: Restek Corporation Year of Publication: 2018 Link: http://www.restek.com/Technical-Resources/Technical-Library/Petroleum-Petrochemical/petro_PCAN2889-UNV Abstract: Polar columns were evaluated for the analysis of fatty acids methyl esters (FAMEs) in finished B100 biodiesel according to method EN 14103 (2011). Using Restek’s Pro EZGC chromatogram modeler, a … Continue reading

Highly Selective LC-MS/MS Analysis of Mycotoxins from Multiple Classes (PDF 556kB)

...: Mycotoxins on Raptor FluoroPhenyl • Unique column selectivity allows simultaneous determinations of 20 the Raptor FluoroPhenyl column’s selectivity allows compounds that are similar in structure and that elute...

News: Method Development and Column Selection: How the FluoroPhenyl Phase Provides the Power of HILIC and Reversed-Phase Modes in One Column

Author: Sharon Lupo, Frances Carroll Restek Corporation Published By: Restek Corporation Year of Publication: 2017 Link: http://www.restek.com/Technical-Resources/Technical-Library/General-Interest/gen_GNAR2613 Abstract: Column selection is an important part of method development, as the quality of critical separations is determined in large part by the retention and selectivity of … Continue reading

The Role of Selectivity in Liquid Chromatography Method Development

... is determined by three main factors: efficiency, selectivity, and retention. The interplay #945; is selectivity, and k’2 is the capacity factor (or retention factor) for the later eluting peak ... of column selectivity in liquid chromatography method development....

USLC Columns Put the Right Tools in Your LC Method Development Toolbox Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection

... selectivity. Unfortunately, column selectivity is also the least understood and most underutilized parameter . In this article, we discuss column selection for reversed phase separations and, using the hydrophobic ... Column selectivity has the most significant influence on chromatographic peak separation , or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method development...

Organic Volatile Impurities: Retention Time Index

...To make column selection for residual solvents easy, Restek has benchmarked the ICH Class 1, 2 and 3 residual solvents on our most popular OVI columns....

Restek Advantage, 2011.1 (PDF 3826kB)

...Restek Advantage, 2011.1 Introducing…Rxi-17SilMS Columns Offering Unique Selectivity for PAHs other “17”type columns.•Optimized selectivity separates a wide range of key PAHs.•Rxi technology assures...

LC-MS/MS Analysis of Urinary Free Cortisol and Cortisone without Matrix Interferences (PDF 515kB)

... and cortisone and the potential for matrix effects from urine components, appropriate column selectivity selective Raptor Biphenyl column. Chromatographic separation was essential in this case because some...

Restek Advantage, 2012.1 (PDF 14846kB)

...…pp. 14–15 Column/mobile phase selection for LC-MS…pp. 18–19 Trace impurities in petroleum gases…pp. 20 for Sulfonamides by HPLC and UHPLC Using Unique Biphenyl Column Selectivity...

Helium, Hydrogen, or Nitrogen—The Choice is Yours: Unique Rtx-CLPesticides Column Set Provides Optimal Results for Organochlorine Pesticides GC-Micro-ECD Analysis Using Any Carrier Gas (PDF 930kB)

... an electron capture detector (ECD) are generally good candidates for transfer as long as column selectivity GC-ECD method. These column sets differ in selectivity, and since proprietary Rtx...

New PLOT Column Technology Stabilises Flow and Improves Analysis of Light Hydrocarbons, Solvents and Permanent Gases (PDF 1947kB)

... and leads to more accurate quantification. Similar benefits are seen for other PLOT column selectivities degree of resolution. Selectivity can be changed using different deactivation salts; columns deactivated...

Restek GCxGC Columns: Your One Source for 2D Gas Chromatography (PDF 471kB)

...-XLB, and Stabilwax column. • Comprehensive kit simplifies column selection for method developers and frequent GCx the effectiveness of your GCxGC system. Application Area Primary Column Secondary Column Phase Selectivity...

Rtx-BAC Plus 1 and Rtx-BAC Plus 2 Columns (PDF 5471kB)

... Measurement of Alcohol in Blood • Optimized column selectivities guarantee resolution of ethanol, internal Rtx-BAC Plus 1 and Rtx-BAC Plus 2 columns have optimized column selectivities, which result...

NEW! Rx-1301Sil MS GC Columns (PDF 1237kB)

...NEW! Rxi-1301Sil MS GC Columns The Selectivity You Need Without the Bleed Figure 1: The new Rxi Restek is a silarylene-based cyano stationary phase that not only offers the column selectivity needed...

Restek Liquid Chromatography (LC) Columns - Usage & Care (PDF 143kB)

... can alter a column’s selectivity and, by plugging the inlet frit, significantly increase system Restek Liquid Chromatography (LC) Columns — Usage & Care Column Certificate of Analysis Each...

Optimized Volatiles Analysis Ensures Fast VOC Separations (PDF 1692kB)

... (trichlorobenzenes). These differences make column selectivity, thermal stability, and inertness critical to resolving volatiles. Often, “624” type columns are chosen for their selectivity, but thermal stability...

Ensure Successful Polycyclic Aromatic Hydrocarbon (PAH) Analysis—Choose Specialized Rxi-PAH Columns (PDF 7240kB)

... for these applications. Column dimensions were chosen to maximize efficiency and the selectivity of the proprietary . This combination of efficiency, selectivity, and robustness makes the Rxi-PAH column the best choice...

Raptor FluoroPhenyl LC Columns Brochure (PDF 3042kB)

...Stationary Phase: FluoroPhenyl Get the Power of HILIC and RP Modes in One LC Column Selectivity to a Raptor FluoroPhenyl LC column when: • You observe limited retention and selectivity on a C18 for basic...

How Column Inertness Improves the Chromatography of Basic Compounds (PDF 960kB)

... Make Column Selection EasyOften,when choosing an analytical capillary column,we focus entirely Pharmaceutical Applications How Column Inertness Improves the Chromatography of Basic Compounds...

Optimize Analysis of Polar and Coeluting VOCs in Whole Air Canister Samples Using an Rtx-VMS GC Column (PDF 335kB)

... flexibility in GC column selection both in terms of stationary phase and column dimensions. In regards to chromatographic column selection, section 7.2.2.2 of Method TO-15 states: “100% methyl silicone or 5% phenyl, 95...

Analysis of FAMEs in Biodiesel Fuel: Pro EZGC Modeling Software Ensures Proper Column Selection (PDF 921kB)

... and then compares the model output to empirical data. Experimental Rt-2330 and FAMEWAX columns were selected analysis. Both columns were initially evaluated using two criteria: selectivity using Pro EZGC chromatogram...

News: Restek to Present at “Innovations in GC-MS Technologies for Food and Agricultural Product Analysis” Seminar

Chromatographers from Restek will be presenting at Waters’s upcoming seminars on April 2 in Greenbelt, MD, and April 4, in Morrisville, NC. These educational opportunities are aimed at scientists analyzing food and agricultural products using GC-MS. Topics include chromatographic theory, … Continue reading

Rxi GC Columns Sales Sheet (PDF 3173kB)

... samples. GCxGC involves two columns with differing stationary phase selectivity (orthogonal column that is orthogonal (has different selectivity) to the primary column, it is possible to separate...

Analyze Seven EPA Methods on One GC Column Pair! Pesticides, PCBs, Herbicides, and More on Rtx-CLPesticides & Rtx-CLPesticides2 Columns (PDF 5571kB)

... a unique selectivity and are ideal for multiple GC-ECD methods. Compare them to your current column set selectivities. • Reduced contamination Guard column traps nonvolatile residue, protecting the analytical...

Featured Application: Mycotoxins on Raptor FluoroPhenyl Highly Selective LC-MS/MS Analysis of Mycotoxins from Multiple Classes

... column selectivity allows simultaneous determinations of 20 mycotoxins. Complete separation FluoroPhenyl column’s selectivity allows compounds that are similar in structure and that elute closely ... on the global economy. This highly selective LC-MS/MS analysis of 20 mycotoxins in just 9 minutes allows...

ChromaBLOGraphy: Which LC column should I use for my method?

We understand that with so many products on the market, choosing a column to get started can be difficult. Of course, column selection depends on what type of method you are following and what kind of LC system you have in your lab. Let’s begin by looking at our choices for the more traditional, fully […]

ChromaBLOGraphy: Analysis of Highly Toxic gases : Column Choice

A little follow up on the interesting topic that was blogged by Alan.  I promised to add a few comments on column selection as I have had several questions of this nature.   In general in gas analysis, you need to use columns with high retention. So only thick-film liquid phases like Rtx-1 or adsorption […]

ChromaBLOGraphy: How to choose the correct GC column – Part 1

A month or so ago I gave a short training session to a few beginners on how to go about choosing a GC column. After all, there are thousands of Restek column catalog numbers to choose from (not to mention numerous custom catalog numbers), so if an analyst doesn’t have a method which specifies which [...]

ChromaBLOGraphy: Maybe a rose is a rose is a rose, but a “5” is not a “5”, when it comes to pesticide analysis…

Greetings from South Africa, where I did a recent lecture on GC column selectivity and got the age-old question, “Are all “5” phases (5% diphenyl type) the same?” One easy answer, and you’ll certainly notice it if you are a pesticide chemist, is that the Rxi-5ms, a pendant-based 5% phenyl column, is definitely NOT the [...]

ChromaBLOGraphy: How to choose the correct GC column – Part 2

My last post looked at how to use published boiling points of compounds to help select a GC column. In this post, I will be discussing the role of compound polarity when selecting a GC column.   To briefly summarize my column selection process (discussed in Part 1 http://blog.restek.com/?p=5426 )   My four categories of [...]

Guide to Preparing and Analyzing Semivolatile Organic Compounds (PDF 6275kB)

... Chromatography pg. 5 Analysis Calibration standards pg. 6 Injection port configurationpg. 6 Column selection pg of the column when using this technique (see the Column Selection section for more information). 9 Any...

News: Restek Advantage

Published By: Restek Corporation Issue: 2013.1 Year of Publication: 2013 Link: http://www.restek.com/pdfs/GNAD1795-UNV.pdf Abstract: The Restek Advantage is a technical magazine covering industry news, chromatography applications, and guest editorials by leaders from a wide range of fields. Highlights from this issue … Continue reading

Restek Essentials: Featured Chromatography Products 2019.2 (PDF 5798kB)

... wide selection of instrument replacement parts and supplies. Don’t stop at the column; outfit your .5-5 microliter [Remove] Clear All Selections Running ASTM Methods? Find Your GC Columns, Reference Standards...

Restek Essentials: Featured Chromatography Products 2019.3 (PDF 6194kB)

... reliably be achieved with a GC Accelerator kit. Select a Scaled-Down Rxi Column Select an Rxi column ) Selectivity Kit kit 15131 For guidance on which column to use when, visit www...

Restek GCxGC Columns: Your One Source for 2D Gas Chromatography (PDF 9170kB)

... columns with differing stationary phase selectivity (orthogonal) that are press-fitted together that is orthogonal (has different selectivity) to the primary column, it is possible to separate and identify...

Featured Application: Urinary Free Cortisol and Cortisone on Raptor Biphenyl LC-MS/MS Analysis of Urinary Free Cortisol and Cortisone without Matrix Interferences

... components, appropriate column selectivity is critical for obtaining accurate results. In the LC from endogenous matrix interferences using a highly selective Raptor Biphenyl column. Chromatographic ... than immunoassay-based methods. However, it is critical that the analytical column has the proper selectivity for chromatographically separating cortisol from isobaric matrix components. In the method...

A Guide to the Analysis of Halogenated Environmental Pollutants Using Electron Capture Detection (PDF 8131kB)

... Analyzing Halogenated Pollutants..... 7 • Column Selection..............................................7 Selection A wide variety of columns have been used for analyzing chlorinated pesticides and herbicides...

PFAS LC Column Anatomy: Which Phase, Dimensions, and Particle Type Are Best? (PDF 1086kB)

... the development of better methods, and LC column selection is the foundation for building an improved approach analysis. Column Phase Selection The first decision to make when determining which PFAS LC column to use...

The Restek Biphenyl: Leading Resolution in LC since 2005

With ten years of unrivaled performance, Restek's time-tested Biphenyl has been proven to be the ideal LC column for clinical diagnostic, pain, pharm...

Novel Column Chemistry—High Impact, Low Cost Technology

... more beneficial. A Biphenyl column shows markedly greater aromatic selectivity and hydrophobic for selective column phases, is just as great as in HPLC. While UHPLC does produce significant gains ... Novel column chemistries are a simple change in an already budgeted consumable that can lead...

Method 552.2: Determination of Haloacetic Acids and Dalapon Analysis in Drinking Water by Ion Exchange Liquid-Solid Extraction and GC with an Electron Capture Detector

... for column selection is the degree of resolution between the methylated haloacetic acid compounds and known .2 and an appropriate GC column set, such as the Rtx-CLPesticides and the Rtx...

Optimized Volatiles Analysis Ensures Fast VOC Separations

... aromatic compounds (trichlorobenzenes). These differences make column selectivity, thermal stability , and inertness critical to resolving volatiles. Often, “624” type columns are chosen for their selectivity ... good resolution of critical pairs, while maximizing sample throughput. Rxi-624Sil MS columns are shown...

ChromaBLOGraphy: Cross reference lists for GC columns and other useful links

With everyone in such a hurry these days, when you cannot find what you need in a few minutes, how often do you give up and move onto your next project?   I’m the same way, so when I find a way to group commonly referenced information into one convenient area, I usually do it.  Below […]

MXT Metal GC Columns

Siltek-treated stainless steel MXT wall coated open tubular (WCOT) columns are the best choice for high-temperature chromatography and other situatio...

News: New GC Columns and Standards for Blood Alcohol Testing

Restek announces the release of new Rtx®-BAC Plus 1 and Rtx®-BAC Plus 2 columns for blood alcohol testing. These GC columns baseline separate all critical compounds, including ethanol, methanol, acetone, tert-butanol (IS), acetaldehyde, isopropanol, and 1-propanol (IS), in less than … Continue reading

ChromaBLOGraphy: Which LC column should I use for Method 8330B explosives analysis?

Over the years, Restek has run applications on a long list of columns for this analysis. It does require a primary and secondary column for analysis, since there is not one column that perfectly separates all compounds on the list simultaneously. Keep in mind that EPA Method 8330B allows for alternate columns versus the ones […]

Helium, Hydrogen, or Nitrogen—The Choice is Yours: Unique Rtx-CLPesticides Column Set Provides Optimal Results for Organochlorine Pesticides GC-Micro-ECD Analysis Using Any Carrier Gas

... good candidates for transfer as long as column selectivity is adequate. For heavily used methods in selectivity, and since proprietary Rtx-CLPesticides and Rtx-CLPesticides2 columns were developed specifically ... gases as a way to reduce helium consumption. These columns have a unique selectivity and produce faster Using an Rtx-CLPesticides column set is the best strategy for labs considering alternate carrier...

News: International GC Specialist Jaap de Zeeuw to Speak at Advances in GC IV Meeting

UPDATE: For a detailed review of Advances in GC IV, including more information on Jaap’s presentation, visit Chromatography Today’s Meeting Report. On October 30, 2013, The Chromatographic Society (ChromSoc) and the North West Region of the Analytical Division of the … Continue reading

Optimize Analysis of Polar and Coeluting VOCs in Whole Air Canister Samples Using an Rtx-VMS GC Column

... for a performance-based method. Therefore, there is considerable flexibility in GC column selection both in terms of stationary phase and column dimensions. In regards to chromatographic column selection, section 7 ... , labs have the option to use any GC column that meets performance criteria. Shorter columns can be used to reduce analysis times and stationary phases that separate difficult coelutions can be selected. After...

ChromaBLOGraphy: Terpenes in Medical Cannabis

Thanks to a colleague at SRI Instruments who sent me some terpenes, and with the use of other terpenes I had in house, I was able to collect numerous chromatograms of these compounds that may contribute to the “entourage effect“ of medical cannabis.  This means they may have therapeutic effects in their own right, or as synergists with cannabinoids. […]

ChromaBLOGraphy: RIP Philae

The exact resting place of the Philae lander on Comet 67P/Churyumov-Gerasimenko has finally been pinpointed, in new photographs by the Rosetta orbiter. Rosetta did a close pass of the comet on September 2nd and the survey caught the first clear glimpse of Philae’s position.   I’m so proud that Restek was able to contribute columns […]

News: Join Restek at the Forensic Science Symposium Offered by Shimadzu

Wednesday, August 22, 2018, Shimadzu, 7100 Riverwood Drive, Columbia, MD 21046 Join Restek for a day of science at Shimadzu’s upcoming Forensic Science Symposium. This event will consist of scientific talks from collaborators at a crime laboratory, police department, medical … Continue reading

Growing Analytical Solutions for Cannabis Testing: Innovative Products and Expertise for Fast, Accurate, Reliable Results (PDF 8494kB)

... of the technique used, lists of target compounds can be extensive, so column selectivity is an important factor to help you manage your everchanging analytical challenges every step of the way. Rxi GC COLUMNS...

Optimized RP-HPLC Method for Hydroxybenzoic Acids Balanced Retention for a Range of Polarities, Using an Ultra Aqueous C18 Column

... currently are available to the analyst, making column selection difficult. Although all alkyl phases column is compatible with 100% aqueous mobile phases. Ideal for samples that encompass...

ChromaBLOGraphy: The separation problem with CBC and CBD in GC analysis of medical cannabis with 5% phenyl-type columns.

In my post, “Don’t overestimate cannabidiol during medical cannabis potency determinations with gas chromatography. Use stationary phase selectivity for accuracy and hydrogen for fast analysis.”, I showed how an Rxi-35Sil MS GC column provides excellent separation for cannabichromene (CBC), cannabidiol (CBD), delta-8-THC, delta-9-THC, cannabigerol (CBG), and cannabinol (CBN).  I focused on the separation of cannabichromene [...]

PFAS LC Column Anatomy: Which Phase, Dimensions, and Particle Type Are Best?

... the development of better methods, and LC column selection is the foundation for building an improved approach analysis. Column Phase Selection The first decision to make when determining which PFAS ... methods is growing. But, when developing new analytical procedures, which PFAS LC column is best...

Selectivity in Gas Chromatography

..., single temperature scales of selectivity are of limited use for column selection . All chromatographers need a working knowledge of selectivity to facilitate the development ... of selectivity in gas chromatography. Dr. Poole has published numerous papers and books on the separation...

News: Separation Science 2012 Features Jaap de Zeeuw as Key Invited Speaker

We are proud to announce that Restek’s own Jaap de Zeeuw was invited to speak at Separation Science 2012 in Kuala Lumpur, Malaysia. Jaap joined chromatography experts from around the globe in presenting his work (see below for the abstract) … Continue reading

ChromaBLOGraphy: Restek at Separation Science meeting, Kuala Lumpur, Malaysia

Separation science was organized the 4th time in Kuala Lumpur Malaisia. The 2 days conference offered a practical program on developments in GC and LC with a lot of time to discuss applications while enjoying some of the local cuisine. Restek participated with a booth where displaying consumable products for GC, LC, and sample preparation(fig [...]

Sitting Down with a Chromatography Icon: Dr. Robert Sternberg

... columns of the GC-MS onboard the Huygens probe. After having been selected, we followed the same procedure ezGC has helped you optimize your GC conditions and column selection ... working with Restek, using our columns and Pro ezGC software on their missions, for over 15 years...

News: Restek® MXT® Columns and the Rosetta Spacecraft Make Headlines From Space

The European Space Agency (ESA) once again made the news when, awaking after 31 months of power-conserving hibernation, its Rosetta spacecraft sent a signal from beyond Jupiter to ESA’s mission control center in Germany. Already almost 500 million miles (800 … Continue reading

ChromaBLOGraphy: Air Columns – Part I: Why use a 60 m column for EPA Method TO-15?

Yes, the title of this blog is a question, because I have always wondered why a lot of air laboratories are using 60 m columns for U.S. EPA Method TO-15!?!?!? From everything I have seen, most laboratories are only analyzing what I refer to as the standard suite of 65 volatile organic compounds (VOCs) for […]

Totally Reliable Column-to-Column Performance

....8; standard deviation ±0.15). This narrow distribution ensures consistent column selectivity, which means Chromatographers today need assurances that every column they receive is going to perform ... Rxi columns demonstrate outstanding column-to-column reproducibility....

Comprehensive 2D Gas Chromatography — Making GC Separations Work Harder

.... For environmental analysis of PCBs, a carborane phase 2D column has been reported, where selectivity towards logical that, sooner or later, this coupled column technique will supplant the single-column method...

ChromaBLOGraphy: Fluorophenyl LC Phases- what you should know

They may be called PFP, PFPP, pentafluorophenyl, fluorophenyl or F5 phases. What they all have in common is the structure shown above (the pentafluorophenyl  group) attached to the silica particles. Most modern phases contain a propyl spacer between the above group and the silica, so that the phase looks something like this:     A […]

ChromaBLOGraphy: What happened to my GC column?

GC columns are commonly marketed for multiple methods/analyses, which is usually not an issue. Sometimes, however, when a column (or column set) has been used for one analysis, it may no longer work for another analysis. This is especially true when one analysis contains very dirty samples (or derivatized samples), and another contains very reactive [...]

Growing Analytical Solutions For Cannabis Testing Innovative Products And Expertise For Fast, Accurate, Reliable Results

... be extensive, so column selectivity is an important factor in achieving good separations. Both Raptor ARC-18 LC columns (Figure 5) and Rxi-5ms GC columns (Figure 6) provide the selectivity needed for accurate...

ChromaBLOGraphy: Cannabis Concentrates Part II: We’re Heading to Space!

Welcome back! It’s been a little while since my last blog, but in that time, we’ve been doing some interesting things regarding cannabis research. Last time, I discussed that we would be analyzing residual solvents in cannabis concentrates and today, I’m going to show one of the methods that we’ve been working on. If you […]

Optimizing the Analysis of Volatile Organic Compounds (PDF 2705kB)

....45mm ID and 0.53mm ID columns) 18 Narrow-bore Systems (0.18mm ID - 0.32mm ID columns) 21 Capillary Column Phases 21 Metal Columns22 Detection Systems23 Column Configurations 23 Detector Configurations23...

ChromaBLOGraphy: Let me help you schedule your technical journey at Pittcon 2011 this year.

Nearly 20,000 people attend Pittcon from over 90 countries and there is a staggering amount to see on the technical front. Luckily the show has the presentation titles on-line and easily searched in the PDF format. Simply enter in your search term and quickly find your area of interest. Below is an excel sheet that will [...]

ChromaBLOGraphy: Finally! A True Peak Capacity Increase for GCxGC

An ongoing controversy in comprehensive two-dimensional gas chromatography (GCxGC) is whether a true peak capacity increase has ever been achieved. You can read all about it in the article cited below, but in summary, Blumberg et. al state that a properly optimized one-dimensional (1D) GC system produces higher peak capacities than an improperly optimized GCxGC [...]

ChromaBLOGraphy: Dilute, Shoot, and Elute – am I missing anything? YES!

  Everyone’s lab is different in terms of how many samples per day are processed, but they all share the common pain point of sample preparation. Some samples like blood and plasma need a significant amount of prep to remove proteins, phospholipids, and salts, whereas labs running urine or drinking water samples can “get by” […]

ChromaBLOGraphy: GC compounds – poor peak shapes and missing peaks

One of the most common questions I am asked by customers is “Why is my peak shape so poor?” Another is “I do not see a peak for my compound, what should I do?” Sometimes they are developing a new method and have no experience with the compound(s) of interest; other times things worked well […]

Frequently Asked Questions: HPLC and UHPLC

... LC guard column selection guide. Back to top of page LC guard column selection guide. Back to top of page...

ChromaBLOGraphy: How to choose the correct GC column – Part 3

In this post, I will explain how one can use column capacity (along with detector selection) to help narrow down GC column choices even further. Before reading this post, I suggest you review Parts 1 & 2, which can be found in the links below. Part 1: http://blog.restek.com/?p=5426 Part 2: http://blog.restek.com/?p=5494   First, let’s talk [...]

News: Presentations Announced for AOAC 2012

The AOAC annual meeting is fast approaching. Beginning this Sunday, September 30th and running through next Wednesday, October 3rd, the 2012 AOAC meeting in Las Vegas, Nevada promises to be another exciting conference. The final program has been announced and … Continue reading

ChromaBLOGraphy: The main source of artificial trans fatty acids is banned on June 18, 2018. Are we ready?

This blog a part of a series: part 2, part 3, and part 4 One of the leading causes of deaths in the world is heart disease, accounting for approximately 24% of deaths in the USA in 2015.1 One of the factors linked to cardiovascular diseases is consumption of artificial trans fatty acids (TFAs). Artificial TFAs are […]

ChromaBLOGraphy: General (very general) guidelines to help meet GC detection limits.

Sometimes we (tech service) receive requests from customers who cannot meet detection limits when developing a new method or when trying to follow a current method. They ask for advice on how to meet these limits.  As a result, I decided to provide some (very) general guidelines that I would follow back-in-the-day when I would […]

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