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HPLC & UHPLC Columns > HPLC & UHPLC Analytical Columns

Browse our complete line of HPLC & UHPLC Analytical Columns

Chromatography Columns > HPLC & UHPLC Columns

Browse our complete line of HPLC & UHPLC Columns

Replacement Filters for UltraLine UHPLC In-Line Filter

...Replacement Filters for UltraLine UHPLC In-Line Filter ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Pinnacle DB PAH UHPLC Columns

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Pinnacle DB PAH UHPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Stainless Steel Fittings for UHPLC

...Stainless Steel Fittings for UHPLC ... Ideal for connecting stainless steel tubing in UHPLC systems.For 1/16" tubing...

UltraLine UHPLC In-Line Filter

...UltraLine UHPLC In-Line Filter ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... -effective protection for UHPLC systems. Reliable way to extend column lifetime. Leaktight to 15...

Pinnacle DB IBD UHPLC Columns (USP L68)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Pinnacle DB IBD UHPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

PEEK-HT Hex-Head Fittings for HPLC and UHPLC

...PEEK-HT Hex-Head Fittings for HPLC and UHPLC...

UltraShield UHPLC PreColumn Filter

...UltraShield UHPLC PreColumn Filter ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... Cost-effective protection for UHPLC systems.Reliable way to filter out particulates and extend column lifetime.Minimize extra column volume and maximize UHPLC sample...

Stainless Steel Unions, Tees, and Crosses for UHPLC

...Stainless Steel Unions, Tees, and Crosses for UHPLC ... Ideal for quickly and reliably connecting stainless steel tubing in UHPLC systems...

Pinnacle DB Cyano Columns (USP L10)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

PEEK Tips for Trident Direct Guard Cartridge Systems

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Replacement Trident PEEK Ferrules

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

PFAS Delay Column

... be used with any type of HPLC or UHPLC system up to 15,000 psi (1034 bar) and with any analytical column ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... -level analysis of PFAS in samples. Universal compatibility; works with Any HPLC or UHPLC up...

Pinnacle DB C18 Columns (USP L1)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB C8 Columns (USP L7)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB Silica Columns (USP L3)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB Biphenyl Columns (USP L11)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB PFP Propyl Columns (USP L43)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB Aqueous C18 Columns (USP L1)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Titanium Hybrid Replacement Ferrules

...+ bar)—no tools needed! For a reliable 20,000+ psi (1400+ bar) UHPLC connection with either fitting instructions may result in unsafe UHPLC connections and/or non-ZDV connections.Intellectual Property ... EXP Reusable Fittings for HPLC & UHPLC ... : Reusable fittings for easy, reliable HPLC and UHPLC connections. ... + psi seal. Both hand-tight and hex-head styles wrench tighten for reliable UHPLC use up to 20...

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Hand-Tight Fittings

...+ bar)—no tools needed! For a reliable 20,000+ psi (1400+ bar) UHPLC connection with either fitting instructions may result in unsafe UHPLC connections and/or non-ZDV connections.Intellectual Property ... EXP Reusable Fittings for HPLC & UHPLC ... : Reusable fittings for easy, reliable HPLC and UHPLC connections. ... + psi seal. Both hand-tight and hex-head styles wrench tighten for reliable UHPLC use up to 20...

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Hex-Head Fittings

...+ bar)—no tools needed! For a reliable 20,000+ psi (1400+ bar) UHPLC connection with either fitting instructions may result in unsafe UHPLC connections and/or non-ZDV connections.Intellectual Property ... EXP Reusable Fittings for HPLC & UHPLC ... : Reusable fittings for easy, reliable HPLC and UHPLC connections. ... + psi seal. Both hand-tight and hex-head styles wrench tighten for reliable UHPLC use up to 20...

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Hand-Tight Coupler

...+ bar)—no tools needed! For a reliable 20,000+ psi (1400+ bar) UHPLC connection with either fitting instructions may result in unsafe UHPLC connections and/or non-ZDV connections.Intellectual Property ... EXP Reusable Fittings for HPLC & UHPLC ... : Reusable fittings for easy, reliable HPLC and UHPLC connections. ... + psi seal. Both hand-tight and hex-head styles wrench tighten for reliable UHPLC use up to 20...

EXP2 Reusable Fittings for HPLC & UHPLC

for 10-32 fittings and 1/16" tubing

... nuts. Failure to use EXP fittings according to the included instructions may result in unsafe UHPLC ... EXP2 Reusable Fittings for HPLC & UHPLC ... Fittings: Reusable fittings for easy, reliable HPLC and UHPLC connections. ... vendor-specific tubing is not required. Suitable for both HPLC and UHPLC. *Rated...

Pinnacle DB Guard Cartridges

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Raptor HILIC-Si LC Columns (USP L3)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns ) Properties: Compatible with both HPLC and UHPLC instruments. Restek’s 2.7 µm core ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... .Fully compatible with both HPLC and UHPLC when using low back-pressure HILIC mobile phases...

Force FluoroPhenyl LC Columns (USP L43)

...-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure and 1.8 µm UHPLC, so you can easily transfer and optimize methods without extensive calculations. Apply ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Force Biphenyl LC Columns (USP L11)

...-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure and 1.8 µm UHPLC, so you can easily transfer and optimize methods without extensive calculations. Apply ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Restek Pack in a Box Kit

...Chromatography Columns , HPLC & UHPLC Columns , Bulk Packing Materials...

Roc LC Guard Column Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Roc LC Guard Column Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Replacement PEEK Ferrules for Roc LC Guard Column Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Pinnacle II PAH Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Allure Silica Columns (USP L3)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Allure Acrylamide LC Column

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Amino Columns (USP L8)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C1 Columns (USP L13)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C4 Columns (USP L26)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Cyano Columns (USP L10)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Silica Columns (USP L3)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Carbamate Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Allure Organic Acids Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Quat Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Allure AK Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle II PAH HPLC Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor EtG/EtS LC Column

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Force EXP Guard Column Cartridges

...-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure and 1.8 µm UHPLC, so you can easily transfer and optimize methods without extensive calculations. Apply ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... columns to all of your HPLC and UHPLC instrument platforms at www.restek.com/force...

Force C18 LC Columns (USP L1)

...-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure and 1.8 µm UHPLC, so you can easily transfer and optimize methods without extensive calculations. Apply ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Bulk Packing Materials (5 µm)

...Chromatography Columns , HPLC & UHPLC Columns , Bulk Packing Materials...

High-Pressure Frit-Type In-Line Filter

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Replacement Cap Frit Filters for Trident Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Trident HPLC In-Line Guard Cartridge Holders

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Viva Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Allure Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Ultra Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Ultra Aromax Columns (USP L11)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc C8 HPLC Columns (USP L7)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc C18 HPLC Columns (USP L1)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc Phenyl-Hexyl HPLC Columns (USP L11)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc Cyano HPLC Columns (USP L10)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc Silica HPLC Columns (USP L3)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C8 Columns (USP L7)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C18 Columns (USP L1)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Aqueous C18 Columns (USP L1)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra IBD Columns (USP L68)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva Silica Columns (USP L3)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva C18 Columns (USP L1)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva C8 Columns (USP L7)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva C4 Columns (USP L26)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva PFP Propyl Columns (USP L43)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva Biphenyl Columns (USP L11)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Biphenyl Columns (USP L11)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra PFP Propyl Columns (USP L43)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor EXP Guard Column Cartridges

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns .Maximum cartridge pressure: 1034 bar/15,000 psi* (UHPLC), 600 bar/8700 psi (2.7 μm); 400 bar/5800 psi (5 ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Raptor Polar X LC Columns

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... -shell particles provide UHPLC-like speed and efficiency on all makes and models of LC systems. Ideal...

EXP In-Line Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... repeatedly without compromising high-pressure seal. Connects to any HPLC, UHPLC, injection...

Raptor Biphenyl LC Columns (USP L11)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor ARC-18 LC Columns (USP L1)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor FluoroPhenyl LC Columns (USP L43)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor C18 LC Columns (USP L1)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns profitability, you need columns to last longer, data to be reproducible, and existing HPLC and UHPLC ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Silica HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra C18 HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra C8 HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra Cyano HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra IBD HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra Biphenyl Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra PFP Propyl Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

EXP Direct Connect Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Ultra Aqueous C18 HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Trident LC Column Protection System

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Stainless Steel Tubing

...Ideal for all plumbing in UHPLC systems.1/16" OD in 3- and 10-meter coiled...

Flexible Premium Grade Stainless Steel Tubing

...Ideal for all plumbing in UHPLC systems.1/32" OD tubing with 17 mm long, 1...

Thomson SINGLE StEP Low Evaporation Filter Vials

..., including UHPLC instruments. ...

PAH Interferences Standard (5 components)

... with Pinnacle II PAH columns or UHPLC compatible on the Pinnacle DB PAH column....

Thomson SINGLE StEP Standard Filter Vials

..., including UHPLC instruments....

Thomson SINGLE StEP eXtreme Filter Vials

... capacity and low dead volume (120 μL). Fit most standard 12 x 32 mm autosamplers, including UHPLC...

Thomson SINGLE StEP Nano Filter Vials

... low dead volume (8 μL). Fit most standard 12 x 32 mm autosamplers, including UHPLC...

EU 15+1 PAH Standard (16 components)

... columns or UHPLC compatible on the Pinnacle DB PAH column....

OPTI-MAX Check Valves

... with the OPTI-MAX check valve system.*UHPLC cartridges rated to 20,000 psi (1400 bar)Intellectual...

UHPLC Filters: Protect Your Column and Chromatographic Performance (PDF 476kB)

...UHPLC Filters Protect Your Column and Chromatographic Performance UltraShield UHPLC Precolumn Filter • Economical protection against microparticles. • Extend UHPLC column lifetime. • Negligible ... precolumn filter, UltraShield, UltraLine, UHPLC...

The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses (PDF 878kB)

... Particles (SPP) for UHPLC Analyses By Scott Grossman, Susan Steinike, Dan Li, and Justin Steimling of Raptor 1.8 μm columns, this superior Raptor performance has been unleashed on your UHPLC analyses. But ... 1.8, Raptor, UHPLC...

Force LC Columns Sales Sheet (PDF 3547kB)

...—even with rapid cycling and elevated UHPLC pressures. • Fully scalable between HPLC and UHPLC—easily transfer -edge UHPLC you just plumbed last week. This incredibly rugged and supremely consistent column line ... Force LC columns, HPLC, UHPLC...

EXP and EXP2 Fittings: Reusable fittings for easy, reliable HPLC & UHPLC connections (PDF 694kB)

..., reliable HPLC & UHPLC connections www.restek.com • Patented ferrule can be installed repeatedly without , reliable HPLC & UHPLC connections for 10-32 fittings and 1/16" tubing PEEK End: Proven sealing ability...

UltraShield UHPLC Precolumn Filter Instruction Sheet (PDF 161kB)

...UltraShield UHPLC Precolumn Filter cat.# 24995, 24996, 24997, 25809, 25810, 25811 Ultra Shield precolumn filters are designed to be an affordable UHPLC precolumn filter with a total volume of less than...

The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses

... on your UHPLC analyses. But, what exactly does that mean? In this technical note, we will explore the benefits of using Raptor 1.8 µm LC columns in your UHPLC instrument with a focus on peak capacity, column ... and for their ability to help existing HPLC customers achieve faster separations without the need to purchase UHPLC on your UHPLC analyses. But, what exactly does that mean? In this technical note, we will explore ... Raptor, 1.8, UHPLC...

EXP Fittings Instruction Sheet (PDF 310kB)

...Reusable fittings for easy, yet reliable HPLC & UHPLC connections for 10-32 fittings and 1 + bar)—no tools needed! For a reliable 20,000+ psi (1400+ bar) UHPLC connection with either fitting...

Dissecting Raptor LC Columns: A closer look at a new species (PDF 1725kB)

... with existing HPLC and UHPLC instrumentation. • Long-lasting ruggedness for dependable reproducibility with small-particle technology, SPP also provides an efficiency boost to those running UHPLC when compared...

EXP2 Fittings Instruction Sheet (PDF 336kB)

... mean vendor-specific tubing is not required. • Suitable for both HPLC and UHPLC. For replacement .optimizetech.comI-03945D | 20200422INNOVATIVE The UHPLC Connection1. Insert the EXP uni-directional cartridge into holder...

Force LC Columns

Force LC columns are long-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure Satisfaction guarantee. And perhaps most important, Force LC columns are fully scala...

Restek Essentials: Featured Chromatography Products 2020.2 (PDF 7897kB)

... and learn more at www.restek.com/LongLifeESI • Cost-effective protection for UHPLC systems. • Minimize . Reliably Filter Out Particulates and Extend UHPLC Column Lifetime with UltraShield UHPLC Pre...

Raptor SPP C18 Columns Sales Sheet (PDF 1888kB)

... columns to last longer, data to be reproducible, and existing HPLC and UHPLC instrumentation to run % B), 3.50 min (20% B); Flow: 1.0 mL/min; Detector: PDA @ 254 nm; Instrument: UHPLC. LC_PH0532...

Restek LC Guard Column Selector: How to Find the Right Guard Fast (PDF 2037kB)

... families and are intended for a specific technique. For example, if you’re performing UHPLC, you are using and Analytical Column EXP Holders and Cartridges • Holder is compatible with all HPLC through UHPLC instruments...

Fast, Simple LC-MS/MS Analysis of Creatine and Creatinine (PDF 568kB)

...Shield UHPLC precolumn filter 0.2 μm (cat.# 25810); Temp.: 40 °C; Sample: Diluent: 20:80 Water ; Instrument: UHPLC; Notes: Sample Preparation: Endogenous levels of creatinine and creatine in human plasma...

High-Throughput LC-MS/MS Analysis of Phosphatidylethanol in Whole Blood (PDF 561kB)

...:1 Methanol:2-propanol Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC Notes Sample Preparation .6 mm cat.# Raptor FluoroPhenyl EXP Guard Column Cartridge UHPLC 3-pk. 9319U0252 9319U0253 Raptor...

Frequently Asked Questions: HPLC and UHPLC

...? Is there a Restek guard cartridge to go with my UHPLC column? Which guard cartridge polymer-based fittings? Which fittings can be used for UHPLC...

ChromaBLOGraphy: Finding the right fitting for HPLC or UHPLC

If you are wondering which type of fitting you need, you can find a wealth of information in the Frequently Asked Questions (FAQs) section of our website, under the HPLC and UHPLC category. Click on the links below for answers to some common questions: What size threads are on the end fittings of my HPLC […]

Raptor ARC-18 LC Columns Sales Sheet (PDF 1872kB)

... UltraShield UHPLC PreColumn Filter—for 1.8 μm Raptor Columns Description Filter Porosity qty. cat .# UltraShield UHPLC PreColumn Filter 0.2 μm frit ea. 25809 5-pk. 25810 10-pk. 25811 EXP Reusable...

EXP In-Line Holder Instruction Sheet (PDF 355kB)

...EXP In-Line Holder EXP® AND EXP®2 IN-LINE HOLDER The UHPLC Connection1. Insert the EXP uni...

Raptor FluoroPhenyl LC Columns Sales Sheet (PDF 1816kB)

...L/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC. Column: Raptor FluoroPhenyl (cat.# 9319A1E L/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC. Easily separate compounds that coelute...

4-Minute Bisphenol A (BPA) Analysis Increases Sample Throughput (PDF 402kB)

... separation regardless of instrument platform—compatible with both HPLC and UHPLC systems. Bisphenol A (BPA . In addition, Raptor Biphenyl columns can be used with both conventional HPLC and UHPLC instruments. For BPA...

EXP Direct Connect Holder Instruction Sheet (PDF 373kB)

... The UHPLC Connection1. Insert the EXP uni-directional cartridge into holder body.holder capholder body2 .03945D | 20200422INNOVATIVE The UHPLC Connection1. Insert the EXP uni-directional cartridge into holder...

Raptor FluoroPhenyl LC Columns Brochure (PDF 3042kB)

... instruments. Raptor then evolved to bring that same improved speed, efficiency, and selectivity to UHPLC L/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC. Peaks tR (min) Precursor Ion Product Ion 1...

Raptor LC Columns: Stationary Phase: EtG/EtS (PDF 1487kB)

...G/EtS (cat.# 9325A12); Dimensions: 100 mm x 2.1 mm ID, Particle Size: 2.7 μm; Guard Column: UltraShield UHPLC .# 2.7 μm 2.1 mm 100 mm ea. 9325A12 UltraShield UHPLC PreColumn Filter • Cost...

Rapid Analysis of 17 Bile Acids in Human Plasma by LC-MS/MS (PDF 767kB)

... minimum resolution of isomers. In this study, a simple UHPLC method was developed for the simultaneous on a Shimadzu Nexera UHPLC paired with a Shimadzu LCMS- 8060 MS/MS. Instrument conditions were as follows...

Raptor Biphenyl LC Columns Sales Sheet (PDF 3731kB)

... Ferrule— ZDV Connections! Raptor EXP Guard Column Cartridges EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing EXP Direct Connect Holder UltraShield UHPLC PreColumn Filter—for 1...

Raptor Biphenyl LC Columns Brochure (PDF 6071kB)

... instruments. Raptor then evolved to bring that same improved speed, efficiency, and selectivity to UHPLC are rugged enough to last well past 1,000 injections, even in the high-pressure conditions of UHPLC. Column...

Direct Analysis of Morphine, M3G and M6G Metabolites, and Related Compounds in Urine by LC-MS/MS (PDF 778kB)

... compounds was conducted on a Shimadzu Nexera UHPLC equipped with a SCIEX API 4500 MS/MS. Detection equipped with an UltraShield UHPLC precolumn filter with a 0.2 μm frit. Instrument conditions are detailed...

Raptor C18 LC Columns Brochure (PDF 2375kB)

... that same improved speed, efficiency, and selectivity to UHPLC analyses by offering 1.8 μm particle columns % B), 3.50 min (20% B); Flow: 1.0 mL/min; Detector: PDA @ 254 nm; Instrument: UHPLC. LC_PH0532...

Reliable HILIC LC-MS/MS Analysis of 4-Methylimidazole (4-MEI) on Raptor FluoroPhenyl Columns (PDF 1191kB)

... acid in acetonitrile Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC LC_FF0561 Peaks t acid in acetonitrile Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC © 2018 Restek...

Fast, Low-Solvent Analysis of Cannabinoids Increases Lab Productivity and Decreases Solvent Costs (PDF 2595kB)

.../Vis @ 228 nm; Instrument: UHPLC Peaks tR (min) 1. Cannabidivarinic acid (CBDVA) 1.570 2. Cannabidivarin .6 mm cat.# Raptor ARC-18 EXP Guard Column Cartridge UHPLC 3-pk. 9314U0252 9314U0253 Raptor ARC-18...

Raptor ARC-18 LC Columns Brochure (PDF 4942kB)

... then evolved to bring that same improved speed, efficiency, and selectivity to UHPLC analyses by offering 1 to bioanalytical work, whether you use traditional HPLC or UHPLC instruments, we’re all looking to simplify setup...

PFAS LC Column Anatomy: Which Phase, Dimensions, and Particle Type Are Best? (PDF 1483kB)

... Instrument UHPLC Notes A PFAS delay column (cat.# 27854) was installed between the pump mixer %B 0.00 0.5 15 85 5.00 0.5 15 85 Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC www...

Raptor HILIC-Si: Simplify the Switch to HILIC (PDF 2013kB)

... for increasing sensitivity and selectivity in LC-MS analyses. • Fully compatible with both HPLC and UHPLC : 600 bar/8,700 psi Properties: • Compatible with both HPLC and UHPLC instruments. • Restek’s 2.7 μm...

Restek LC Columns and Accessories (PDF 17409kB)

... Overview: Premium, long-lasting, and reproducible columns for HPLC and UHPLC; fully scalable from 1.8 μm to 3 μm to 5 μm. Ideal for: Scaling methods between HPLC and UHPLC. Instruments supported: • Agilent...

One Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics (PDF 903kB)

... Detector MS/MS Ion Mode: ESI+/ESIMode: Scheduled MRM Instrument UHPLC Notes 1. Positive and negative B: 0.1% Formic acid in acetonitrile Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC...

Thomson SINGLE StEP Filter Vials (PDF 738kB)

... autosamplers, such as Agilent, Shimadzu, and Waters—even UHPLC instrumentation. • Easy-to-use vials offer fast UHPLC instruments. Figure 1: The patented design of Thomson SINGLE StEP filter vials allows you...

Analysis of d- and l-Amphetamine and Methamphetamine Enantiomers for High-Throughput Labs (PDF 568kB)

... Guard Column Cartridge UHPLC 3-pk. 9304U0252 9304U0253 Raptor C18 EXP Guard Column Cartridge 2.7 μm 3 930450253 930450250 Maximum cartridge pressure: 1,034 bar/15,000 psi* (UHPLC), 600 bar/8,700 psi (2.7 μm...

Can Nonmatching Mycotoxin SIDA Internal Standards Be Used for Stable Isotope Dilution Assay Calibration? (PDF 452kB)

....0 mm cat.# 5 x 4.6 mm cat.# Raptor Biphenyl EXP Guard Column Cartridge UHPLC 3-pk. 9309U0252 cartridge pressure: 1,034 bar/15,000 psi* (UHPLC), 600 bar/8,700 psi (2.7 μm); 400 bar/5,800 psi (5 μm...

Diagnosing and Preventing High Back Pressure Problems in LC Systems (PDF 5850kB)

...’s a fact of life for HPLC and UHPLC analyses. The force that is necessary to push a liquid mobile phase dead volume. • Fit most standard 12 x 32 mm autosamplers, including UHPLC instruments. www...

Successful Strategies for the Analysis of EtG and EtS in Urine (PDF 1274kB)

...: UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809) Temp.: 35 °C Sample Diluent: 0 Shield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809) Temp.: 35 °C Sample Conc.: 50 μL of urine...

OPTI-MAX Check Valves Instruction Sheet (PDF 295kB)

... parts and supplies, visit www.restek.com OPTI-MAX Check Valves INNOVATIVE The UHPLC Connection1...

High-Throughput Analysis of Mycotoxins in Cannabis CBD Oil Pairs Simplified Cleanup with LC-MS/MS Sensitivity (PDF 1230kB)

....00 min (65%); Flow: 0.7 mL/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: 0 .# 5 x 4.6 mm cat.# Raptor Biphenyl EXP Guard Column Cartridge UHPLC 3-pk. 9309U0252 9309U0253...

5.5 Minute LC-MS/MS Analysis of Mycotoxins in Peanut Powder (PDF 945kB)

...%); Flow: 0.5 mL/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: Weighed 1 Cartridge UHPLC 3-pk. 9309U0252 9309U0253 Raptor Biphenyl EXP Guard Column Cartridge 2.7 μm 3-pk. 9309A0252...

Improved Screening Method for Acylcarnitines and Amino Acids in Dried Blood Spots by LC-MS/MS (PDF 1338kB)

... and amino acids was performed on a Shimadzu Nexera UHPLC equipped with a SCIEX API 4500 MS/MS. Mass Source: Electrospray Ion Mode: ESI+ Instrument UHPLC Notes 50 μL of whole blood was spotted...

News: New UHPLC Guard Cartridges Deliver Ultimate Protection for Restek’s 1.8 μm Raptor Columns

Raptor 1.8 μm LC columns unleashed superior Raptor performance on UHPLC analyses, and now Restek has released EXP UHPLC guard column cartridges made specifically for Raptor 1.8 μm columns. These new guards provide extreme protection from particulates and matrix contamination, … Continue reading

Restek Essentials: Featured Chromatography Products 2018.1 (PDF 5439kB)

... autosamplers, including UHPLC instruments. Samples available! Contact your local Restek representative Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing WARNING: Do not use EXP ferrules...

Restek Essentials: Featured Chromatography Products 2021.1 (PDF 4411kB)

.... 9309515 150 mm ea. 9309565 250 mm ea. 9309575 Superficially porous particles that boost UHPLC HPLC to UHPLC. Si CH3 CH3 O Learn more about the time-tested Biphenyl phase at www...

A Fast Dilute-And-Shoot Method for Simultaneous 5-Hydroxyindoleacetic Acid (5-HIAA), Vanillylmandelic Acid (VMA), and Homovanillic Acid (HVA) LC-MS/MS Analysis in Human Urine (PDF 631kB)

...: Methanol Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC Notes The synthetic urine (Surine : ESIMode: MRM Instrument UHPLC Notes The human urine sample is the Bio-Rad Lyphochek quantitative urine...

Analyse LC-MS/MS de mycotoxines dans la poudre de cacahuète en moins de 6 minutes (PDF 976kB)

...: MRM; Instrument: UHPLC; Notes: Weighed 1.00 gram of peanut powder in a 50 mL centrifuge tube Qté 5 x 2,1 mm Réf. 5 x 3.0 mm Réf. 5 x 4.6 mm Réf. Cartouches Raptor Biphenyl EXP UHPLC Lot de...

Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV (PDF 686kB)

... by HPLC provides fast results without the expense of UHPLC equipment. • Baseline resolution improves and accurately without investing in UHPLC equipment. LC_FF0529 Peaks 1. Benzo[c]fluorene 2. Cyclopenta...

Fast, Accurate LC-MS/MS Method for Folate Deficiency Biomarkers in Plasma (PDF 513kB)

... Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: Sample Preparation Method: 1. Aliquot 380 μ /Ferrule) 10-pk. 25938 EXP Hand-Tight Nut (w/o Ferrule) ea. 25939 EXP Reusable Fittings for HPLC & UHPLC...

Effect of Organic Solvent on Selectivity in LC Separations (PDF 1081kB)

... techniques. Description Particle Size Size qty. cat.# Raptor C18 EXP Guard Column Cartridge UHPLC 5 x 2 .1 mm 3-pk. 9304U0252 UHPLC 5 x 3.0 mm 3-pk. 9304U0253 2.7 μm 5 x 2.1 mm 3-pk. 9304A0252 2.7 μm 5 x 3...

News: Restek Unleashes Superior Raptor Performance on Your UHPLC Analyses — Now with 1.8 μm Particles

Raptor LC columns have been providing higher efficiencies, faster analyses, and rock-solid reliability to HPLC users for several years. And they have just evolved again. Restek has brought the benefits of Raptor columns to those who have made the leap … Continue reading

LC-MS/MS Analysis of 58 Antipsychotics and Antidepressants in Human Urine (PDF 792kB)

... Instrument UHPLC Notes Drug-free human urine (BioIVT) was fortified with 58 analytes at 2500 ng 9309A0253 9309A0250 Maximum cartridge pressure: 1,034 bar/15,000 psi* (UHPLC), 600 bar/8,700 psi (2...

www.restek.com Quantitative LC-UV Method for CBD in Topicals... (PDF 897kB)

....7 μm 5 x 4.6 mm 3-pk. 9314A0250 Maximum cartridge pressure: 1,034 bar/15,000 psi* (UHPLC), 600 bar/8 ,700 psi (2.7 μm); 400 bar/5,800 psi (5 μm) * For maximum lifetime, recommended maximum pressure for UHPLC...

Featured Application: Polycyclic Aromatic Hydrocarbons (PAHs) on a Pinnacle II PAH HPLC Column Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV

... ~15-minute analysis of PAHs by HPLC provides fast results without the expense of UHPLC equipment with a Pinnacle II PAH column allows labs to report PAH results quickly and accurately without investing in UHPLC ... minute analysis without the expense of UHPLC equipment....

Highly Selective LC-MS/MS Analysis of Mycotoxins from Multiple Classes (PDF 556kB)

...; Detector: MS/MS ; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC Length 2.1 mm cat.# 3.0 mm cat.# 4 & UHPLC for 10-32 fittings and 1/16" tubing EXP Hand-Tight Fittings Unidirectional Raptor EXP Guard...

Rapid, Simple 4.5-Minute Pyrrolizidine Alkaloids Analysis by LC-MS/MS (PDF 1056kB)

...L/min; Detector: MS/MS; Ion Mode: ESI+; Instrument: UHPLC. Length 2.1 mm cat.# 3.0 mm cat.# 4.6 mm cat.# 1 that although a UHPLC instrument was used in this example, it is not necessary for this analysis. Excellent peak...

Raptor SPP Core-Shell LC Columns

Raptor SPP LC columns offer higher efficiency for drastically faster analysis times, better selectivity for substantially improved resolution, increa...

News: The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses

Published By: Restek Corporation Year of Publication: 2017 Link: www.restek.com/Technical-Resources/Technical-Library/General-Interest/gen_GNAR2803-UNV Abstract: Superficially porous particles (commonly referred to as SPP or “core-shell” particles) have been proven to provide fast and efficient LC separations. These particles feature a solid, impermeable core enveloped by a … Continue reading

Influencing Selectivity in LC-MS Peptides Analysis on the Raptor ARC-18 Column (PDF 989kB)

... Instrument UHPLC 3 www.restek.com Figure 3: Effects of Acid Concentration on Retention and Selectivity. LC Detector MS Ion Mode: ESI+ Mode: MS scan Instrument UHPLC 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4...

Overcoming the Effects of Highly Organic Protein Precipitation Extracts on LC Peak Shape Using Direct Injection (PDF 585kB)

... and undiluted extracts) were analyzed using a Shimadzu Nexera UHPLC equipped with a SCIEX API 4500 MS/MS under bar Detector MS/MS Ion Mode: ESI+ Mode: Scheduled MRM Instrument UHPLC Notes Sample Preparation...

More of What You Need to Keep Your LC Flowing: Fittings, tubing, mobile phase management & safety, and more. (PDF 3764kB)

.... 27713 PEEK-HT Hex-Head Fittings for HPLC and UHPLC • One-piece design; no ferrule required. • Use ,000 psi JR-T-6003-M10 ea. 27756 4 www.restek.com Stainless Steel Unions, Tees, and Crosses for UHPLC...

Tech Tip: Column Conditioning Ensures Consistent EtG/EtS Results (PDF 402kB)

... Size: 2.7 μm Pore Size: 90 Å Guard Column: UltraShield UHPLC precolumn filter, 0.2 μm frit (cat...

Restek Essentials: Featured Chromatography Products 2019.1 (PDF 4834kB)

... restek.com/LCguard Sub-2 μm Columns for Lightning-Fast UHPLC Accelerate Your GC Analyses and Increase Superficially porous particles that boost UHPLC efficiencies further for high-throughput LC-MS/MS. Fully...

Restek Essentials: Featured Chromatography Products 2019.1 (PDF 4834kB)

... restek.com/LCguard Sub-2 μm Columns for Lightning-Fast UHPLC Accelerate Your GC Analyses and Increase Superficially porous particles that boost UHPLC efficiencies further for high-throughput LC-MS/MS. Fully...

Raptor Polar X: Separate a Wide Variety of Polar Analytes with a Novel Hybrid Stationary Phase (PDF 2246kB)

... Mode: ESI-; Mode: MRM; Instrument: UHPLC. Time (min) 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5 /MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: A 200 μL aliquot of protein hydrolysate formula...

7.5-Min Screening Analysis of Multiresidue Pesticides in Brown Rice Flour (PDF 1504kB)

... and modern UHPLC instruments. Analysis of multiresidue pesticides is no easy task, and it’s made even more .4 95 5 Detector MS/MS Ion Mode: ESI+/ESIMode: Scheduled MRM Instrument UHPLC Notes Although...

Fast and Rugged Direct Analysis of Polar Pesticides in Spinach (PDF 1172kB)

...; Instrument: UHPLC; Notes: Frozen spinach was added to a Blixer processor with dry ice (3:1-4:1 ratio times. • 2.7 μm Raptor core-shell particles provide UHPLC-like speed and efficiency on all makes...

Fast, Direct Analysis of Underivatized Amino Acids in Infant Formula (PDF 921kB)

... Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: A 200 μL aliquot of protein hydrolysate formula equilibration times. • 2.7 μm Raptor core-shell particles provide UHPLC-like speed and efficiency on all makes...

Resprep PPT 96-Well Plates Sales Sheet (PDF 1620kB)

....1% formic acid Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Extraction Procedure: 1...

Method Development and Column Selection: How the FluoroPhenyl Phase Provides the Power of HILIC and Reversed-Phase Modes in One Column (PDF 2146kB)

...L/min) 0.00 08 20 0.8 Max Pressure: 400 bar Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC .00 0.8 95 5 Max Pressure: 400 bar Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC...

Analysis of Synthetic Cannabinoids and Metabolites: Adding New Compounds to an Existing LC-MS/MS Method (PDF 870kB)

... performed on a Waters ACQUITY UPLC I-Class equipped with a Xevo TQ-S and a Shimadzu Nexera UHPLC equipped + Mode: MRM Instrument UHPLC Precursor Quantifer Qualifier Peaks tR (min) Ion Product Ion Product Ion...

LC-MS/MS Analysis of Paraquat and Diquat without Ion-Pairing Reagents (PDF 530kB)

... & UHPLC for 10-32 fittings and 1/16" tubing EXP Hand-Tight Fittings Raptor EXP Guard Cartridges...

Trace-Level Analysis of Melamine and Related Compounds by LC-MS/MS (PDF 763kB)

.... EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing EXP Hand-Tight Fittings...

LC-MS/MS Analysis of Urinary Free Cortisol and Cortisone without Matrix Interferences (PDF 515kB)

... Pressure: 400 bar Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes: Sample Preparation...

Simplified Methylmalonic Acid Analysis without Derivatization in Human Plasma by LC-MS/MS (PDF 584kB)

...,700 psi. Apply Force LC columns to all of your HPLC and UHPLC instrument platforms at www...

Fast, Sensitive LC-MS/MS Analysis of GHB and Related Compounds in Human Blood for Forensic Testing (PDF 630kB)

... LC columns to all of your HPLC and UHPLC instrument platforms at www.restek.com/force Hybrid Ferrule...

Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases (PDF 1244kB)

.../MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes The urine from a non-tobacco user was fortified Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Samples were processed with a liquid...

Method Evaluation: Analysis of Phosphatidylethanol (PEth) in Human Whole Blood by LC-MS/MS (PDF 874kB)

... Mode: ESIMode: MRM Instrument UHPLC Notes Sample Preparation Procedure (Provided by Redhot .51 0.5 30 70 3.50 0.5 30 70 Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC Notes Sample...

Eliminate the Impact of Instrument-Related PFAS Interferences by Using a Delay Column (PDF 761kB)

... Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC LC_EV0549 3 www.restek.com Adding a delay : MRM Instrument UHPLC Notes A PFAS delay column (cat.# 27854) was installed before the injector...

Eliminate the Impact of Instrument-Related PFAS Interferences by Using a Delay Column (PDF 761kB)

... Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC LC_EV0549 3 www.restek.com Adding a delay : MRM Instrument UHPLC Notes A PFAS delay column (cat.# 27854) was installed before the injector...

Simple Large Volume Injection Method for Trace-Level Glyphosate in Bottled Drinking Water (PDF 754kB)

... UHPLC 2 www.restek.com Featured Products Reference Standards Analytical Column Maintenance...

Rapid Analysis of C3 Epimers of 25-Hydroxyvitamin D in Serum by LC-MS/MS (PDF 559kB)

....01 0.6 25 75 7.00 0.6 25 75 Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Sample UHPLC Notes Serum (400 μL) was mixed with 0.2 M ZnSO₄ (400 μL) in a 4 mL glass vial. Then, 800 μ...

Integrating the Analysis of Ultrashort-Chain PFAS: Method Development for Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy PFAS (PDF 1070kB)

... 11.0 0.25 80 20 Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC Notes A PFAS delay column profitability, you need columns to last longer, data to be reproducible, and existing HPLC and UHPLC...

Restek Essentials (PDF 1623kB)

... sub-2 μm per analisi UHPLC super veloci Accelera le tue analisi GC e aumenta la produttività del tuo porose aumentano ulteriormente l'efficienza del tuo UHPLC per un rendimento LC-MS/MS più alto...

Restek LC Guard Column Selector: How to Find the Right Guard Fast

...’re performing UHPLC, you are using a smaller particle column that generates high back pressure, so you need with all HPLC through UHPLC instruments and cartridges (rated to 20,000 psi [1400+ bar...

“The Big Pain”: Development of Pain-Free Methods for Analyzing 231 Multiclass Drugs and Metabolites by LC-MS/MS (PDF 4733kB)

...: Scheduled MRM Instrument UHPLC Notes 1. Positive and negative polarity data were collected...

Comprehensive LC-MS/MS Analysis of 15 Bisphenols in 8 Minutes (PDF 519kB)

...: Methanol Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC LC_FS0518 www.restek.com 3 Length 2...

Novel Stationary Phase for Comprehensive PFAS Analysis: Ultrashort-Chain (C2, C3), Alternative, and Legacy Compounds (PDF 656kB)

...; Instrument: UHPLC. LC_EV0569_LC_EV0570 Questions? Contact us or your local Restek representative (www...

Improve Analysis of Aldehydes and Ketones in Air Samples with Faster, More Accurate Methodology (PDF 417kB)

...), Flow: 0.8 mL/min; Detector: UV/ Vis @ 365, 4.8 nm; Instrument: UHPLC; Notes: *Mobile phase B...

Rapid Perfluorinated Alkyl Acid Analysis by LC-MS/MS Increases Sample Throughput (PDF 640kB)

.../MS Ion Mode: ESIMode: MRM Instrument UHPLC LC_EV0536 tR Conc. Precursor Product Peaks (min) (ng...

Trace-Level Analysis of Metanephrines in Plasma by HILIC LC-MS/MS (PDF 712kB)

....00 0.3 10 90 5.00 0.3 10 90 Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Solid 5.00 0.3 10 90 Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Solid Phase...

High-Throughput Analysis of Immunosuppressive Drugs from Whole Blood by LC-MS/MS (PDF 524kB)

... Max Pressure: 305 bar Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Sample + Mode: MRM Instrument UHPLC Notes Sample Preparation Procedure: Human whole blood (100 μL) was mixed...

Featured Application: Fast Analysis of Bisphenol A on a Raptor Biphenyl LC Column 4-Minute Bisphenol A (BPA) Analysis Increases Sample Throughput

...—compatible with both HPLC and UHPLC systems. Bisphenol A (BPA) is a high . In addition, Raptor Biphenyl columns can be used with both conventional HPLC and UHPLC instruments. For BPA...

ChromaBLOGraphy: Analyze PAHs by HPLC without Acetonitrile

Labs that are trying to cope with the acetonitrile shortage are trying to reduce solvent usage, or going to an alternate mobile phase. One way to reduce solvent usage is to minimize your column volume. This means going to a narrower column and possibly a shorter column as well. Ultra high pressure liquid chromatography (UHPLC) [...]

ChromaBLOGraphy: Live Long and Prosper-How to make your HPLC columns last longer

We frequently get questions about how long an HPLC or UHPLC column should last. To borrow a phrase from my colleagues, “Your mileage may vary.”  Columns are like cars, so their lifetime depends how they are used and how well they are maintained. That being said, some phases are known to be more rugged than [...]

News: Apply Force to Your LC Methods with Restek’s New High-Performance Columns

Restek’s new Force performance LC columns give you the power to maximize instrument uptime, increase productivity, and transfer methods across your entire lab,  from your trusted-but-aged HPLC to the cutting-edge UHPLC you just plumbed last week. This incredibly rugged and … Continue reading

ChromaBLOGraphy: Choosing a guard cartridge holder for LC

If you need help choosing one of the Trident guard cartridge holders, check out our information found in the HPLC and UHPLC FAQs section of our website. The FAQs contain a lot of good information and are often overlooked. For most of our columns we prefer the Trident Direct Guard holder system, because of its […]

PFAS LC Column Anatomy: Which Phase, Dimensions, and Particle Type Are Best?

... the associated ultra-high pressure, which allows labs using either UHPLC or HPLC instruments to obtain fast without the capital expense of a UHPLC instrument. For those labs already using UHPLC...

ChromaBLOGraphy: Mass Overload on Superficially Porous Particles

The benefits of superficially porous particles are without question.  Who wouldn’t want to perform faster separations without the need for expensive UHPLC instrumentation?  It sounds too good to be true.  There must be some drawback – right? The solid, impermeable core present in Raptor™ particles increases column efficiency by decreasing the diffusion path at the […]

ChromaBLOGraphy: What can I use to filter my mobile phase?

If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed […]

Analysis of Fentanyl and Its Analogues in Human Urine by LC-MS/MS (PDF 537kB)

....40 238.19 111.06 Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Human urine...

Simultaneous Analysis of Catecholamines and Metanephrines in Urine by LC-MS/MS (PDF 1289kB)

...: ESI+ Mode: MRM Instrument UHPLC Notes Solid Phase Extraction Procedure: A 200 μL aliquot of urine...

Evaluation of a Simple Method for Methylmalonic Acid Analysis in Human Plasma by LC-MS/MS (PDF 735kB)

...L). • Fit most standard 12 x 32 mm autosamplers, including UHPLC instruments. Conclusion...

Growing Analytical Solutions for Cannabis Testing: Innovative Products and Expertise for Fast, Accurate, Reliable Results (PDF 8494kB)

... performance so you won’t need to buy expensive UHPLC equipment or extend your capital investment when analysis times for cannabinoids without the expense of UHPLC equipment. Applications PRODUCT POTENCY...

Restek Essentials: Featured Chromatography Products 2020.3 (PDF 4005kB)

... autosamplers, including UHPLC instruments. Also available in eXtreme, low-evaporation, and nano versions...

News: Hydrogen as Carrier Gas: Always Available, Cost Effective and Fast – A Personal Reflection

Author: Jaap de Zeeuw Restek Corporation Published By: Chromatography Today Issue: November / December 2012 Year of Publication: 2012 Link: http://www.chromatographytoday.com/articles/hplc-uhplc-lc-ms/31/jaap_de_zeeuw/hydrogen_as_carrier_gas_always_available_cost_effective_and_fast_a_personal_reflection/1333/ Abstract: Hydrogen is on the agenda again. Since the supply of helium has become a challenge, many labs are … Continue reading

News: Keep Your LC Running Smoothly with New Accessories from Restek

Simplify maintenance with the latest LC accessories from Restek. Reusable EXP2 HPLC/UHPLC fittings allow repeated 20,000+ psi seals to be easily made without wrenches. The patented titanium/PEEK ferrule provides zero-dead-volume connections without compromising high-pressure seals. Their compact design makes them … Continue reading

ChromaBLOGraphy: Which LC column should I use for my method?

We understand that with so many products on the market, choosing a column to get started can be difficult. Of course, column selection depends on what type of method you are following and what kind of LC system you have in your lab. Let’s begin by looking at our choices for the more traditional, fully […]

ChromaBLOGraphy: LC Preventative Maintenance Tips and Tricks – The UltraShield

We’re providing a creative trick for extending guard cartridges and column lifetimes.  This preventative maintenance consumable also acts as a handy troubleshooting tool for problematic back pressure readings.  Drum roll….The UltraShield! https://www.restek.com/pdfs/730-70-003.pdf Normally, the UltraShield is used as an affordable UHPLC pre-column filter and takes the place of a guard column.  These filters have minimal […]

ChromaBLOGraphy: EXP Fittings: Which Nut Do I Need?

Our brilliant friends at Optimize Technologies are continually innovating and making our lab lives easier. We love their patented EXP Titanium Hybrid Ferrule technology that is truly universal. You can use it on any tubing, any 10-32-port, any instrument—wrench-tight to 20,000 psi. This fully UHPLC-capable EXP ferrule is used with all EXP nuts. Restek offers […]

ChromaBLOGraphy: Which LC Phase Do I Need?

So you’re thinking about doing HPLC (or UHPLC) and don’t have a method? You’ve never even seen a method for anything remotely similar. You might have even searched our HPLC chromatogram database for the application and haven’t found anything.  (After all, that’s the quickest and easiest way to get some ideas.) What to do… Let’s [...]

Big Pain Assays Aren't a Big Pain with the Raptor Biphenyl LC Column (PDF 2205kB)

...: ESI+/ESI-; Mode: Scheduled MRM; Instrument: UHPLC; Notes: 1. Positive and negative polarity data were...

Analysis of Pesticides and Mycotoxins in Cannabis Brownies (PDF 2464kB)

... Detector MS/MS Ion Mode: ESI+/ESIMode: MRM Instrument UHPLC Notes Brownies were pulverized using a SPEX...

News: Eliminate the Impact of PFAS Instrument Contamination with a Restek PFAS Delay Column

Per-and polyfluorinated alkyl substances (PFAS) are often used in tubing and other liquid-contacting parts of an HPLC system, where they can leach into the mobile phase and interfere with sample analysis. This contamination is particularly problematic when analyzing at trace … Continue reading

ChromaBLOGraphy: What is the pressure limit for my LC column?

This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ROC LC columns, the maximum operating pressure is 400 […]

ChromaBLOGraphy: Choosing a guard cartridge for LC

So you have a guard cartridge holder of some sort, and you don’t have the guard cartridge to put inside. If you happen to have missed that last blog post about the holder, click on the following link. Choosing a guard cartridge holder for LC Let’s determine what type of guard cartridge you need. A […]

ChromaBLOGraphy: What is SPP and when should I use a Raptor column?

While this sounds like a very broad question, I’m sure it is crossing some of your minds. To start out, SPP stands for superficially porous particles. It has also been called “core-shell”.   If you are a veteran in the world of HPLC, this term is old hat for you. If you are new to the […]

ChromaBLOGraphy: How to decrease run time for reversed phase LC

There are several ways to accomplish this, although it boils down to roughly 4 possibilities. Use a shorter column length. This may be appropriate if you have plenty of retention for your analyte(s), if you only have one or a few analytes AND if you do not have interference peaks in your chromatogram. It does, […]

A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS (PDF 1398kB)

...: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: Sample Preparation Procedure: A 500 μ...

News: New Raptor™ FluoroPhenyl Phase: the Power of HILIC and RP Modes in One LC Column

Restek has now extended the speed and reliability of Raptor™ column technology into the HILIC realm with the addition of Raptor™ FluoroPhenyl columns. Restek’s Raptor™ FluoroPhenyl phase offers chromatographers the ability to run in reversed-phase or HILIC mode for a … Continue reading

News: Simplify the Switch to HILIC with Raptor HILIC-Si Columns

Restek is bringing the speed and reliability of Raptor LC columns into the HILIC realm with the addition of the Raptor HILIC-Si column. HILIC is an increasingly popular alternative to reversed-phase LC for challenging polar analytes because it provides better … Continue reading

News: Liquid Chromatography’s Complementary Role to Gas Chromatography in Cannabis Testing

Authors: Justin Steimling, Ty Kahler Restek Corporation Published By: Supplement to LCGC North America Year of Publication:  June 2018 Volume, Number: Volume 36, Number s6 Link: http://files.pharmtech.com/alfresco_images/pharma/2018/06/13/f119cb54-ce43-4628-95f8-af1aa07bb6ff/LCGC_NAmerica_June2018Supp.pdf Abstract: The absence of consensus methods for cannabis testing is a challenging, but refreshing opportunity for … Continue reading

Analytical Method for Polycyclic Aromatic Hydrocarbons (PAHs) in Yerba Mate Tea Using Modified QuEChERS, Solid Phase Extraction and GC-TOFMS and GC-MS/MS (PDF 634kB)

..., Determination of amantadine and rimantadine in chicken muscle by QuEChERS pretreatment method and UHPLC coupled...

LC Method Translation: Will Switching Column Dimensions Speed Up Sample Analyses? Answer that question in seconds with Restek’s online Pro EZLC method translator.

...-of-the-art UHPLC instrument to a production facility with traditional HPLC instruments, a method translator can...

The Restek Biphenyl: Leading Resolution in LC since 2005

With ten years of unrivaled performance, Restek's time-tested Biphenyl has been proven to be the ideal LC column for clinical diagnostic, pain, pharm...

Direct Analysis of Morphine, M3G and M6G Metabolites, and Related Compounds in Urine by LC-MS/MS

... UHPLC equipped with a SCIEX API 4500 MS/MS. Detection was performed using electrospray ionization with a Restek Force Biphenyl 1.8 μm, 50 mm x 2.1 mm column equipped with an UltraShield UHPLC precolumn filter...

Restek Liquid Chromatography (LC) Columns — Cleaning Recommendations

... to the pump eluent. For UHPLC columns (1.9 µm), DO NOT FLIP THE COLUMN. Instead, flush...

Featured Application: Pyrrolizidine Alkaloids on Raptor ARC-18 Rapid, Simple 4.5-Minute Pyrrolizidine Alkaloids Analysis by LC-MS/MS

... LaN in just 3.2 minutes (4.5 minute total run time). Note that although a UHPLC instrument was used...

ChromaBLOGraphy: Eastern Canada Trace Organic Workshop

Last month, I attended two trace environmental workshops at the National Water Research Institute (CCIW) in Burlington, Ontario; the Workshop on LC-MS/MS Applications in Environmental Analysis and Food Safety and the Eastern Canada Trace Organic Workshop (ECTOW). The full program can be found here. This was my first exposure to work done on Thermo’s Orbitrap, which [...]

ChromaBLOGraphy: Do you love your pet? Then you will love our Raptor™ Biphenyl LC column.

Have you ever faced this? Your pet has a stomach problem and nothing helps except to visit the veterinarian? Most likely, your beloved dog or cat is infected with parasitic protozoans, causing a disease called coccidiosis. Help is in sight; the veterinarian will use a pharmaceutical out of the group of coccidiostats and there is […]

ChromaBLOGraphy: Top 16 for 2016 – Restek LC Article Makes the Cut

If you are an avid chromatographer you likely subscribe to LC/GC magazine or its popular electronic version, E-Separation Solutions. It is definitely the place to go for news and information related to all things Chromatography. Over the years Restek has had many an article published in LC/GC and, full disclosure, we advertise there as well. Who […]

News: New Raptor™ ARC-18 Columns Are Born for LC-MS/MS

The birth of Restek’s new Raptor™ SPP LC column line began with the innovative Biphenyl phase, and it has now grown to include a new Restek® phase: the ARC-18. Designed and intended specifically for use on LC-MS/MS systems, the Raptor™ … Continue reading

News: Raptor™ Speed, Efficiency, and Ruggedness is Now Available in C18

Every LC lab has a cache of C18s, but every C18 is not created equal. Because the chemistry tends to be similar, the silica support that carries this ubiquitous octadecylsilane phase becomes vitally important. When you need a general-purpose LC … Continue reading

News: Struggling with EtG/EtS matrix interferences?

A Raptor EtG/EtS column is your solution! Typical methods for EtG and EtS analysis have several limitations: poor resolution from matrix components, long run times that limit sample throughput, and short column lifetimes. Restek has developed a new Raptor EtG/EtS … Continue reading

News: Separate a Wide Variety of Polar Analytes with New Raptor Polar X Columns

Analyzing polar compounds using liquid chromatography has historically been a challenge. Poor retention and peak shape, complex mobile phases that may not be MS-friendly, long equilibration times, low sensitivity, and sample derivatization are all complications that reduce lab efficiency and … Continue reading

News: Automated Inline Pigment Removal for the Analysis of Pesticide Residues in Spinach by Liquid Chromatography Tandem Mass Spectrometry

Author(s): Sharon A. Lupo, Randall L. Romesberg, Xiaoning Lu Restek Corporation Published By: Journal of Chromatography A Year of Publication: 2020 Link: https://www.sciencedirect.com/science/article/pii/S0021967320307470 Abstract: An automated inline sample preparation (ILSP) method has been developed for pesticide residue analysis in spinach … Continue reading

Growing Analytical Solutions For Cannabis Testing Innovative Products And Expertise For Fast, Accurate, Reliable Results

...-minute analysis that is compatible with any HPLC instrument—so you get UHPLC speed on your existing equipment analysis times for cannabinoids without the expense of UHPLC equipment...

Rapid Analysis of 17 Bile Acids in Human Plasmaby LC-MS/MS

... minimum resolution of isomers. In this study, a simple UHPLC method was developed for the simultaneous LC-MS/MS analysis of bile acids in human plasma was performed on a Shimadzu Nexera UHPLC paired...

News: Experience Selectivity Accelerated With New Raptor™ SPP LC Columns and Guards

Restek is excited to announce the evolution of superficially porous particles with the introduction of Raptor™ LC columns. Superficially porous particles (commonly referred to as SPP or “core-shell” particles) changed the world of LC by dramatically boosting column efficiency and … Continue reading

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Cannabinoids in Gummy Edibles on Raptor ARC-18

LC_GN0667

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Parabens on Raptor C18

LC_PH0531

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