share:

HPLC & UHPLC Columns > HPLC & UHPLC Analytical Columns

Browse our complete line of HPLC & UHPLC Analytical Columns

Chromatography Columns > HPLC & UHPLC Columns

Browse our complete line of HPLC & UHPLC Columns

Replacement Filters for UltraLine UHPLC In-Line Filter

...Replacement Filters for UltraLine UHPLC In-Line Filter ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Pinnacle DB PAH UHPLC Columns

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Pinnacle DB PAH UHPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB IBD UHPLC Columns (USP L68)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Pinnacle DB IBD UHPLC Columns ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Stainless Steel Fittings for UHPLC

...Stainless Steel Fittings for UHPLC ... Ideal for connecting stainless steel tubing in UHPLC systems. For 1...

PFAS Delay Column

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

USLC Method Development Toolbox

All the Right ToolsAll in One Toolbox

... four USLC stationary phases in one convenient package. Available for UHPLC (1.9 μm) and HPLC ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... four USLC stationary phases in one convenient package. Available for UHPLC (1.9 μm) and HPLC...

PEEK-HT Hex-Head Fittings for HPLC and UHPLC

...PEEK-HT Hex-Head Fittings for HPLC and UHPLC...

UltraLine UHPLC In-Line Filter

...-effective protection for UHPLC systems. Reliable way to extend column lifetime. Leaktight to 15 ... UltraLine UHPLC In-Line Filter ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... -effective protection for UHPLC systems. Reliable way to extend column lifetime. Leaktight to 15...

UltraShield UHPLC PreColumn Filter

... Cost-effective protection for UHPLC systems. Reliable way to extend column ... UltraShield UHPLC PreColumn Filter ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... Cost-effective protection for UHPLC systems. Reliable way to extend column...

Stainless Steel Unions, Tees, and Crosses for UHPLC

...Stainless Steel Unions, Tees, and Crosses for UHPLC ... Ideal for quickly and reliably connecting stainless steel tubing in UHPLC systems...

Ultra Quat Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Replacement Trident PEEK Ferrules

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Pinnacle DB Silica Columns (USP L3)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB PFP Propyl Columns (USP L43)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB Biphenyl Columns (USP L11)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB Cyano Columns (USP L10)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB C8 Columns (USP L7)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB C18 Columns (USP L1)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle DB Aqueous C18 Columns (USP L1)

.... Because performance begins with the support, our Pinnacle DB UHPLC columns start with base -deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Force FluoroPhenyl LC Columns (USP L43)

...-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure and 1.8 µm UHPLC, so you can easily transfer and optimize methods without extensive calculations. Apply ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Force Biphenyl LC Columns (USP L11)

...-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure and 1.8 µm UHPLC, so you can easily transfer and optimize methods without extensive calculations. Apply ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

PEEK Tips for Trident Direct Guard Cartridge Systems

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Roc LC Guard Column Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Replacement PEEK Ferrules for Roc LC Guard Column Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Pinnacle II PAH Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Viva Silica Columns (USP L3)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Allure AK Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Allure Silica Column for MOSH/MOAH Analysis

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Silica Columns (USP L3)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C4 Columns (USP L26)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C1 Columns (USP L13)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Allure Organic Acids Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Pinnacle II PAH HPLC Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Carbamate Columns

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Allure Silica Columns (USP L3)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Amino Columns (USP L8)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

EXP2 Reusable Fittings for HPLC & UHPLC

for 10-32 fittings and 1/16" tubing

...-swaged ferrules mean vendor-specific tubing is not required. Suitable for both HPLC and UHPLC UHPLC connections and/or non-ZDV connections.Hybrid Ferrule U.S. Patent No. 8201854, EXP ... EXP2 Reusable Fittings for HPLC & UHPLC ... : EXP and EXP2 Fittings: Reusable fittings for easy, reliable HPLC and UHPLC connections. ... -swaged ferrules mean vendor-specific tubing is not required. Suitable for both HPLC and UHPLC...

Raptor HILIC-Si LC Columns (USP L3)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns at www . Fully compatible with both HPLC and UHPLC when using low backpressure HILIC mobile phases ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns ... . Fully compatible with both HPLC and UHPLC when using low backpressure HILIC mobile phases...

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Titanium Hybrid Replacement Ferrules

... for reliable UHPLC use up to 20,000+ psi! Patented ferrule can be installed repeatedly without ,000+ psi (1,400+ bar) UHPLC connection with either fitting style, simply wrench-tighten an extra 1/4 to 1 ... EXP Reusable Fittings for HPLC & UHPLC ... Fittings: Reusable fittings for easy, reliable HPLC and UHPLC connections. ... for reliable UHPLC use up to 20,000+ psi! Patented ferrule can be installed repeatedly without...

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Hand-Tight Coupler

... for reliable UHPLC use up to 20,000+ psi! Patented ferrule can be installed repeatedly without ,000+ psi (1,400+ bar) UHPLC connection with either fitting style, simply wrench-tighten an extra 1/4 to 1 ... EXP Reusable Fittings for HPLC & UHPLC ... Fittings: Reusable fittings for easy, reliable HPLC and UHPLC connections. ... for reliable UHPLC use up to 20,000+ psi! Patented ferrule can be installed repeatedly without...

Force C18 LC Columns (USP L1)

...-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure and 1.8 µm UHPLC, so you can easily transfer and optimize methods without extensive calculations. Apply ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Hex-Head Fittings

...+ bar)—no tools needed! For a reliable 20,000+ psi (1,400+ bar) UHPLC nuts. Failure to use EXP fittings according to the included instructions may result in unsafe UHPLC ... EXP Reusable Fittings for HPLC & UHPLC ... Fittings: Reusable fittings for easy, reliable HPLC and UHPLC connections. ... for reliable UHPLC use up to 20,000+ psi! Patented ferrule can be installed repeatedly without...

EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing

EXP Hand-Tight Fittings

...+ bar)—no tools needed! For a reliable 20,000+ psi (1,400+ bar) UHPLC connection with either fitting instructions may result in unsafe UHPLC connections and/or non-ZDV connections.Hybrid Ferrule U ... EXP Reusable Fittings for HPLC & UHPLC ... Fittings: Reusable fittings for easy, reliable HPLC and UHPLC connections. ... ,700+ psi seal. Both hand-tight and hex-head styles wrench tighten for reliable UHPLC use up to 20...

Ultra Bulk Packing Materials (5 µm)

Use our bulk packing materials to pack your own columns!

...Chromatography Columns , HPLC & UHPLC Columns , Bulk Packing Materials...

Restek Pack in a Box Kit

...Chromatography Columns , HPLC & UHPLC Columns , Bulk Packing Materials...

High-Pressure Frit-Type In-Line Filter

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Roc LC Guard Column Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Trident HPLC In-Line Guard Cartridge Holders

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Viva Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Ultra Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Allure Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Pinnacle DB Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Ultra Aromax Columns (USP L11)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc C8 HPLC Columns (USP L7)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc C18 HPLC Columns (USP L1)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc Phenyl-Hexyl HPLC Columns (USP L11)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc Cyano HPLC Columns (USP L10)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Roc Silica HPLC Columns (USP L3)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva C18 Columns (USP L1)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva C8 Columns (USP L7)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva C4 Columns (USP L26)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva PFP Propyl Columns (USP L43)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Viva Biphenyl Columns (USP L11)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Biphenyl Columns (USP L11)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra PFP Propyl Columns (USP L43)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra IBD Columns (USP L68)

Specialized Columns for Mixed Polar and Nonpolar Compounds

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C8 Columns (USP L7)

Excellent for a wide range of analyses

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra C18 Columns (USP L1)

Excellent for a wide range of analyses

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Cyano Columns (USP L10)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Aqueous C18 Columns (USP L1)

...Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor EtG/EtS LC Column

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns at www ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor Biphenyl LC Columns (USP L11)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns at www ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor FluoroPhenyl LC Columns (USP L43)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns at www ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Force EXP Guard Column Cartridges

... pressure: 600 bar/8,700 psi.Apply Force LC columns to all of your HPLC and UHPLC instrument ... Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... UltraShield filter.Apply Force LC columns to all of your HPLC and UHPLC instrument...

Raptor C18 LC Columns (USP L1)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns at www and UHPLC instrumentation to run faster. Get there with the only general-purpose C18 that gives you ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Raptor EXP Guard Column Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware ... .For 1.8 µm Raptor columns, use an UltraShield UHPLC PreColumn Filter.Raptor SPP LC columns...

Ultra Silica HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra C18 HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra C8 HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra Cyano HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra IBD HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra Biphenyl Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

Ultra PFP Propyl Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

EXP Direct Connect Holder

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Replacement Cap Frit Filters for Trident Guard Cartridges

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Raptor ARC-18 LC Columns (USP L1)

... analysis times with existing HPLC and UHPLC instrumentation. Learn more about Raptor LC columns at www ... Chromatography Columns , HPLC & UHPLC Columns , HPLC & UHPLC Analytical Columns...

Ultra Aqueous C18 HPLC Prep Columns

...Chromatography Columns , HPLC & UHPLC Columns , Prep Columns...

PAH Interferences Standard (5 components)

... with Pinnacle II PAH columns or UHPLC compatible on the Pinnacle DB PAH column. ... with Pinnacle II PAH columns or UHPLC compatible on the Pinnacle DB PAH column. ...

Thomson SINGLE StEP Standard Filter Vials

... autosamplers, including UHPLC instruments. Patent No. 7,790,117 ... autosamplers, including UHPLC instruments. ...

Trident LC Column Protection System

Redesigned to be more rugged and easier to use!

...Chromatography Columns , HPLC & UHPLC Columns , Guard Cartridges & Hardware...

Flexible Premium Grade Stainless Steel Tubing

... Ideal for all plumbing in UHPLC systems. 1...

Stainless Steel Tubing

... Ideal for all plumbing in UHPLC systems. 1...

Thomson SINGLE StEP Low Evaporation Filter Vials

... dead volume (120 μL). Fit most standard 12 x 32 mm autosamplers, including UHPLC instruments. ...

EU 15+1 PAH Standard (16 components)

... compatible when diluted in appropriate solvent and analyzed with Pinnacle II PAH columns or UHPLC compatible ... with Pinnacle II PAH columns or UHPLC compatible on the Pinnacle DB...

Thomson SINGLE StEP eXtreme Filter Vials

... autosamplers, including UHPLC instruments. ...

Thomson SINGLE StEP Nano Filter Vials

... autosamplers, including UHPLC instruments. ...

Opti-Max Check Valves

... with the Opti-Max check valve system.*UHPLC cartridges rated to 20,000 psi (1,400 bar)Hybrid...

UHPLC Filters: Protect Your Column and Chromatographic Performance (PDF 476kB)

...UHPLC Filters Protect Your Column and Chromatographic Performance UltraShield UHPLC Precolumn Filter • Economical protection against microparticles. • Extend UHPLC column lifetime. • Negligible ... precolumn filter, UltraShield, UltraLine, UHPLC...

The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses (PDF 878kB)

... Particles (SPP) for UHPLC Analyses By Scott Grossman, Susan Steinike, Dan Li, and Justin Steimling of Raptor 1.8 μm columns, this superior Raptor performance has been unleashed on your UHPLC analyses. But ... 1.8, Raptor, UHPLC...

USLC Columns—Ultra Selective Liquid Chromatography

Selectivity is the most influential factor affecting peak separation, or resolution. Restek Ultra Selective Liquid Chromatography (USLC) technology i...

Force LC Columns Brochure (PDF 4515kB)

...-lasting and reproducible—even with rapid cycling and elevated UHPLC pressures. • Fully scalable between HPLC and UHPLC -edge UHPLC you just plumbed last week. This incredibly rugged and supremely consistent column line ... Force LC columns, HPLC, UHPLC...

Force LC Columns Sales Sheet (PDF 3547kB)

...—even with rapid cycling and elevated UHPLC pressures. • Fully scalable between HPLC and UHPLC—easily transfer -edge UHPLC you just plumbed last week. This incredibly rugged and supremely consistent column line ... Force LC columns, HPLC, UHPLC...

UltraShield UHPLC Precolumn Filter Instruction Sheet (PDF 219kB)

...UltraShield UHPLC Precolumn Filter cat.# 24995, 24996, 24997, 25809, 25810, 25811 Ultra Shield precolumn filters are designed to be an affordable UHPLC precolumn filter with a total volume of less than...

EXP and EXP2 Fittings: Reusable fittings for easy, reliable HPLC & UHPLC connections (PDF 694kB)

..., reliable HPLC & UHPLC connections www.restek.com • Patented ferrule can be installed repeatedly without , reliable HPLC & UHPLC connections for 10-32 fittings and 1/16" tubing PEEK End: Proven sealing ability...

EXP Fittings Instruction Sheet (PDF 707kB)

...EXP fittings Reusable fittings for easy, yet reliable HPLC & UHPLC connections for 10 to 8,700+ psi (600+ bar)—no tools needed! For a reliable 20,000+ psi (1,400+ bar) UHPLC connection...

Increase Data Quality for Sulfonamide Antibiotics Analysis by HPLC and UHPLC (PDF 975kB)

...Food Safety Applications Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC between UV and MS detection. • Maximize sample throughput by combining USLC selectivity with UHPLC speed...

The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses

... on your UHPLC analyses. But, what exactly does that mean? In this technical note, we will explore the benefits of using Raptor 1.8 µm LC columns in your UHPLC instrument with a focus on peak capacity, column ... and for their ability to help existing HPLC customers achieve faster separations without the need to purchase UHPLC on your UHPLC analyses. But, what exactly does that mean? In this technical note, we will explore ... Raptor, 1.8, UHPLC...

Novel Column Chemistry—High Impact, Low Cost Technology

... industry are the implementation of fast LC (UHPLC), the increased applicability of LC mass spectrometry (LC in laboratory design. Without question, the higher sample throughput of UHPLC and the data quality of LC ... reversed phase, biphenyl, RPLC, UHPLC...

Dissecting Raptor LC Columns: A closer look at a new species (PDF 1725kB)

... with existing HPLC and UHPLC instrumentation. • Long-lasting ruggedness for dependable reproducibility with small-particle technology, SPP also provides an efficiency boost to those running UHPLC when compared...

Column Choice: A Critical Factor for Successful UHPLC Integration

...Ultra High Pressure Liquid Chromatography (UHPLC) is an increasingly popular platform face is seamlessly integrating methods developed using UHPLC into pharmaceutical labs, which are still ... Column choice is a critical factor in successfully transferring methods between UHPLC and HPLC . Here, we discuss the column qualities that contribute to the successful integration of UHPLC technology. ... scaling methods, accuracy, method transfer, HPLC, UHPLC...

USLC Columns: Choose Columns Fast. Develop Methods Faster. (PDF 1815kB)

... method development. Figure 3: Restek offers the widest range of phase selectivities for HPLC and UHPLC –21.2 mm Prep 30–50 mm Column Line* Particle Diameter Use Pinnacle DB 1.9 μm UHPLC Ultra 3 and 5 ... , method development, UHPLC, HPLC, USLC, reversed phase...

EXP2 Fittings Instruction Sheet (PDF 217kB)

.... • Suitable for both HPLC and UHPLC. WARNING: Do not use EXP ferrules with standard nuts. Failure to use EXP fittings according to these instructions may result in unsafe UHPLC connections and/or non...

Force LC Columns

Force LC columns are long-lasting and reproducible, even under the stress of UHPLC. Premium quality is backed by the strength of our 100% Pure Satisfaction guarantee. And perhaps most important, Force LC columns are fully scala...

USLC Columns Put the Right Tools in Your LC Method Development Toolbox (PDF 1474kB)

... Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column significant selectivity differences, you can drastically speed up HPLC and UHPLC method development ... , reversed phase, H-S Model, method development, UHPLC, HPLC...

Raptor SPP C18 Columns Sales Sheet (PDF 1888kB)

... columns to last longer, data to be reproducible, and existing HPLC and UHPLC instrumentation to run % B), 3.50 min (20% B); Flow: 1.0 mL/min; Detector: PDA @ 254 nm; Instrument: UHPLC. LC_PH0532...

Fast, Simple LC-MS/MS Analysis of Creatine and Creatinine (PDF 568kB)

...Shield UHPLC precolumn filter 0.2 μm (cat.# 25810); Temp.: 40 °C; Sample: Diluent: 20:80 Water ; Instrument: UHPLC; Notes: Sample Preparation: Endogenous levels of creatinine and creatine in human plasma...

High-Throughput LC-MS/MS Analysis of Phosphatidylethanol in Whole Blood (PDF 561kB)

...:1 Methanol:2-propanol Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC Notes Sample Preparation .6 mm cat.# Raptor FluoroPhenyl EXP Guard Column Cartridge UHPLC 3-pk. 9319U0252 9319U0253 Raptor...

ChromaBLOGraphy: Finding the right fitting for HPLC or UHPLC

If you are wondering which type of fitting you need, you can find a wealth of information in the Frequently Asked Questions (FAQs) section of our website, under the HPLC and UHPLC category. Click on the links below for answers to some common questions: What size threads are on the end fittings of my HPLC […]

Frequently Asked Questions: HPLC and UHPLC

... is best for my lab? Is there a Restek guard cartridge to go with my UHPLC polymer-based fittings? Which fittings can be used for UHPLC...

Raptor ARC-18 LC Columns Sales Sheet (PDF 1872kB)

... UltraShield UHPLC PreColumn Filter—for 1.8 μm Raptor Columns Description Filter Porosity qty. cat .# UltraShield UHPLC PreColumn Filter 0.2 μm frit ea. 25809 5-pk. 25810 10-pk. 25811 EXP Reusable...

Raptor FluoroPhenyl LC Columns Sales Sheet (PDF 1816kB)

...L/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC. Column: Raptor FluoroPhenyl (cat.# 9319A1E L/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC. Easily separate compounds that coelute...

4-Minute Bisphenol A (BPA) Analysis Increases Sample Throughput (PDF 402kB)

... separation regardless of instrument platform—compatible with both HPLC and UHPLC systems. Bisphenol A (BPA . In addition, Raptor Biphenyl columns can be used with both conventional HPLC and UHPLC instruments. For BPA...

Raptor FluoroPhenyl LC Columns Brochure (PDF 3042kB)

... instruments. Raptor then evolved to bring that same improved speed, efficiency, and selectivity to UHPLC L/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC. Peaks tR (min) Precursor Ion Product Ion 1...

Rapid Analysis of 17 Bile Acids in Human Plasma by LC-MS/MS (PDF 767kB)

... minimum resolution of isomers. In this study, a simple UHPLC method was developed for the simultaneous on a Shimadzu Nexera UHPLC paired with a Shimadzu LCMS- 8060 MS/MS. Instrument conditions were as follows...

Raptor Biphenyl LC Columns Sales Sheet (PDF 3731kB)

... Ferrule— ZDV Connections! Raptor EXP Guard Column Cartridges EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing EXP Direct Connect Holder UltraShield UHPLC PreColumn Filter—for 1...

Raptor Biphenyl LC Columns Brochure (PDF 6071kB)

... instruments. Raptor then evolved to bring that same improved speed, efficiency, and selectivity to UHPLC are rugged enough to last well past 1,000 injections, even in the high-pressure conditions of UHPLC. Column...

Direct Analysis of Morphine, M3G and M6G Metabolites, and Related Compounds in Urine by LC-MS/MS (PDF 778kB)

... compounds was conducted on a Shimadzu Nexera UHPLC equipped with a SCIEX API 4500 MS/MS. Detection equipped with an UltraShield UHPLC precolumn filter with a 0.2 μm frit. Instrument conditions are detailed...

All Matrix Interferences Resolved (PDF 1706kB)

...: 2.7 μm; Guard Column: UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809); Temp.: 35 °C ! Description Filter Porosity qty. cat.# UltraShield UHPLC PreColumn Filter 0.2 μm frit ea. 25809 5-pk...

Restek LC Columns and Accessories (PDF 16883kB)

... Overview: Premium, long-lasting, and reproducible columns for HPLC and UHPLC; fully scalable from 1.8 μm to 3 μm to 5 μm. Ideal for: Scaling methods between HPLC and UHPLC. Instruments supported: • Agilent...

Raptor C18 LC Columns Brochure (PDF 2375kB)

... that same improved speed, efficiency, and selectivity to UHPLC analyses by offering 1.8 μm particle columns % B), 3.50 min (20% B); Flow: 1.0 mL/min; Detector: PDA @ 254 nm; Instrument: UHPLC. LC_PH0532...

USLC Columns Put the Right Tools in Your LC Method Development Toolbox Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection

..., you can drastically speed up HPLC and UHPLC method development by instead focusing on column choice instrumentation—especially since the recent advent of UHPLC—and end up choosing columns rather hastily, either ... , or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method development ... , "method development", USLC, "reversed phase", UHPLC, HPLC, "H-S Model"...

Reliable HILIC LC-MS/MS Analysis of 4-Methylimidazole (4-MEI) on Raptor FluoroPhenyl Columns (PDF 1191kB)

... acid in acetonitrile Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC LC_FF0561 Peaks t acid in acetonitrile Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC © 2018 Restek...

Raptor ARC-18 LC Columns Brochure (PDF 4942kB)

... then evolved to bring that same improved speed, efficiency, and selectivity to UHPLC analyses by offering 1 to bioanalytical work, whether you use traditional HPLC or UHPLC instruments, we’re all looking to simplify setup...

Increase Data Quality for Sulfonamide Residue Analysis by HPLC and UHPLC Using Unique Biphenyl Column Selectivity

... sample throughput by combining USLC selectivity with UHPLC speed. The analysis by a Biphenyl HPLC or UHPLC column can significantly improve data quality and reporting accuracy ... of sulfonamide residues and benefit both UV and MS detection. In addition, Biphenyl columns in a UHPLC format...

Raptor HILIC-Si: Simplify the Switch to HILIC (PDF 2013kB)

... for increasing sensitivity and selectivity in LC-MS analyses. • Fully compatible with both HPLC and UHPLC : 600 bar/8,700 psi Properties: • Compatible with both HPLC and UHPLC instruments. • Restek’s 2.7 μm...

One Analysis, One Column, Less than 9 Minutes for Over 60 Multiclass Antibiotics (PDF 903kB)

... Detector MS/MS Ion Mode: ESI+/ESIMode: Scheduled MRM Instrument UHPLC Notes 1. Positive and negative B: 0.1% Formic acid in acetonitrile Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC...

Thomson SINGLE StEP Filter Vials (PDF 738kB)

... autosamplers, such as Agilent, Shimadzu, and Waters—even UHPLC instrumentation. • Easy-to-use vials offer fast UHPLC instruments. Figure 1: The patented design of Thomson SINGLE StEP filter vials allows you...

Diagnosing and Preventing High Back Pressure Problems in LC Systems (PDF 5850kB)

...’s a fact of life for HPLC and UHPLC analyses. The force that is necessary to push a liquid mobile phase dead volume. • Fit most standard 12 x 32 mm autosamplers, including UHPLC instruments. www...

EXP Direct Connect Holder Instruction Sheet (PDF 897kB)

... tightening, but the resulting UHPLC connection will still perform well. For best results, tighten a maximum...

High-Throughput Analysis of Mycotoxins in Cannabis CBD Oil Pairs Simplified Cleanup with LC-MS/MS Sensitivity (PDF 1230kB)

....00 min (65%); Flow: 0.7 mL/min; Detector: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: 0 .# 5 x 4.6 mm cat.# Raptor Biphenyl EXP Guard Column Cartridge UHPLC 3-pk. 9309U0252 9309U0253...

Restek Essentials: Featured Chromatography Products 2018.1 (PDF 5439kB)

... autosamplers, including UHPLC instruments. Samples available! Contact your local Restek representative Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing WARNING: Do not use EXP ferrules...

A Fast Dilute-And-Shoot Method for Simultaneous 5-Hydroxyindoleacetic Acid (5-HIAA), Vanillylmandelic Acid (VMA), and Homovanillic Acid (HVA) LC-MS/MS Analysis in Human Urine (PDF 631kB)

...: Methanol Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC Notes The synthetic urine (Surine : ESIMode: MRM Instrument UHPLC Notes The human urine sample is the Bio-Rad Lyphochek quantitative urine...

Fast, Accurate LC-MS/MS Method for Folate Deficiency Biomarkers in Plasma (PDF 513kB)

... Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: Sample Preparation Method: 1. Aliquot 380 μ /Ferrule) 10-pk. 25938 EXP Hand-Tight Nut (w/o Ferrule) ea. 25939 EXP Reusable Fittings for HPLC & UHPLC...

Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV (PDF 686kB)

... by HPLC provides fast results without the expense of UHPLC equipment. • Baseline resolution improves and accurately without investing in UHPLC equipment. LC_FF0529 Peaks 1. Benzo[c]fluorene 2. Cyclopenta...

News: New UHPLC Guard Cartridges Deliver Ultimate Protection for Restek’s 1.8 μm Raptor Columns

Raptor 1.8 μm LC columns unleashed superior Raptor performance on UHPLC analyses, and now Restek has released EXP UHPLC guard column cartridges made specifically for Raptor 1.8 μm columns. These new guards provide extreme protection from particulates and matrix contamination, … Continue reading

News: Restek Unleashes Superior Raptor Performance on Your UHPLC Analyses — Now with 1.8 μm Particles

Raptor LC columns have been providing higher efficiencies, faster analyses, and rock-solid reliability to HPLC users for several years. And they have just evolved again. Restek has brought the benefits of Raptor columns to those who have made the leap … Continue reading

News: Understanding Selectivity in Reversed Phase Separations – A Simplified Approach to HPLC and UHPLC Column Selection

Author(s): Rick Lake, Ty Kahler Published By: Restek Corporation Year of Publication: 2011 Link: http://www.restek.com/Technical-Resources/Technical-Library/Pharmaceutical/general_PHAN1348 Abstract: Column selectivity has the most significant influence on chromatographic peak separation, or resolution, so choosing the right column can greatly speed up HPLC and UHPLC method … Continue reading

Raptor SPP Core-Shell LC Columns

Raptor SPP LC columns offer higher efficiency for drastically faster analysis times, better selectivity for substantially improved resolution, increa...

Definitive EtG/EtS LC-MS/MS Analysis: A Rugged 4-min Method for High-Throughput Labs (PDF 631kB)

... column: Raptor EtG/EtS, 2.7 μm, 100 mm x 2.1 mm (cat.# 9325A12) Guard column: UltraShield UHPLC ) Dimensions: 100 mm x 2.1 mm ID Particle Size: 2.7 μm Pore Size: 90 Å Guard Column: UltraShield UHPLC...

Highly Selective LC-MS/MS Analysis of Mycotoxins from Multiple Classes (PDF 556kB)

...; Detector: MS/MS ; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC Length 2.1 mm cat.# 3.0 mm cat.# 4 & UHPLC for 10-32 fittings and 1/16" tubing EXP Hand-Tight Fittings Unidirectional Raptor EXP Guard...

Featured Application: Polycyclic Aromatic Hydrocarbons (PAHs) on a Pinnacle II PAH HPLC Column Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV

... ~15-minute analysis of PAHs by HPLC provides fast results without the expense of UHPLC equipment with a Pinnacle II PAH column allows labs to report PAH results quickly and accurately without investing in UHPLC ... minute analysis without the expense of UHPLC equipment....

News: The Effects of LC Particle Choice on Column Performance: Raptor 1.8 µm Superficially Porous Particles (SPP) for UHPLC Analyses

Published By: Restek Corporation Year of Publication: 2017 Link: www.restek.com/Technical-Resources/Technical-Library/General-Interest/gen_GNAR2803-UNV Abstract: Superficially porous particles (commonly referred to as SPP or “core-shell” particles) have been proven to provide fast and efficient LC separations. These particles feature a solid, impermeable core enveloped by a … Continue reading

Influencing Selectivity in LC-MS Peptides Analysis on the Raptor ARC-18 Column (PDF 989kB)

... Instrument UHPLC 3 www.restek.com Figure 3: Effects of Acid Concentration on Retention and Selectivity. LC Detector MS Ion Mode: ESI+ Mode: MS scan Instrument UHPLC 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4...

Overcoming the Effects of Highly Organic Protein Precipitation Extracts on LC Peak Shape Using Direct Injection (PDF 585kB)

... and undiluted extracts) were analyzed using a Shimadzu Nexera UHPLC equipped with a SCIEX API 4500 MS/MS under bar Detector MS/MS Ion Mode: ESI+ Mode: Scheduled MRM Instrument UHPLC Notes Sample Preparation...

More of What You Need to Keep Your LC Flowing: Fittings, tubing, mobile phase management & safety, and more. (PDF 3764kB)

.... 27713 PEEK-HT Hex-Head Fittings for HPLC and UHPLC • One-piece design; no ferrule required. • Use ,000 psi JR-T-6003-M10 ea. 27756 4 www.restek.com Stainless Steel Unions, Tees, and Crosses for UHPLC...

Tech Tip: Column Conditioning Ensures Consistent EtG/EtS Results (PDF 402kB)

... Size: 2.7 μm Pore Size: 90 Å Guard Column: UltraShield UHPLC precolumn filter, 0.2 μm frit (cat...

7.5-Min Screening Analysis of Multiresidue Pesticides in Brown Rice Flour (PDF 1543kB)

... exhibit recoveries between 70-120%. • Compatible with both traditional HPLC and modern UHPLC instruments M ammonium formate in methanol Detector MS/MS Ion Mode: ESI+/ESIMode: Scheduled MRM Instrument UHPLC...

Restek Advantage, 2012.1 (PDF 14846kB)

... residue testing…pp. 12–13 www.restek.com Also in this issue Sulfonamide residues via HPLC & UHPLC for Sulfonamides by HPLC and UHPLC Using Unique Biphenyl Column Selectivity...

Resprep PPT 96-Well Plates Sales Sheet (PDF 1620kB)

....1% formic acid Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Extraction Procedure: 1...

Method Development and Column Selection: How the FluoroPhenyl Phase Provides the Power of HILIC and Reversed-Phase Modes in One Column (PDF 2146kB)

...L/min) 0.00 08 20 0.8 Max Pressure: 400 bar Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC .00 0.8 95 5 Max Pressure: 400 bar Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC...

Analysis of Synthetic Cannabinoids and Metabolites: Adding New Compounds to an Existing LC-MS/MS Method (PDF 870kB)

... performed on a Waters ACQUITY UPLC I-Class equipped with a Xevo TQ-S and a Shimadzu Nexera UHPLC equipped + Mode: MRM Instrument UHPLC Precursor Quantifer Qualifier Peaks tR (min) Ion Product Ion Product Ion...

LC-MS/MS Analysis of Urinary Free Cortisol and Cortisone without Matrix Interferences (PDF 515kB)

... Pressure: 400 bar Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes: Sample Preparation...

Simplified Methylmalonic Acid Analysis without Derivatization in Human Plasma by LC-MS/MS (PDF 584kB)

...,700 psi. Apply Force LC columns to all of your HPLC and UHPLC instrument platforms at www...

Simplified Methylmalonic Acid Analysis without Derivatization in Human Plasma by LC-MS/MS (PDF 584kB)

...,700 psi. Apply Force LC columns to all of your HPLC and UHPLC instrument platforms at www...

Trace-Level Analysis of Melamine and Related Compounds by LC-MS/MS (PDF 763kB)

.... EXP Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing EXP Hand-Tight Fittings...

Fast, Sensitive LC-MS/MS Analysis of GHB and Related Compounds in Human Blood for Forensic Testing (PDF 630kB)

... LC columns to all of your HPLC and UHPLC instrument platforms at www.restek.com/force Hybrid Ferrule...

Rapid and Accurate LC-MS/MS Analysis of Nicotine and Related Compounds in Urine Using Raptor Biphenyl LC Columns and MS-Friendly Mobile Phases (PDF 1244kB)

.../MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes The urine from a non-tobacco user was fortified Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Samples were processed with a liquid...

Rapid Analysis of C3 Epimers of 25-Hydroxyvitamin D in Serum by LC-MS/MS (PDF 559kB)

....01 0.6 25 75 7.00 0.6 25 75 Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Sample UHPLC Notes Serum (400 μL) was mixed with 0.2 M ZnSO₄ (400 μL) in a 4 mL glass vial. Then, 800 μ...

Restek Advantage, 2011.1 (PDF 3826kB)

... columns are offered on 1.9µm silica.Labs analyzing PAHs byeither HPLC or UHPLC will benefit from Restek USLC columns, available in both HPLC and UHPLC formats, offer the widest rangeof selectivities...

Comprehensive Pesticide Residue Analysis by LC/MS/MS Using an Ultra Aqueous C18 Column (PDF 1039kB)

... methods. Ultra-high pressure LC (UHPLC) can also be used with MS/MS detection for monitoring pesticide residues. UHPLC allows for higher sample throughput when used in conjunction with a highly efficient ...

“The Big Pain”: Development of Pain-Free Methods for Analyzing 231 Multiclass Drugs and Metabolites by LC-MS/MS (PDF 4733kB)

...: Scheduled MRM Instrument UHPLC Notes 1. Positive and negative polarity data were collected...

Comprehensive LC-MS/MS Analysis of 15 Bisphenols in 8 Minutes (PDF 519kB)

...: Methanol Detector MS/MS Ion Mode: ESIMode: MRM Instrument UHPLC LC_FS0518 www.restek.com 3 Length 2...

Rapid Perfluorinated Alkyl Acid Analysis by LC-MS/MS Increases Sample Throughput (PDF 640kB)

.../MS Ion Mode: ESIMode: MRM Instrument UHPLC LC_EV0536 tR Conc. Precursor Product Peaks (min) (ng...

Improve Analysis of Aldehydes and Ketones in Air Samples with Faster, More Accurate Methodology (PDF 417kB)

...), Flow: 0.8 mL/min; Detector: UV/ Vis @ 365, 4.8 nm; Instrument: UHPLC; Notes: *Mobile phase B...

Trace-Level Analysis of Metanephrines in Plasma by HILIC LC-MS/MS (PDF 712kB)

....00 0.3 10 90 5.00 0.3 10 90 Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Solid 5.00 0.3 10 90 Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Solid Phase...

High-Throughput Analysis of Immunosuppressive Drugs from Whole Blood by LC-MS/MS (PDF 524kB)

... Max Pressure: 305 bar Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Sample + Mode: MRM Instrument UHPLC Notes Sample Preparation Procedure: Human whole blood (100 μL) was mixed...

USLC Column Selection & Mobile Phase Adjustment Guide (PDF 1682kB)

... Prep 30–50 mm Column Line* Particle Diameter Use Pinnacle DB 1.9 μm UHPLC Ultra 3 and 5 μm HPLC...

Featured Application: Fast Analysis of Bisphenol A on a Raptor Biphenyl LC Column 4-Minute Bisphenol A (BPA) Analysis Increases Sample Throughput

...—compatible with both HPLC and UHPLC systems. Bisphenol A (BPA) is a high . In addition, Raptor Biphenyl columns can be used with both conventional HPLC and UHPLC instruments. For BPA...

ChromaBLOGraphy: Analyze PAHs by HPLC without Acetonitrile

Labs that are trying to cope with the acetonitrile shortage are trying to reduce solvent usage, or going to an alternate mobile phase. One way to reduce solvent usage is to minimize your column volume. This means going to a narrower column and possibly a shorter column as well. Ultra high pressure liquid chromatography (UHPLC) [...]

ChromaBLOGraphy: Live Long and Prosper-How to make your HPLC columns last longer

We frequently get questions about how long an HPLC or UHPLC column should last. To borrow a phrase from my colleagues, “Your mileage may vary.”  Columns are like cars, so their lifetime depends how they are used and how well they are maintained. That being said, some phases are known to be more rugged than [...]

News: Apply Force to Your LC Methods with Restek’s New High-Performance Columns

Restek’s new Force performance LC columns give you the power to maximize instrument uptime, increase productivity, and transfer methods across your entire lab,  from your trusted-but-aged HPLC to the cutting-edge UHPLC you just plumbed last week. This incredibly rugged and … Continue reading

ChromaBLOGraphy: Choosing a guard cartridge holder for LC

If you need help choosing one of the Trident guard cartridge holders, check out our information found in the HPLC and UHPLC FAQs section of our website. The FAQs contain a lot of good information and are often overlooked. For most of our columns we prefer the Trident Direct Guard holder system, because of its […]

ChromaBLOGraphy: Mass Overload on Superficially Porous Particles

The benefits of superficially porous particles are without question.  Who wouldn’t want to perform faster separations without the need for expensive UHPLC instrumentation?  It sounds too good to be true.  There must be some drawback – right? The solid, impermeable core present in Raptor™ particles increases column efficiency by decreasing the diffusion path at the […]

ChromaBLOGraphy: What can I use to filter my mobile phase?

If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed […]

Analysis of Fentanyl and Its Analogues in Human Urine by LC-MS/MS (PDF 537kB)

....40 238.19 111.06 Detector MS/MS Ion Mode: ESI+ Mode: MRM Instrument UHPLC Notes Human urine...

Simultaneous Analysis of Catecholamines and Metanephrines in Urine by LC-MS/MS (PDF 1289kB)

...: ESI+ Mode: MRM Instrument UHPLC Notes Solid Phase Extraction Procedure: A 200 μL aliquot of urine...

Growing Analytical Solutions for Cannabis Testing: Innovative Products and Expertise for Fast, Accurate, Reliable Results (PDF 8739kB)

... your instrument performance so you won’t need to buy expensive UHPLC equipment or extend your capital analysis times for cannabinoids without the expense of UHPLC equipment. Applications PRODUCT POTENCY...

News: Keep Your LC Running Smoothly with New Accessories from Restek

Simplify maintenance with the latest LC accessories from Restek. Reusable EXP2 HPLC/UHPLC fittings allow repeated 20,000+ psi seals to be easily made without wrenches. The patented titanium/PEEK ferrule provides zero-dead-volume connections without compromising high-pressure seals. Their compact design makes them … Continue reading

News: Hydrogen as Carrier Gas: Always Available, Cost Effective and Fast – A Personal Reflection

Author: Jaap de Zeeuw Restek Corporation Published By: Chromatography Today Issue: November / December 2012 Year of Publication: 2012 Link: http://www.chromatographytoday.com/articles/hplc-uhplc-lc-ms/31/jaap_de_zeeuw/hydrogen_as_carrier_gas_always_available_cost_effective_and_fast_a_personal_reflection/1333/ Abstract: Hydrogen is on the agenda again. Since the supply of helium has become a challenge, many labs are … Continue reading

ChromaBLOGraphy: Which LC column should I use for my method?

We understand that with so many products on the market, choosing a column to get started can be difficult. Of course, column selection depends on what type of method you are following and what kind of LC system you have in your lab. Let’s begin by looking at our choices for the more traditional, fully […]

ChromaBLOGraphy: EXP Fittings: Which Nut Do I Need?

Our brilliant friends at Optimize Technologies are continually innovating and making our lab lives easier. We love their patented EXP Titanium Hybrid Ferrule technology that is truly universal. You can use it on any tubing, any 10-32-port, any instrument—wrench-tight to 20,000 psi. This fully UHPLC-capable EXP ferrule is used with all EXP nuts. Restek offers […]

ChromaBLOGraphy: Which LC Phase Do I Need?

So you’re thinking about doing HPLC (or UHPLC) and don’t have a method? You’ve never even seen a method for anything remotely similar. You might have even searched our HPLC chromatogram database for the application and haven’t found anything.  (After all, that’s the quickest and easiest way to get some ideas.) What to do… Let’s [...]

Big Pain Assays Aren't a Big Pain with the Raptor Biphenyl LC Column (PDF 2205kB)

...: ESI+/ESI-; Mode: Scheduled MRM; Instrument: UHPLC; Notes: 1. Positive and negative polarity data were...

ChromaBLOGraphy: What is the pressure limit for my LC column?

This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ROC LC columns, the maximum operating pressure is 400 […]

ChromaBLOGraphy: Choosing a guard cartridge for LC

So you have a guard cartridge holder of some sort, and you don’t have the guard cartridge to put inside. If you happen to have missed that last blog post about the holder, click on the following link. Choosing a guard cartridge holder for LC Let’s determine what type of guard cartridge you need. A […]

ChromaBLOGraphy: What is SPP and when should I use a Raptor column?

While this sounds like a very broad question, I’m sure it is crossing some of your minds. To start out, SPP stands for superficially porous particles. It has also been called “core-shell”.   If you are a veteran in the world of HPLC, this term is old hat for you. If you are new to the […]

ChromaBLOGraphy: How to decrease run time for reversed phase LC

There are several ways to accomplish this, although it boils down to roughly 4 possibilities. Use a shorter column length. This may be appropriate if you have plenty of retention for your analyte(s), if you only have one or a few analytes AND if you do not have interference peaks in your chromatogram. It does, […]

A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS (PDF 1398kB)

...: MS/MS; Ion Mode: ESI+; Mode: MRM; Instrument: UHPLC; Notes: Sample Preparation Procedure: A 500 μ...

Analytical Method for Polycyclic Aromatic Hydrocarbons (PAHs) in Yerba Mate Tea Using Modified QuEChERS, Solid Phase Extraction and GC-TOFMS and GC-MS/MS (PDF 634kB)

..., Determination of amantadine and rimantadine in chicken muscle by QuEChERS pretreatment method and UHPLC coupled...

News: Simplify the Switch to HILIC with Raptor HILIC-Si Columns

Restek is bringing the speed and reliability of Raptor LC columns into the HILIC realm with the addition of the Raptor HILIC-Si column. HILIC is an increasingly popular alternative to reversed-phase LC for challenging polar analytes because it provides better … Continue reading

News: New Raptor™ FluoroPhenyl Phase: the Power of HILIC and RP Modes in One LC Column

Restek has now extended the speed and reliability of Raptor™ column technology into the HILIC realm with the addition of Raptor™ FluoroPhenyl columns. Restek’s Raptor™ FluoroPhenyl phase offers chromatographers the ability to run in reversed-phase or HILIC mode for a … Continue reading

News: The New USLC® Toolbox Gives You All the Right Tools—All in One Box

Restek’s USLC® column set represents the widest range of reversed phase selectivity available with just 4 stationary phases: Aqueous C18, IBD, Biphenyl, and PFP Propyl. It simplifies column choice for fast, effective method development—and new USLC® toolboxes make things even … Continue reading

News: Liquid Chromatography’s Complementary Role to Gas Chromatography in Cannabis Testing

Authors: Justin Steimling, Ty Kahler Restek Corporation Published By: Supplement to LCGC North America Year of Publication:  June 2018 Volume, Number: Volume 36, Number s6 Link: http://files.pharmtech.com/alfresco_images/pharma/2018/06/13/f119cb54-ce43-4628-95f8-af1aa07bb6ff/LCGC_NAmerica_June2018Supp.pdf Abstract: The absence of consensus methods for cannabis testing is a challenging, but refreshing opportunity for … Continue reading

The Restek Biphenyl: Leading Resolution in LC since 2005

With ten years of unrivaled performance, Restek's time-tested Biphenyl has been proven to be the ideal LC column for clinical diagnostic, pain, pharm...

Direct Analysis of Morphine, M3G and M6G Metabolites, and Related Compounds in Urine by LC-MS/MS

... UHPLC equipped with a SCIEX API 4500 MS/MS. Detection was performed using electrospray ionization with a Restek Force Biphenyl 1.8 μm, 50 mm x 2.1 mm column equipped with an UltraShield UHPLC precolumn filter...

Restek Liquid Chromatography (LC) Columns — Cleaning Recommendations

... to the pump eluent. For UHPLC columns (1.9 µm), DO NOT FLIP THE COLUMN. Instead, flush...

ChromaBLOGraphy: Eastern Canada Trace Organic Workshop

Last month, I attended two trace environmental workshops at the National Water Research Institute (CCIW) in Burlington, Ontario; the Workshop on LC-MS/MS Applications in Environmental Analysis and Food Safety and the Eastern Canada Trace Organic Workshop (ECTOW). The full program can be found here. This was my first exposure to work done on Thermo’s Orbitrap, which [...]

News: New Raptor™ ARC-18 Columns Are Born for LC-MS/MS

The birth of Restek’s new Raptor™ SPP LC column line began with the innovative Biphenyl phase, and it has now grown to include a new Restek® phase: the ARC-18. Designed and intended specifically for use on LC-MS/MS systems, the Raptor™ … Continue reading

News: Struggling with EtG/EtS matrix interferences?

A Raptor EtG/EtS column is your solution! Typical methods for EtG and EtS analysis have several limitations: poor resolution from matrix components, long run times that limit sample throughput, and short column lifetimes. Restek has developed a new Raptor EtG/EtS … Continue reading

News: Raptor™ Speed, Efficiency, and Ruggedness is Now Available in C18

Every LC lab has a cache of C18s, but every C18 is not created equal. Because the chemistry tends to be similar, the silica support that carries this ubiquitous octadecylsilane phase becomes vitally important. When you need a general-purpose LC … Continue reading

ChromaBLOGraphy: Do you love your pet? Then you will love our Raptor™ Biphenyl LC column.

Have you ever faced this? Your pet has a stomach problem and nothing helps except to visit the veterinarian? Most likely, your beloved dog or cat is infected with parasitic protozoans, causing a disease called coccidiosis. Help is in sight; the veterinarian will use a pharmaceutical out of the group of coccidiostats and there is […]

ChromaBLOGraphy: Top 16 for 2016 – Restek LC Article Makes the Cut

If you are an avid chromatographer you likely subscribe to LC/GC magazine or its popular electronic version, E-Separation Solutions. It is definitely the place to go for news and information related to all things Chromatography. Over the years Restek has had many an article published in LC/GC and, full disclosure, we advertise there as well. Who […]

Rapid Analysis of 17 Bile Acids in Human Plasmaby LC-MS/MS

... minimum resolution of isomers. In this study, a simple UHPLC method was developed for the simultaneous LC-MS/MS analysis of bile acids in human plasma was performed on a Shimadzu Nexera UHPLC paired...

News: Experience Selectivity Accelerated With New Raptor™ SPP LC Columns and Guards

Restek is excited to announce the evolution of superficially porous particles with the introduction of Raptor™ LC columns. Superficially porous particles (commonly referred to as SPP or “core-shell” particles) changed the world of LC by dramatically boosting column efficiency and … Continue reading

ChromaBLOGraphy: How do I Replace my LC Tubing?

For those that are experienced with LC, you probably have this all figured out.  If that is the case, this might serve as a good introduction to Restek products that you can use for this.  For those that are newbies to liquid chromatography, welcome and I hope you find this useful! The best way to […]

ChromaBLOGraphy: Dilute, Shoot, and Elute – am I missing anything? YES!

  Everyone’s lab is different in terms of how many samples per day are processed, but they all share the common pain point of sample preparation. Some samples like blood and plasma need a significant amount of prep to remove proteins, phospholipids, and salts, whereas labs running urine or drinking water samples can “get by” […]

ChromaBLOGraphy: Resurrecting an old reverse phase LC column- should I, could I, how would I???

Well, if we had an Easter egg hunt and you found an egg that was from last year or the year before, you could probably tell the difference and you would know pretty quickly if it had gone bad.  Let’s just say that is not so easy with HPLC columns. If you’ve dealt with this […]

ChromaBLOGraphy: LC Columns: High-Pressure Survival of the Fittest!

As an R&D Chemist charged with developing the perfect packing method for new LC columns, one of the challenges is finding a packing method that results in a column which not only passes QC specifications but also passes our arduous lifetime analysis. During lifetime testing our columns are subjected to 1,000 continuous injections at 12,000 […]

Definitive EtG/EtS LC-MS/MS Analysis: A Rugged 4-Min Method for High-Throughput Labs

...: UltraShield UHPLC precolumn filter, 0.2 µm frit (cat.# 25809...

Diagnosing and Preventing High Back Pressure in LC Systems

... pressure. It’s a fact of life for HPLC and UHPLC analyses. The force that is necessary to push...

The Effects of LC Particle Choice on Column Performance: Switching from 3 and 5 µm Fully Porous Particles (FPP) to 5 µm Superficially Porous Particles (SPP)

... Performance Liquid Chromatography (UHPLC) instruments, thereby increasing sample throughput without capital...

Analysis of Synthetic Cannabinoids and Metabolites: Adding New Compounds to an Existing LC-MS/MS Method

... I-Class equipped with a Xevo TQ-S and a Shimadzu Nexera UHPLC equipped with a SCIEX API 4500 MS...

AOAC 2018

Here’s a sneak peek at what we’ll be up to during this year’s show.

ChromaBLOGraphy: What is Different about Prep LC? (Versus Analytical HPLC)

  The goal of prep LC is not to produce the best looking chromatogram. Prep LC can be used to purify a material for manufacturing or research purposes, or it can also be used for sample cleanup prior to analytical measurement, such as in gel permeation chromatography. Most Restek customers are more involved with using […]

Overcoming the Effects of Highly Organic Protein Precipitation Extracts on LC Peak Shape Using Direct Injection

... All samples (10x diluted and undiluted extracts) were analyzed using a Shimadzu Nexera UHPLC equipped...

Comprehensive Pesticide Residue Analysis by LC-MS/MS Using an Ultra Aqueous C18 Column

... of choice for LC/MS/MS pesticide monitoring methods. Ultra-high pressure LC (UHPLC) can also be used with MS/MS detection for monitoring pesticide residues. UHPLC allows for higher sample throughput...

ChromaBLOGraphy: What does the certificate for my new LC column tell me? Why should I keep it?

You know those pieces of paper that you sometimes run across when you open a box containing a new column? Maybe you don’t have much time to look at it or it sometimes gets lost along the way. Let’s stop for a minute and look at what that information is and how it pertains to […]

SOFT

Learn more about what Restek will be doing at the Society of Forensic Toxicologists (SOFT) annual meeting.

ISC 2018

Here’s a sneak peek at what we’ll be up to during this year’s show.

ChromaBLOGraphy: Building up pressure on HPLC?

We’ve all experienced a pressure error on HPLC at some time or another. Here are my best suggestions on handling them. Establish ground rules-This will help to define the problem and distinguish if there really is one. 1.     Check your programmed max pressure limit. Be aware of what the operating limit is for your system and [...]

ChromaBLOGraphy: Should I use a 2.7 or 5 µm Raptor™ column?

“When you come to a fork in the road, take it.” Maybe that advice worked for Yogi Berra, but does not help much in decisions about your lab equipment. If you are not certain whether you should use a Raptor™ column, please click on the title below to read my previous blog post before proceeding. […]

ChromaBLOGraphy: More Technical Service “Red Flags” – LC

This post is the second of its kind pertaining to LC analysis, all of which are an extension of a series pertaining to “Red Flags” for GC analysis. These are situations and symptoms that tell us in the Tech Service group that something is just not right. As we discussed in the first post, Technical […]

ChromaBLOGraphy: The Clog Blog

Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen […]

Analytical Method for Polycyclic Aromatic Hydrocarbons (PAHs) in Yerba Mate Tea Using Modified QuEChERS, Solid Phase Extraction and GC-TOFMS and GC-MS/MS

... muscle by QuEChERS pretreatment method and UHPLC coupled with LTQ Orbitrap mass spectrometry, J...

For advanced chromatogram searching, please check out our Chromatogram Library Search Tool.

Cannabis Flower Sample on Raptor ARC-18 1.8 μm by UHPLC-UV

LC_GN0590

More Details

Parabens on Raptor C18

LC_PH0531

More Details