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Dimethyldichlorosilane (DMDCS) Deactivating Agent

... reacts with active hydroxyl groups on the glass surface to produce a deactivated surface. A simple, step...

Deactivating Glassware with DMDCS Instruction Sheet (PDF 297kB)

... hydroxyl groups on glass surfaces, producing a deactivated surface. Note that the reaction releases...

Why Derivatize? Improve GC Separations with Derivatization (PDF 875kB)

... or carbohydrates. Alkylating reagents target active hydrogens on amines and acidic hydroxyl groups.3 Multiple of androsterone (Figure 1). Androsterone contains a hydroxyl group and a carbonyl group and exhibits poor peak...

Optimized RP-HPLC Method for Hydroxybenzoic Acids Balanced Retention for a Range of Polarities, Using an Ultra Aqueous C18 Column

... hydroxyl groups on the benzene ring (Figure 1). The additional hydroxyl groups’ varied positions with a carboxyl group (Figure 1). Hydroxybenzoic acids share the same basic structure, but contain additional ... Among hydroxybenzoic acids, hydroxyl groups on the benzene ring vary by position and number ... Ultra, aqueous c18, hydroxybenzoic acoids, hydroxyl groups, analgesics...

ChromaBLOGraphy: Simple Analysis of Bile Acids by GC-MS

Bile acids are a group of steroidal acids with carboxyl and hydroxyl groups on the side. They are the major metabolic products of cholesterol. Bile acids play important roles as biomarkers for early diagnosis and therapeutic monitoring of many diseases, especially liver and intestinal diseases. For instance, high levels of intestinal bile acids, in particular, […]

Rapid Analysis of 17 Bile Acids in Human Plasma by LC-MS/MS (PDF 767kB)

...) and mono-hydroxy bile acids (e.g., LCA). Because the position and stereochemistry of hydroxyl groups can bile acids in 6 minutes, including three isomer groups. Introduction Bile acids are a group of major...

Rapid Analysis of C3 Epimers of 25-Hydroxyvitamin D in Serum by LC-MS/MS (PDF 559kB)

... from the primary forms only in the configuration of the hydroxyl group in the third carbon position...

ChromaBLOGraphy: Improved Peak Symmetry as a Result of Derivatization of Phenols

Peak symmetry is important for determination and quantification of compounds. In order to create a symmetric peak, the compound must be able to elute all at more or less the same time. If a compound interacts with the column itself (ex: by temporarily adsorbing to the fused silica surface), it delays travel and effects peak […]

ChromaBLOGraphy: Analyzing for (4-Methylcyclohexyl)methanol (MCHM) in Source and Finished Drinking Waters

I got my hands on the new MCHM custom standard Chas blogged about Tuesday and performed some test runs yesterday. There are 2 isomers of the compound in the standard (cis and trans), referred to as isomer I and isomer II below because I don’t know the elution order. After injecting the 1 PPM standard […]

Rapid Analysis of C3 Epimers of 25-Hydroxyvitamin D in Serum by LC-MS/MS

.... Structurally, the C3 epimeric forms differ from the primary forms only in the configuration of the hydroxyl group in the third carbon position. Studies have shown that the C3 epimers of 25-hydroxyvitamin D have...

Rapid Analysis of 17 Bile Acids in Human Plasmaby LC-MS/MS

...) and mono-hydroxy bile acids (e.g., LCA). Because the position and stereochemistry of hydroxyl groups can minutes, including three isomer groups. Introduction Bile acids are a group of major ... including three isomer groups....

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