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Supplies & Accessories > HPLC Accessories

Browse our complete line of HPLC Accessories

HPLC & UHPLC Columns > HPLC & UHPLC Analytical Columns

Browse our complete line of HPLC & UHPLC Analytical Columns

Chromatography Columns > HPLC & UHPLC Columns

Browse our complete line of HPLC & UHPLC Columns

HPLC Accessories > Instrument Accessories & Parts

Browse our complete line of Instrument Accessories & Parts

LCLocker HPLC Organizer

...LCLocker HPLC Organizer ... Supplies & Accessories , HPLC Accessories , Lab Organizers ... Storage for ten HPLC columns (minimum 100 mm in length), compartments for accessories , and a book holder.* Small footprint—easily fits next to HPLC...

HPLC PEEK Performance Kit

...HPLC PEEK Performance Kit ... Supplies & Accessories , HPLC Accessories , Tools & Maintenance ... This HPLC PEEK Performance Kit is a useful complement to your HPLC system.Includes...

ASI Piston Seals

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts ... Over time the piston seal in every HPLC system becomes worn and must be replaced. Our piston ” and improves their wear characteristics. PTFE seals are more resistant to any HPLC solvent than UHMWPE seals...

PTFE Frits

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Piston

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Diaphragm

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Syringe

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Wash Seal

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Sapphire Pistons

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Rotor Seals

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Outlet Cap

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Connecting Tube

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Injector Lubricant

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Capillary Loop

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Sapphire Plungers

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Needle Seal

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Stator Assembly

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Plunger Assembly

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Sapphire Plungers

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Compression Screw

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Inlet Valve

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Needle Seat Assembly

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Bottle Head Assembly

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Active Inlet Cartridge

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Kel-F Washer

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Plunger Rinse Seal

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Inlet Check Valve Assembly

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Check Valve Cartridges

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Seal Wash Plunger Seal

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Sapphire Plunger Assembly Kit

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Ferrule

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Pump PM Kit

for Agilent 1050, 1100, 1200 HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Seal Wash Kit

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Survival Kit for HPLC, Stainless Steel

For start-up and maintenance in all HPLC systems.

...Kit includes: HPLC Capillary Tubing, SS, 1/16" x 0 .005" x 5 cm, 3-pk. HPLC Capillary Tubing, SS, 1/16" x 0 ... Survival Kit for HPLC, Stainless Steel ... Supplies & Accessories , HPLC Accessories , Tools & Maintenance ... necessary to set up and maintain your HPLC system: a selection of lengths and IDs of 1/16-inch tubing, plus...

Valves for HPLC

...Valves for HPLC ... Supplies & Accessories , HPLC Accessories , Valves...

HPLC Column Tubing

...HPLC Column Tubing ... Supplies & Accessories , HPLC Accessories , Column Hardware...

Prime/Purge Valve Repair Kit for Prime/Purge Valve

...Supplies & Accessories , HPLC Accessories , Valves...

PPS Hex-Head Fittings for HPLC

...PPS Hex-Head Fittings for HPLC ... Supplies & Accessories , HPLC Accessories , Tubing & Connections...

PEEK Hex-Head Fittings for HPLC

...PEEK Hex-Head Fittings for HPLC ... Supplies & Accessories , HPLC Accessories , Tubing & Connections...

Capillary Stainless Steel Tubing Assemblies

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Tubing & Connections ... of your Agilent HPLC systems. Meet or exceed manufacturer's performance. ...

PEEK-HT Hex-Head Fittings for HPLC and UHPLC

...PEEK-HT Hex-Head Fittings for HPLC and UHPLC ... Supplies & Accessories , HPLC Accessories , Tubing & Connections...

Rotor Seals

for Shimadzu LC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Outlet Check Valves

for Shimadzu LC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Lamps

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

ASI Replacement HPLC Pump Piston Rods

for Waters HPLC Systems

...ASI Replacement HPLC Pump Piston Rods ... Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts ... Buffer salts and metals from the HPLC system become imbedded on the surface of the piston seal...

ASI Replacement HPLC Pump Piston Rods

for Agilent HPLC Systems

...ASI Replacement HPLC Pump Piston Rods ... Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts ... Buffer salts and metals from the HPLC system become imbedded on the surface of the piston seal...

Needle Assembly

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Plunger Seals

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Deuterium Lamps

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Plunger Seals

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Piston Seals

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Inlet Check Valve Assembly

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Outlet Check Valve Assembly

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Back-up Seal

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Plunger Seal, Superseal

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Check Valve and Transducer Assembly

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Solvent Inlet Filter

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Stator Face Assembly

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Pump Maintenance Kit

for Agilent 1100, 1200 HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Seal, Gold Disk (outlet)

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Needle

for PerkinElmer HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Deuterium Lamps

for PerkinElmer HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Isolation Seal

for Waters HPLC Systems: Rheodyne Style

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Super Seal for Analytical Heads

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Head Plunger Seal Kits

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Insert Seal Parts Kit

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Inlet Manifold Kit

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Wash Face Seal

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Wash Tube Seal Kit

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Face Seals Replacement Kit

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Reference Valve Rebuild Kit

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Seal Pack Rebuild Kit (without Seal Wash Tube Assembly)

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Autosampler Preventive Maintenance Kit

for Agilent 1100, 1200 HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Outlet Check Valves

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Sieves for Outlet Valve

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Outlet Check Valve Assembly

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Rotor Seal Assembly, Rheodyne 7010

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Syringe Assembly, 250 µL

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Syringe, 500 µL

for Thermo HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Standard Pump Seal Kit

for PerkinElmer HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Outlet Cap & Gold Seal Assembly

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Preventive Maintenance (PM) Kits

for Waters HPLC & ACQUITY UPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Inlet Check Valve

for PerkinElmer HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Outlet Check Valve

for PerkinElmer HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

High Pressure Seal Kit

for PerkinElmer HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Standard High Pressure Seal

for PerkinElmer HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Low Pressure Seal

for PerkinElmer HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Vespel Rotor Seal

for Waters HPLC Systems: Rheodyne Style

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

PerformancePLUS Check Valve Cartridge

for Waters HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Seal Wash Kit, Binary Pump

for Agilent HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Roc Cyano HPLC Columns (USP L10)

...Roc HPLC columns are built to be the cornerstone for your LC lab. These solid, reliable columns are pressure rated for any 400 bar HPLC system and deliver the peak shape, reproducibility, ruggedness ... roc_01_ph_lc, structure_hplc_cyano.gif ... Roc Cyano HPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... HPLC column line. ... Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system. Solid and reliable—delivers the peak shape, reproducibility...

Roc Silica HPLC Columns (USP L3)

...Roc HPLC columns are built to be the cornerstone for your LC lab. These solid, reliable columns are pressure rated for any 400 bar HPLC system and deliver the peak shape, reproducibility, ruggedness ... roc_01_ph_lc, structure_hplc_silica.gif ... Roc Silica HPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... HPLC column line. ... Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system. Solid and reliable—delivers the peak shape, reproducibility...

Roc C18 HPLC Columns (USP L1)

...Roc HPLC columns are built to be the cornerstone for your LC lab. These solid, reliable columns are pressure rated for any 400 bar HPLC system and deliver the peak shape, reproducibility, ruggedness ... roc_01_ph_lc, structure_hplc_c18.gif ... Roc C18 HPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... HPLC column line. ... Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system. Solid and reliable—delivers the peak shape, reproducibility...

Roc C8 HPLC Columns (USP L7)

...Roc HPLC columns are built to be the cornerstone for your LC lab. These solid, reliable columns are pressure rated for any 400 bar HPLC system and deliver the peak shape, reproducibility, ruggedness ... roc_01_ph_lc, structure_hplc_c8.gif ... Roc C8 HPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... HPLC column line. ... Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system. Solid and reliable—delivers...

Roc Phenyl-Hexyl HPLC Columns (USP L11)

...Roc HPLC columns are built to be the cornerstone for your LC lab. These solid, reliable columns are pressure rated for any 400 bar HPLC system and deliver the peak shape, reproducibility, ruggedness ... roc_01_ph_lc, structure_hplc_phenyl-hexyl.gif ... Roc Phenyl-Hexyl HPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... HPLC column line. ... Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system. Solid and reliable—delivers the peak shape, reproducibility...

PEEK Unions, Tees, and Crosses for HPLC

...PEEK Unions, Tees, and Crosses for HPLC ... Supplies & Accessories , HPLC Accessories , Tubing & Connections ... Ideal for quickly and reliably connecting PEEK tubing in HPLC systems. One...

HPLC 30-Column Storage Cabinet

...Tired of stacks of HPLC columns on your lab benches? This easy-to-install cabinet saves space ... HPLC 30-Column Storage Cabinet ... Supplies & Accessories , HPLC Accessories , Lab Organizers...

Trident HPLC In-Line Guard Cartridge Holders

...Trident HPLC In-Line Guard Cartridge Holders ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... A Trident in-line guard cartridge holder can be used with almost any HPLC column by connecting...

HyperShear Static In-Line Mixers

...Supplies & Accessories , HPLC Accessories , Mobile Phase Accessories...

1/4" HPLC Frits

...1/4" HPLC Frits ... Supplies & Accessories , HPLC Accessories , Column Hardware...

LC Piston Seal Insertion Tool

...Supplies & Accessories , HPLC Accessories , Tools & Maintenance...

Plunger Seals

for Shimadzu LC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Survival Kit for HPLC, PEEK

For start-up and maintenance in all HPLC systems.

...Survival Kit for HPLC, PEEK ... Supplies & Accessories , HPLC Accessories , Tools & Maintenance ... essential for setting up and maintaining your HPLC system: PEEK tubing, connectors, and elbows, PTFE tubing...

1/16-Inch Stainless Steel Tube Fittings

...Supplies & Accessories , HPLC Accessories , Tubing & Connections ... Restek offers stainless steel tube fittings to help you maintain your HPLC equipment. Parker...

Restek Pack in a Box Kit

...Supplies & Accessories , HPLC Accessories , Column Hardware ... your own analytical HPLC columns is included (contents detailed below). The packing pump is electric...

Outlet Cap & Gold Seal Assembly Tool

for Agilent 1050, 1100 HPLC Systems

...Supplies & Accessories , HPLC Accessories , Tools & Maintenance...

Isolation Seal

for Agilent 1100, 1200 HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Suction Filter

for Shimadzu HPLC Systems

...Supplies & Accessories , HPLC Accessories , Instrument Accessories & Parts...

Ultra Silica HPLC Prep Columns

...lc_prep_columns, structure_hplc_silica.gif ... Ultra Silica HPLC Prep Columns ... Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra Cyano HPLC Prep Columns

...lc_prep_columns, structure_hplc_cyano.gif ... Ultra Cyano HPLC Prep Columns ... Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra IBD HPLC Prep Columns

...lc_prep_columns, structure_hplc_ibd.gif ... Ultra IBD HPLC Prep Columns ... Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Pinnacle II PAH HPLC Columns

... and reproducibility. If you are analyzing PAHs using HPLC, Pinnacle II PAH columns are a reliable, cost ... pinnacleii_co_hplc ... Pinnacle II PAH HPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Column End-Fittings

...Supplies & Accessories , HPLC Accessories , Column Hardware ... .0–4.6 mm ID HPLC columns. Fittings with flat bottom are intended for use on 1 .0–3.0 mm ID HPLC columns. ...

Ultra Quat Columns

...ultra_co_hplc ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Backpressure Regulators

...Supplies & Accessories , HPLC Accessories , Backpressure Regulators...

Ultra C18 HPLC Prep Columns

...lc_prep_columns, structure_hplc_c18.gif ... Ultra C18 HPLC Prep Columns ... Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra C8 HPLC Prep Columns

...lc_prep_columns, structure_hplc_c8.gif ... Ultra C8 HPLC Prep Columns ... Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra Biphenyl Columns (USP L11)

..., these columns are designed for selective and reliable HPLC applications.Since 2005, the Restek Biphenyl ... ultra_co_hplc, structure_hplc_biphenyl.gif ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra PFP Propyl Columns (USP L43)

..., these columns are designed for selective and reliable HPLC applications.The Restek PFP Propyl is a great ... ultra_co_hplc, structure_hplc_pfp_prop.gif ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

PFAS Delay Column

... the world. Because of their inertness, PFAS are used in tubing and other liquid-contacting parts of an HPLC , PFAS contamination from HPLC components can prevent accurate identification and quantitation of PFAS ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... -level analysis of PFAS in samples. Universal compatibility; works with: o Any HPLC or UHPLC up...

Waste Overflow Indicator for LC Systems

...Supplies & Accessories , HPLC Accessories , Mobile Phase Accessories...

Ultra Amino Columns (USP L8)

..., these columns are designed for selective and reliable HPLC applications.The general-purpose Restek Amino ... ultra_co_hplc, structure_hplc_amino.gif ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C1 Columns (USP L13)

..., these columns are designed for selective and reliable HPLC applications.This exceptionally stable C1 ... ultra_co_hplc, structure_hplc_c1.gif ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C4 Columns (USP L26)

..., these columns are designed for selective and reliable HPLC applications.Exceptionally stable C4 packing ... ultra_co_hplc, structure_hplc_c4.gif ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Silica Columns (USP L3)

..., these columns are designed for selective and reliable HPLC applications.Base-deactivated spherical silica ... ultra_co_hplc, structure_hplc_silica.gif ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc LC Guard Column Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... View the entire Roc HPLC column line....

Replacement PEEK Ferrules for Roc LC Guard Column Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... View the entire Roc HPLC column line....

Membrane Filters

...Supplies & Accessories , HPLC Accessories , Mobile Phase Accessories...

Graduated Safety-Coated Bottles

...Supplies & Accessories , HPLC Accessories , Mobile Phase Accessories...

Replacement Batteries for Waste Overflow Indicator for LC Systems

...Supplies & Accessories , HPLC Accessories , Mobile Phase Accessories...

Hub-Cap Filter Hose Barb

...Supplies & Accessories , HPLC Accessories , Mobile Phase Accessories...

PEEK Plug for Bottle Top Adaptors

...Supplies & Accessories , HPLC Accessories , Mobile Phase Accessories...

Book Holders

...Supplies & Accessories , HPLC Accessories , Lab Organizers...

Replacement Inserts for Tubing Dressing Tool

...Supplies & Accessories , HPLC Accessories , Tools & Maintenance...

Replacement Blade for Clean-Cut Tubing Cutter

...Supplies & Accessories , HPLC Accessories , Tools & Maintenance...

Tubing Clip

...Supplies & Accessories , HPLC Accessories , Tools & Maintenance...

Tubing Dressing Tool

...Supplies & Accessories , HPLC Accessories , Tools & Maintenance...

PEEK Fitting Extractor

...Supplies & Accessories , HPLC Accessories , Tools & Maintenance...

Roc HPLC Columns: The Reliable Cornerstone for Your LC Lab (PDF 2233kB)

...LC Columns NEW! Roc LC Columns: The Reliable Cornerstone for Your LC Lab Conventional HPLC alkyl chain Phenyl-Hexyl CH3 Si CH3 O Roc Family of LC Phases Roc LC Columns • Conventional HPLC ... Roc, HPLC...

USLC Columns—Ultra Selective Liquid Chromatography

Selectivity is the most influential factor affecting peak separation, or resolution. Restek Ultra Selective Liquid Chromatography (USLC) technology i...

Force LC Columns Brochure (PDF 4515kB)

...-lasting and reproducible—even with rapid cycling and elevated UHPLC pressures. • Fully scalable between HPLC and UHPLC productivity, and transfer methods across your entire lab, from your trusted-but-aged HPLC to the cutting ... Force LC columns, HPLC, UHPLC...

Force LC Columns Sales Sheet (PDF 3547kB)

...—even with rapid cycling and elevated UHPLC pressures. • Fully scalable between HPLC and UHPLC—easily transfer productivity, and transfer methods across your entire lab, from your trusted-but-aged HPLC to the cutting ... Force LC columns, HPLC, UHPLC...

Force LC Columns

Force LC columns are long-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure Satisfaction guarantee. And perhaps most important, Force LC columns are fully scalab...

Column Choice: A Critical Factor for Successful UHPLC Integration

... dominated by conventional HPLC systems. Column choice is a critical factor in successfully transferring methods between UHPLC and HPLC—poor choices ultimately cause failure or costly delays in development ... Column choice is a critical factor in successfully transferring methods between UHPLC and HPLC ... scaling methods, accuracy, method transfer, HPLC, UHPLC...

Roc HPLC Columns

Solid, reliable Roc HPLC columns deliver the peak shape, reproducibility, ruggedness, and performance you demand for all of your conventional HPLC applications. Available...

USLC Columns: Choose Columns Fast. Develop Methods Faster. (PDF 1815kB)

... method development. Figure 3: Restek offers the widest range of phase selectivities for HPLC and UHPLC μm HPLC * In addition to USLC stationary phases, Restek also offers C8, C18, and silica. Lit. Cat ... , method development, UHPLC, HPLC, USLC, reversed phase...

USLC Columns Put the Right Tools in Your LC Method Development Toolbox (PDF 1474kB)

... Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column significant selectivity differences, you can drastically speed up HPLC and UHPLC method development ... , reversed phase, H-S Model, method development, UHPLC, HPLC...

Restek HPLC Solvent Waste Overflow Indicator Instruction Sheet (PDF 311kB)

...Restek HPLC Solvent Waste Overflow Indicator cat.# 26543 Restek’s Waste Overflow Indicator is designed to notify the HPLC operator of a full waste bottle. An indicator alarm sounds in time to empty...

HPLC Fitting Removal Tool Instruction Sheet (PDF 57kB)

...HPLC Fitting Removal Tool Part #25325 Instructions for Use: Drill an opening approximately 1...

Restek HPLC Solvent Waste Overflow Indicator for GL45 Bottles Instruction Sheet (PDF 666kB)

...Restek HPLC Solvent Waste Overflow Indicator for GL45 Bottlescat.# 26550Restek’s Waste Overflow Indicator for G45 Bottles is designed to notify the HPLC operator ofafull waste bottle.An indicator alarm...

Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV (PDF 686kB)

...Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV Featured Application : Polycyclic Aromatic Hydrocarbons (PAHs) on a Pinnacle II PAH HPLC Column • Quick ~15-minute analysis of PAHs...

Single-Column Method for HPLC Analysis of Organic Acids in Fruit Juices, Using an Allure Organic Acids Column (PDF 636kB)

...#59530 HPLC Applications note Single-Column Method for HPLC Analysis of Organic Acids in Fruit for various components; and organic acid profile. High performance liquid chromatography (HPLC) is a powerful...

Trident Integral HPLC Guard Column System Instruction Sheet (PDF 395kB)

...Trident Integral HPLC Guard Column System The Trident system’s foundation consists Restek HPLC column with this configuration simply by adding a “-700” suffix to the catalog number...

4-Minute Bisphenol A (BPA) Analysis Increases Sample Throughput (PDF 402kB)

... separation regardless of instrument platform—compatible with both HPLC and UHPLC systems. Bisphenol A (BPA , ADHD, and other health conditions. BPA HPLC analysis can be done using a C18 LC column, but analysis...

USLC Columns Put the Right Tools in Your LC Method Development Toolbox Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection

..., you can drastically speed up HPLC and UHPLC method development by instead focusing on column choice selectivity. It has often been taught in HPLC method development that one can effectively alter selectivity ... , or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method development ... , "method development", USLC, "reversed phase", UHPLC, HPLC, "H-S Model"...

EXP and EXP2 Fittings: Reusable fittings for easy, reliable HPLC & UHPLC connections (PDF 694kB)

..., reliable HPLC & UHPLC connections www.restek.com • Patented ferrule can be installed repeatedly without , reliable HPLC & UHPLC connections for 10-32 fittings and 1/16" tubing PEEK End: Proven sealing ability...

ChromaBLOGraphy: Selectivity and the HPLC Mobile Phase

Stationary phase (i.e. column type) is a key contributor to selectivity in LC. But what about the mobile phase? A significant advantage, and sometimes challenge, in LC is that both the stationary and mobile phase chemistries can be modified to optimize our method. This contrasts with GC, where the main selectivity option is the stationary [...]

ChromaBLOGraphy: Building up pressure on HPLC?

We’ve all experienced a pressure error on HPLC at some time or another. Here are my best suggestions on handling them. Establish ground rules-This will help to define the problem and distinguish if there really is one. 1.     Check your programmed max pressure limit. Be aware of what the operating limit is for your system and [...]

EXP Fittings Instruction Sheet (PDF 707kB)

...EXP fittings Reusable fittings for easy, yet reliable HPLC & UHPLC connections for 10 the tubing into the female threaded port. 4. If using a hand-tight fitting for HPLC: Keep the tubing fully...

Outlet Cap and Gold Seal Assembly Tool Instruction Sheet (PDF 88kB)

... cat.# 25467 Outlet Cap and Gold Seal Assembly Tool for Agilent 1050 & 1100 HPLC Systems Easily...

ChromaBLOGraphy: Finding the right fitting for HPLC or UHPLC

If you are wondering which type of fitting you need, you can find a wealth of information in the Frequently Asked Questions (FAQs) section of our website, under the HPLC and UHPLC category. Click on the links below for answers to some common questions: What size threads are on the end fittings of my HPLC […]

Frequently Asked Questions: HPLC and UHPLC

... Method 8330B) by HPLC? Raptor LC Columns ? Hardware What size threads are on the end fittings of my HPLC column...

PEEK Secure-Fit Fittings Instruction Sheet (PDF 19kB)

... 1/16"-10/32 internal HPLC fitting connection port. Instructions for Use: Push the Secure...

Stainless Steel Secure-Fit Fittings Instruction Sheet (PDF 18kB)

.../16"-10/32 internal HPLC fitting connection port. Instructions for Use: Push the Secure-Fit fitting into the port...

ChromaBLOGraphy: [4]Troubleshooting HPLC- Fronting Peaks

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we have looked at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. Any time you have difficulty with poor peak shape, it is also […]

ChromaBLOGraphy: HPLC columns for the European Pharmacopoeia methods

European Pharmacopoeia (EU) methods tend to be vague in their description for column requirements, which can make choosing the correct column a little tricky.   To simply things, I listed the Restek HPLC columns that best match the columns (phases) listed in the European Pharmacopoeia “Reagents” chapter. Hopefully this makes it a little easier to […]

ChromaBLOGraphy: Analyze PAHs by HPLC without Acetonitrile

Labs that are trying to cope with the acetonitrile shortage are trying to reduce solvent usage, or going to an alternate mobile phase. One way to reduce solvent usage is to minimize your column volume. This means going to a narrower column and possibly a shorter column as well. Ultra high pressure liquid chromatography (UHPLC) [...]

Raptor SPP Core-Shell LC Columns

...ciency for drastically faster analysis times, better selectivity for substantially improved resolution, increased sample throughput with existing HPLC instrumentation, and long-lasting ruggedness for dependable reproducibility. Experience Selectivity Accelerated with this new species of column.

ChromaBLOGraphy: [3]Troubleshooting HPLC- Tailing Peaks

Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. If you are having difficulty with tailing, it is likely that you […]

HPLC Analysis of Glucosinolates in Vegetable Extracts without Ion Pairing Using an Ultra Aqueous C18 Column (PDF 675kB)

...Foods, Flavors & Fragrances Applications HPLC Analysis of Glucosinolates in Vegetable Extracts -performance liquid chromatography (HPLC). However, there are a number of glucosinolates with hydrophilic “R-” groups...

News: Our HPLC 2015 Page is Now Live

Visit www.restek.com/hplc2015 to learn more about our presentations, review technical abstracts, contact the presenters directly, and easily access the HPLC 2015 website.

News: Our HPLC 2018 Posters are Now Live

We have posted our HPLC 2018 presentations on our website for your review and study. Feel free to contact the authors directly with any questions you may have. www.restek.com/HPLC2018

News: Our HPLC 2019 Posters Are Now Live

We have uploaded our HPLC 2019 posters to our website for your review and study. Feel free to contact the authors directly with any questions you may have. www.restek.com/HPLC2019

News: Our HPLC 2016 Page is Now Live

      Before you go to HPLC 2016, visit Restek’s HPLC 2016 page to learn what we’ll be doing, to easily contact us with any questions you may have, and to access the meeting’s website for additional event details. www.restek.com/HPLC2016

Viva Wide Pore HPLC Columns Brochure (PDF 1015kB)

...Restek LC Viva Wide Pore HPLC Columns Exceptional Performance for Large Biomolecules! www -to-lot reproducibility. 2 www.restek.com 1-800-356-1688 or 1-814-353-1300 Viva Wide Pore HPLC Columns Conventional...

Simple, Reliable HPLC Analyses of Organic Acids Using Water-Compatible Allure or Ultra C18 Columns

... and authenticity. Reversed phase HPLC coupled with UV-Vis detection is a popular technique reversed phase HPLC using two C18 stationary phase columns in series. Because organic acids are low ... Commonly used organic acid methods (e.g. AOAC method 986.13) depend on reversed phase HPLC and C18...

Trident In-Line Filter and 1cm Guard Cartridge Holders Instruction Sheet (PDF 54kB)

... Cartridge Holders The Trident high-pressure in-line filter can be used with almost all HPLC columns can be used with almost all HPLC columns by connecting it with a short piece of 1/16" tubing...

News: Our HPLC 2015 Presentations Are Now Online

We have posted our HPLC 2015 presentations on our website for your review and study. Feel free to contact the authors directly with any questions you may have! www.restek.com/hplc2015

News: Our HPLC 2016 Presentations are Now Online!

      We have posted our HPLC 2016 presentations on our website for your review and study. Feel free to contact the authors directly with any questions you may have. www.restek.com/HPLC2016

News: Our HPLC 2018 Page is Now Live

Before you go to HPLC 2018, visit our conference page to learn what we’ll be doing, to easily contact us with any questions you may have, and to access the meeting’s website for additional event details. www.restek.com/HPLC2018

News: Our HPLC 2019 Page is Now Live

Before you go to HPLC 2019, visit our conference page to learn what we’ll be doing, to easily contact us with any questions you may have, and to access the conference’s website for additional event details. www.restek.com/HPLC2019

Roc LC Guard Column Holder for 10 mm Roc Guard Columns Instruction Sheet (PDF 268kB)

... holder can be used with all HPLC columns with standard 10-32 thread ports.* Install a Guard Column...

Optimized RP-HPLC Method for Hydroxybenzoic Acids Balanced Retention for a Range of Polarities, Using an Ultra Aqueous C18 Column

... these compounds represent such varying chemistry and polarity, finding an alkyl (C18) HPLC column that can Retention by Reversed Phase (RP) HPLC Many types of alkyl phases...

Dissecting Raptor LC Columns: A closer look at a new species (PDF 1725kB)

... with existing HPLC and UHPLC instrumentation. • Long-lasting ruggedness for dependable reproducibility . This benefit helps customers achieve faster separations with their existing HPLC instruments. Coupled...

ChromaBLOGraphy: Is it OK to use THF (tetrahydrofuran) on my HPLC?

Well, it might be. In the early days of HPLC, THF was commonly used as a mobile phase solvent. It has eluting strength similar to acetonitrile, but just slightly stronger. Since it technically is an ether and is very miscible with water, it is sometimes useful with reverse phase HPLC. It also provides additional options […]

Increase Data Quality for Sulfonamide Antibiotics Analysis by HPLC and UHPLC (PDF 975kB)

...Food Safety Applications Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC by a Biphenyl HPLC or UHPLC column can significantly improve data quality and reporting accuracy. Increase...

Featured Application: Polycyclic Aromatic Hydrocarbons (PAHs) on a Pinnacle II PAH HPLC Column Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV

... ~15-minute analysis of PAHs by HPLC provides fast results without the expense of UHPLC equipment the world. Many HPLC methods recommend a C18 column, but analysis times can be long (~30 minutes) and target ... for the analysis of PAHs by HPLC. These reliable columns provide baseline resolution of key PAHs in a fast ~15...

ChromaBLOGraphy: Troubleshooting HPLC- Loss in Response for All Analytes

[1]Troubleshooting HPLC- Loss in Response for all Analytes Just as in GC applications, patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those […]

Raptor SPP C18 Columns Sales Sheet (PDF 1888kB)

... columns to last longer, data to be reproducible, and existing HPLC and UHPLC instrumentation to run for drastically faster analysis times, especially for traditional HPLC instruments. - Faster analyses mean...

ChromaBLOGraphy: Can HPLC-UV Be Used For Terpenes Analysis In Cannabis?

While HPLC may be tempting to use for terpenes analysis, a GC/FID or GC/MS is really the most straightforward and recommended way of analyzing terpenes in cannabis. Terpenes, being relatively volatile and neutral, lend themselves rather nicely to GC in general. As you can see from Figure 1 below, coelutions of the cannabinoids and terpenes are very likely when […]

Analyze Carbamate Pesticides in a Fraction of the Time and Boost Productivity (PDF 787kB)

... formate in methanol Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument HPLC Peaks tR (min) Precursor usually are HPLC based. The U.S. EPA method for monitoring these compounds in drinking water, Method 531...

News: New Advice on an Old Topic: Buffers in Reversed-Phase HPLC

Author: Sharon Lupo and Ty Kahler Published By: LC-GC (chromatographyonline.com) Year of Publication: 2017 Link: http://www.chromatographyonline.com/new-advice-old-topic-buffers-reversed-phase-hplc Abstract: Buffers are commonly used in reversed-phase liquid chromatography (LC) to control the ionization state of analytes. However, the addition of buffers is much more complex than simple … Continue reading

Tech Tip — Why do I Lose Retention on my C18 in Highly Aqueous Mobile Phases?

... of a conventional C18, when analyzing polar compounds by HPLC....

ChromaBLOGraphy: HPLC Guard Cartridges and Extra-Column ‘Dead’ Volume

The benefit of using a guard column with dirty samples is clear. By adding a short sacrificial column before the analytical column, column life increases due to trapping of particulates and strongly-retained compounds. However, adding the guard cartridge does increase the extra-column volume of our flow path. And, extra volume leads to lower efficiency and [...]

News: New Roc HPLC Columns from Restek: The Reliable Cornerstone for Your LC Lab

Solid, reliable Roc HPLC columns are pressure rated for any 400 bar HPLC system and deliver the peak shape, reproducibility, ruggedness, and performance you demand for all of your conventional HPLC applications. An exceptional value for routine analyses and large-volume … Continue reading

ChromaBLOGraphy: What is Different about Prep LC? (Versus Analytical HPLC)

  The goal of prep LC is not to produce the best looking chromatogram. Prep LC can be used to purify a material for manufacturing or research purposes, or it can also be used for sample cleanup prior to analytical measurement, such as in gel permeation chromatography. Most Restek customers are more involved with using […]

ChromaBLOGraphy: Do reverse phase HPLC columns really need conditioning?

  Well, it’s partly a matter of semantics sometimes, but there is usually nothing drastic that you need to do to get your HPLC column going if it is new.  There is no baking at high temperatures involved like you might do for GC or that sort of thing. You do, however, need to equilibrate […]

ChromaBLOGraphy: [2]Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes

[2]Troubleshooting HPLC- Loss in Response for Some, but Not All Analytes Patterns in your HPLC chromatography can exhibit telltale signs that point toward probable sources of error.  In this brief series of posts, we will look at possible scenarios that you may encounter in the laboratory and how you might approach resolving those difficulties. When […]

ChromaBLOGraphy: What can I use to Qualify my HPLC instrumentation?

If you are setting up LC instrumentation in a new lab, you may find yourself looking for ways to perform Operational Qualification (OQ) tests. The requirements for qualifying a new instrument vary, depending on regulatory needs and SOP requirements, as well as the specific instrumentation. They might involve tests to confirm parameters such as solvent […]

Improve Characterization of Complex Protein Digests Using Viva Wide Pore HPLC Columns

... sample is chemically or enzymatically digested to produce peptides, HPLC/MS is used to resolve another, is the primary retention/separation mechanism. Viva Wide Pore HPLC Columns ... A Viva C18 wide pore HPLC column resolves a tryptic digest of bovine serum albumin primarily into 1...

ChromaBLOGraphy: Manual HPLC Valves, what we have and how they work

If you are looking for some manually activated valves to use with your HPLC system, we just might have what you are looking for. For a listing of these products, from our website, click here. For each of these, the flowpath is controlled manually via knobs on their exterior. We’ll briefly discuss some commonly used […]

Excellent LC-MS Separation of Penicillins and Cephalosporins Using Ultra IBD Columns (PDF 759kB)

.... Typically, HPLC columns can be used to analyze penicillins and their structurally related cephalosporins it the ideal choice for the analysis of penicillin- and cephalosporin-based antibiotics by HPLC or LC...

Easily Resolve Oxytocin PEGylation Reaction Products Using Viva Wide Pore HPLC Columns

... the therapeutic function of the active compound. Viva Wide Pore HPLC columns are ideal for the separation of large Aring; pores. Here we demonstrate the suitability of Viva Wide Pore HPLC columns for PEGylation reaction ... Viva Wide Pore HPLC columns are ideal for the separation of large molecules, as target analytes can...

Restek LC Columns and Accessories (PDF 17555kB)

... Overview: Premium, long-lasting, and reproducible columns for HPLC and UHPLC; fully scalable from 1.8 μm to 3 μm to 5 μm. Ideal for: Scaling methods between HPLC and UHPLC. Instruments supported: • Agilent...

Restek LC Guard Column Selector: How to Find the Right Guard Fast (PDF 2037kB)

... that pressure. If you’re employing traditional HPLC using a legacy column, you want a holder that houses and Analytical Column EXP Holders and Cartridges • Holder is compatible with all HPLC through UHPLC instruments...

EXP2 Fittings Instruction Sheet (PDF 217kB)

.... • Suitable for both HPLC and UHPLC. WARNING: Do not use EXP ferrules with standard nuts. Failure to use EXP...

ChromaBLOGraphy: Having Trouble With the ACN Shortage? Try MeOH for the Separation of Aldehydes and Ketones by HPLC

Many laboratories are struggling to find alternatives for HPLC separations that use acetonitrile for the organic. While there are other options, it can become difficult to find the right conditions, and method revalidation might also be required. The automotive industry commonly performs the analysis of DNPH-aldehydes and ketones using a custom-designed HPLC Column (Restek Allure-AK), [...]

Raptor ARC-18 LC Columns Sales Sheet (PDF 1872kB)

...: - Higher efficiencies and resolution for drastically faster analysis times, especially for traditional HPLC Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing Description qty. cat.# EXP Hand...

Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC Using Unique Biphenyl Column Selectivity

... by a Biphenyl HPLC or UHPLC column can significantly improve data quality and reporting accuracy effective HPLC separation of 11 common sulfonamides with complete resolution (Figure 1). Use of the Biphenyl...

Raptor FluoroPhenyl LC Columns Sales Sheet (PDF 1816kB)

... and resolution for drastically faster analysis times, especially for traditional HPLC instruments. - Faster ); Flow: 0.6 mL/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: HPLC. Column: Raptor Fluoro...

Restek Trident LC Column Protection System Instruction Sheet (PDF 330kB)

.... The Trident LC column protection system can be used with all HPLC columns with standard 10-32 ports* and has...

4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column

... in triplicate using a Shimadzu UFLCXR HPLC coupled with an AB SCIEX® API 4000 MS for this method is only 4.5 minutes using a 5 µm Ultra Biphenyl HPLC column. The fast analysis time and simple ... this dilute-and-shoot LC/MS/MS method which uses an Ultra Biphenyl HPLC column. Partial validation data...

News: Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection

Author(s): Rick Lake, Ty Kahler Published By: Restek Corporation Year of Publication: 2011 Link: http://www.restek.com/Technical-Resources/Technical-Library/Pharmaceutical/general_PHAN1348 Abstract: Column selectivity has the most significant influence on chromatographic peak separation, or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method … Continue reading

LC-MS/MS Analysis of Paraquat and Diquat without Ion-Pairing Reagents (PDF 530kB)

...M ammonium formate, 0.5% formic acid Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument HPLC Peaks t -42-5) Paraquat & Diquat Calibration Mix (2 components) Reference Standards EXP Reusable Fittings for HPLC...

Trace-Level Analysis of Melamine and Related Compounds by LC-MS/MS (PDF 763kB)

... (100%), Flow: 0.6 mL/min; Detector: MS/MS; Ion Mode: ESI+/ESI-; Mode: Scheduled MRM; Instrument: HPLC . EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing EXP Hand-Tight Fittings...

Simplified Methylmalonic Acid Analysis without Derivatization in Human Plasma by LC-MS/MS (PDF 584kB)

....5% Formic acid in methanol Detector MS/MS Ion Mode: ESIMode: MRM Instrument HPLC Notes 100 μL of sample ,700 psi. Apply Force LC columns to all of your HPLC and UHPLC instrument platforms at www...

Fast, Sensitive LC-MS/MS Analysis of GHB and Related Compounds in Human Blood for Forensic Testing (PDF 630kB)

...: 0.7 mL/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: HPLC; Notes: 100 μL of whole LC columns to all of your HPLC and UHPLC instrument platforms at www.restek.com/force Hybrid Ferrule...

News: Breathe New Life Into Your Old HPLC With Restek’s 5 μm Raptor™ Columns

With the advent of superficially porous particles (SPP or “core-shell” particles), the analytical community found the ability to gain far more efficiency and speed out of existing HPLC instrumentation. However, with these efficiency gains came higher pressures that exceeded the … Continue reading

Analysis of Nitrofurans in Honey Using LC/MS/MS and an Ultra C18 Column

... of 0.3ppb nitrofuran metabolites in honey are shown in Figure 1. The Ultra C18 HPLC column , such as steroids and vitamins. In analyses for nitrofuran antibiotics, an Ultra C18 HPLC column ... , is important in studying drug resistance and allergies in humans. The Ultra C18 HPLC column is an excellent...

News: Instrumentation Used for Terpene Analysis

Author: Tim Herring Restek Corporation Published By: Cannabis Industry Journal Year of Publication: 2017 Link: https://www.cannabisindustryjournal.com/column/instrumentation-used-for-terpene-analysis/ Abstract: Because terpenes are somewhat volatile, the Restek team recommends using gas chromatography and advises against using HPLC for terpene analysis. Many customers ask technical service which instrumentation … Continue reading

One Stop Shop for EPA Method 535 Reliably Analyze Acetamide Herbicide Degradates by LC/MS/MS

... Full package: reference standards, SPE cartridges, and HPLC columns (SPE) cartridges, and HPLC columns that meet method guidelines. In the procedure shown here, 6mL Carbo ... line of Method 535 products, including reference standards, solid phase extraction cartridges, and HPLC...

Malachite Green and Leucomalachite Green Analysis

... General Chromatographic Methodology Often reversed phase HPLC, typically . This can be circumvented by utilizing an oxidizing post column (e.g. lead oxide), in addition to the HPLC ... , where it is easily absorbed by fish tissue, and thus enters the human food supply. Reversed phase HPLC...

ChromaBLOGraphy: Live Long and Prosper-How to make your HPLC columns last longer

We frequently get questions about how long an HPLC or UHPLC column should last. To borrow a phrase from my colleagues, “Your mileage may vary.”  Columns are like cars, so their lifetime depends how they are used and how well they are maintained. That being said, some phases are known to be more rugged than [...]

Rapid Screening Method for Carbamates in Orange Oil Using an Ultra Carbamate HPLC Column

.... Carbamates are most easily determined via HPLC analysis because derivatization is required for GC analysis . The rapid screening method shown here uses the Ultra Carbamate HPLC column, which is designed specifically ... an easy, accurate screening method for carbamates in a complex matrix using an Ultra Carbamate HPLC column...

Raptor C18 LC Columns Brochure (PDF 2375kB)

... separations and speeding up analysis times with standard HPLC instruments. Raptor then evolved to bring profitability, you need columns to last longer, data to be reproducible, and existing HPLC instrumentation...

Raptor HILIC-Si: Simplify the Switch to HILIC (PDF 2013kB)

... for increasing sensitivity and selectivity in LC-MS analyses. • Fully compatible with both HPLC and UHPLC : 600 bar/8,700 psi Properties: • Compatible with both HPLC and UHPLC instruments. • Restek’s 2.7 μm...

Restek Essentials: Featured Chromatography Products 2019.1 (PDF 4834kB)

... porous particles that easily and fully scale from HPLC to UHPLC. Featuring the Time-Tested Restek -Free Fittings for Fast, Effortless HPLC and GC Connections EXP Nut EXP Ferrule 10-32 Female GC Capillary...

ChromaBLOGraphy: When should you use a buffer for HPLC, how does it work and which one to use?

As many of you know, buffers are usually used in mobile phases to hold the pH constant. They are composed of a weak acid or weak base, combined with its conjugate base or acid in solution that is at least partially aqueous (at least 50%).  This pair of compounds exists in equilibrium, so that when […]

Raptor FluoroPhenyl LC Columns Brochure (PDF 3042kB)

... selective USLC technology, improving separations and speeding up analysis times with standard HPLC ; Instrument: HPLC. Raptor reproducibility lets you develop assays with confidence. LC_CF0646 Raptor...

Raptor ARC-18 LC Columns Brochure (PDF 4942kB)

... technology, improving separations and speeding up analysis times with standard HPLC instruments. Raptor to bioanalytical work, whether you use traditional HPLC or UHPLC instruments, we’re all looking to simplify setup...

The Effects of LC Particle Choice on Column Performance: Switching from 3 and 5 µm Fully Porous Particles (FPP) to 5 µm Superficially Porous Particles (SPP) (PDF 559kB)

... 55 20 0.8 45 55 Detector DAD @ 254 nm Instrument HPLC Notes All columns were 150 mm x 4.6 mm ID Instrument HPLC Notes All columns were 50 mm x 2.1 mm ID. Peaks 1. Norfentanyl 2. Acetyl fentanyl 3...

Separating NSAIDs through Aromatic Selectivity Improve Retention by Using An Allure Biphenyl HPLC Column

... and, therefore, historically have been separated through reversed phase HPLC on C18 columns ... on our Allure Biphenyl HPLC column are based on pi-pi interactions, resulting in optimized retention...

Raptor Biphenyl LC Columns Sales Sheet (PDF 3731kB)

... times, especially for traditional HPLC instruments. - Faster analyses mean increased sample throughput Ferrule— ZDV Connections! Raptor EXP Guard Column Cartridges EXP Reusable Fittings for HPLC & UHPLC...

Fast, Accurate LC-MS/MS Analysis of the C3 Epimer of 25-Hydroxyvitamin D on Raptor FluoroPhenyl Columns (PDF 569kB)

... Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument HPLC LC_CF0644 Conc. Peaks MRM tR (ng/mL) 1. 25...

Improve Analysis of Aldehydes and Ketones in Air Samples with Faster, More Accurate Methodology (PDF 417kB)

... and analyzed by HPLC-UV. Typically, carbonyl LC methods require long analysis times (often over an hour are compatible with conventional 400 bar HPLC systems and benefit from the larger, low-clog frit used in Raptor...

All Matrix Interferences Resolved (PDF 1706kB)

... faster analysis times. - Increased sample throughput with existing HPLC instrumentation - Long -; Mode: MRM; Instrument: HPLC; Notes: The following reference standards were used: ethyl...

News: Eliminate the Impact of PFAS Instrument Contamination with a Restek PFAS Delay Column

Per-and polyfluorinated alkyl substances (PFAS) are often used in tubing and other liquid-contacting parts of an HPLC system, where they can leach into the mobile phase and interfere with sample analysis. This contamination is particularly problematic when analyzing at trace … Continue reading

Restek Advantage, 2011.1 (PDF 3826kB)

...•How to get faster analyses on any HPLC system•Rugged Rxi-5Sil MS column stands up to derivatization reagents and use it twice as many times. Fully Resolve Critical PAHs with an Optimized HPLC ColumnAlthough most...

Restek Essentials (PDF 1623kB)

.... Particelle interamente porose che possono adattarsi facilmente e interamente da HPLC a UHPLC. Con la fase utilizzare attrezzi per installare i fittings HPLC e GC, rapidamente e senza sforzo Dado EXP Ferrula EXP...

Resprep 12- & 24-Port Manifolds Instruction Sheet (PDF 517kB)

... sample filtration and will prevent sample debris from clogging HPLC instruments. Eluate collection...

Tech Tip: Column Conditioning Ensures Consistent EtG/EtS Results (PDF 402kB)

... Instrument HPLC Notes Reference Standards Ethyl-β-D-glucuronide (cat.# 34101) Ethyl-β-D-glucuronide-d5...

Definitive EtG/EtS LC-MS/MS Analysis: A Rugged 4-min Method for High-Throughput Labs (PDF 631kB)

... HPLC Notes Reference Standards Ethyl-β-D-glucuronide (cat.# 34101) Ethyl-β-D-glucuronide-d5 (cat Detector MS/MS Ion Mode: ESIMode: MRM Instrument HPLC Notes Reference Standards Ethyl...

ChromaBLOGraphy: Which LC Phase Do I Need?

So you’re thinking about doing HPLC (or UHPLC) and don’t have a method? You’ve never even seen a method for anything remotely similar. You might have even searched our HPLC chromatogram database for the application and haven’t found anything.  (After all, that’s the quickest and easiest way to get some ideas.) What to do… Let’s [...]

ChromaBLOGraphy: I have an Ultra Quat column and I know how to use it….

Are those words you wish you could say? Most recently, we seem to encounter more customers asking for advice in preparing a mobile phase for their Diquat and Paraquat analysis (related to EPA Method 549). There are a couple of different scenarios for this analysis. Please note that the procedures described here are intended for [...]

More of What You Need to Keep Your LC Flowing: Fittings, tubing, mobile phase management & safety, and more. (PDF 3764kB)

... Fittings Black Hex-Head 5,000 psi (350 bar) JR-58000-5 10-pk. 27714 PPS Hex-Head Fittings for HPLC • One -Head Fittings for HPLC • One-piece design; no ferrule required. • For 1/16" tubing on all 10-32 ports. • Made...

Thermal Desorption: A Practical Applications Guide: II. Residual Volatiles & Materials Emissions Testing (PDF 1445kB)

... followed by HPLC (ISO 16000-3, ASTM D5197, etc.). Paint - FLEC cell Background: The FLEC cell provides -GC-MS/FID or HPLC (for formaldehyde derivative) Reference: TDTS67 Micro-Chamber / Thermal Extractor (µ...

Featured Application: Fast Analysis of Bisphenol A on a Raptor Biphenyl LC Column 4-Minute Bisphenol A (BPA) Analysis Increases Sample Throughput

...—compatible with both HPLC and UHPLC systems. Bisphenol A (BPA) is a high , and other health conditions. BPA HPLC analysis can be done using a C18 LC column, but analysis times can...

The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses (PDF 878kB)

... and for their ability to help customers achieve faster separations with their existing HPLC instruments. With the launch -solid reliability to HPLC users for several years. Now their signature superficially porous particles, selective...

Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases (PDF 1244kB)

.... Centrifuge at 4,000 rpm for 5 minutes, then transfer 1 mL of the organic phase to a 1.5 mL HPLC vial and mix centrifugation at 4,000 rpm for 5 minutes, 1 mL of the organic phase was transferred to a 1.5 mL HPLC vial...

The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses

... for their selectivity and for their ability to help customers achieve faster separations with their existing HPLC -solid reliability to HPLC users for several years. Now, their signature superficially porous particles, selective ... and for their ability to help existing HPLC customers achieve faster separations without the need to purchase UHPLC...

ETP Active Film Multiplier for the HP/Agilent Technologies 5970 MSD Instruction Sheet (PDF 208kB)

...-sonic cleaner b. glass beaker (properly cleaned) c. Heptane (AR or HPLC grade) Under no circumstances...

Simplify Your Routine LC Maintenance with These Handy Kits for Agilent and Waters Systems (PDF 1109kB)

...-quality components in every kit. • Wide range of options for HPLC systems and pumps. www...

Simplify Sample Prep and Protect Analytical Equipment With Syringe Filters (PDF 484kB)

... & culture media — Nylon hydrophilic, low protein binding bases, HPLC solvents, alcohols, aromatic...

The Effects of LC Particle Choice on Column Performance: 2.7 vs. 5 µm Diameter Superficially Porous Particles (SPP) (PDF 539kB)

...M ammonium formate Detector MS/MS Ion Mode: ESI+ Instrument HPLC Notes Top: Raptor Biphenyl 50 mm x 2.1 mm...

ETP Active Film Multiplier for Shimadzu QP5000 MS Instruction Sheet (PDF 239kB)

... the following equipment; a.ultra-sonic cleaner b.glass beaker (properly cleaned) c.Heptane (AR or HPLC grade...

10 Critical Steps to World-Class Reference Standards (PDF 3190kB)

... of the following techniques: GC-FID, HPLC, GC-ECD, GC-MS, LC-MS, refractive index, and melting point. Calibrate...

The Institute for Nutraceutical Advancement (INA) Validates GC Methods for Saw Palmetto Using Rtx-5 and Stabilwax Columns (PDF 650kB)

... many use high performance liquid chromatography (HPLC), two methods involve gas chromatography (GC...

How do intrinsically base-deactivated phases work?

... in the production of HPLC columns, mainly because it is well-suited to high-pressure chromatographic separations ... in the production of HPLC columns, mainly because it is well-suited to high-pressure chromatographic separations...

Fast, Accurate LC-MS/MS Method for Folate Deficiency Biomarkers in Plasma (PDF 513kB)

.../Ferrule) 10-pk. 25938 EXP Hand-Tight Nut (w/o Ferrule) ea. 25939 EXP Reusable Fittings for HPLC & UHPLC...

ChromaBLOGraphy: What is SPP and when should I use a Raptor column?

While this sounds like a very broad question, I’m sure it is crossing some of your minds. To start out, SPP stands for superficially porous particles. It has also been called “core-shell”.   If you are a veteran in the world of HPLC, this term is old hat for you. If you are new to the […]

Diagnosing and Preventing High Back Pressure Problems in LC Systems (PDF 5850kB)

...’s a fact of life for HPLC and UHPLC analyses. The force that is necessary to push a liquid mobile phase of large changes in mobile phase composition. In all cases, be sure to only use HPLC-grade chemicals...

ChromaBLOGraphy: A C18 is a C18, right?

    Sorry, that actually is not true. Although many methods only list the USP code L1 for all C18 HPLC columns, there are many different varieties of C18 columns available from Restek. Here are some of the ways they may differ:   Differences in Silica Depending on the silica particle that the C18 phase […]

ChromaBLOGraphy: Technical Service “Red Flags” – LC

This post is an extension of a series of posts pertaining to “Red Flags” my colleague has written, pertaining to GC analysis. These are situations and symptoms that tell us in the Tech Service group that something is just not right. So what are examples of some “Red Flags” that we commonly see for HPLC?  […]

Grace Chromatography Consumables Cross Reference

Are you looking for an alternate source for chromatography products similar to those you once ordered from W. R. Grace? Restek has you covered with a...

News: 4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column

Author(s): Amanda Rigdon Published By: Restek Corporation Year of Publication: 2011 Link: http://www.restek.com/Technical-Resources/Technical-Library/Clinical-Forensic-Toxicology/cf_an1343 Abstract: Sample throughput for benzodiazepines in urine and whole blood can be increased by adopting this dilute-and-shoot LC/MS/MS method which uses an Ultra Biphenyl HPLC column. Partial … Continue reading

ChromaBLOGraphy: Selecting a Detector for LC

Although a blog is not a good way to teach all there is to know about LC detectors, I have tried to put together information to give an overview of the most common detectors.  Restek does not sell HPLC instruments or detectors, but we think we might be able to help if you are very […]

ChromaBLOGraphy: Choosing a guard cartridge holder for LC

If you need help choosing one of the Trident guard cartridge holders, check out our information found in the HPLC and UHPLC FAQs section of our website. The FAQs contain a lot of good information and are often overlooked. For most of our columns we prefer the Trident Direct Guard holder system, because of its […]

News: Restek Unleashes Superior Raptor Performance on Your UHPLC Analyses — Now with 1.8 μm Particles

Raptor LC columns have been providing higher efficiencies, faster analyses, and rock-solid reliability to HPLC users for several years. And they have just evolved again. Restek has brought the benefits of Raptor columns to those who have made the leap … Continue reading

Novel Column Chemistry—High Impact, Low Cost Technology

...The three areas of development in HPLC that are most significant to the pharmaceutical /MS), and the development of novel column chemistries. These three factors have invigorated the HPLC market because...

Thomson SINGLE StEP Filter Vials (PDF 738kB)

... of buffers & culture media — Nylon hydrophilic, low protein binding bases, HPLC solvents, alcohols...

Highly Selective LC-MS/MS Analysis of Mycotoxins from Multiple Classes (PDF 556kB)

...-Turn, and the Opti- prefix are registered trademarks of Optimize Technologies, Inc. EXP Reusable Fittings for HPLC...

High-Throughput Analysis of Cannabinoids by LC-UV (PDF 1054kB)

...; Instrument: HPLC Peaks tR (min) 1. Cannabidivarinic acid (CBDVA) 1.877 2. Cannabidivarin (CBDV) 2.086 3...

Analysis of d- and l-Amphetamine and Methamphetamine Enantiomers for High-Throughput Labs (PDF 568kB)

....5 mL/min; Detector: MS/MS; Ion Mode:ESI-; Mode: MRM; Instrument: HPLC; Notes: A 500 ng/mL standard...

ChromaBLOGraphy: Resurrecting an old reverse phase LC column- should I, could I, how would I???

Well, if we had an Easter egg hunt and you found an egg that was from last year or the year before, you could probably tell the difference and you would know pretty quickly if it had gone bad.  Let’s just say that is not so easy with HPLC columns. If you’ve dealt with this […]

ChromaBLOGraphy: What can I use to filter my mobile phase?

If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed […]

Raptor Biphenyl LC Columns Brochure (PDF 6071kB)

... selective USLC technology, improving separations and speeding up analysis times with standard HPLC using SPE, SLE, or other extensive sample preparations. EXP Reusable Fittings for HPLC & UHPLC for 10...

ChromaBLOGraphy: How much buffer to weigh and what’s the best way to prepare my buffered mobile phase?

Occasionally we get this question and sometimes folks do need help with the calculations. We discussed in a previous post what a buffer is composed of. Click on this link if you need to review it: When should you use a buffer for HPLC, how does it work and which one to use? For a […]

4.5 Minute Analysis of Benzodiazepines in Urine and Whole Blood Using LC/MS/MS and an Ultra Biphenyl Column (PDF 672kB)

... using a Shimadzu UFLCXR HPLC coupled with an AB SCIEX® API 4000 MS/MS operating in positive electrospray .5 minutes using a 5 μm Ultra Biphenyl HPLC column. The fast analysis time and simple dilute...

For advanced chromatogram searching, please check out our Chromatogram Library Search Tool.

Antipsychotics and Antidepressants in Human Urine on Raptor Biphenyl by LC-MS/MS

LC_CF0749

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Parabens on Raptor C18

LC_PH0531

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SRM 870 Test Mix on Viva C18

LC_EX0388

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